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Urinary aromatic acid excretion by fed and fasted sheep in relation to protein metabolism in the rumen

Published online by Cambridge University Press:  09 March 2007

A. K. Martin
A. K. Martin
Affiliation:
Hannah Research Institute, Ayr KA6 5HL
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Abstract

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1. Two adult wether sheep were maintained on a diet of hay and two on a diet of dried grass for 3 weeks before starvation for a period of 10 d. Urinary excretion of the following acids was determined when the animals were fed and when they were fasted: total diethyl ethersoluble acids of hydrolysed and unhydrolysed urine, hippuric acid, benzoic acid and phenylacetic acid. By the 5th day of fasting, urinary output of all acids had attained stable levels that did not change during the remaining starvation period. The output of all urine fractions except phenylacetic acid declined rapidly during the first 4 d of fasting: phenylacetic acid output by all sheep increased to a maximum during the first 4 d of fast and then declined to stable values (0·42–0·73 g/24 h) which were greater than those observed when the sheep were fed. It is concluded that prolonged retention of food and microbial residues in the digestive tract is responsible for the large output of phenylacetic acid in the urine of fasted sheep.

2. Solutions of casein which supplied between 6·3. and 26·5 g nitrogen/24 h were infused into the rumens (fifteen experiments) or abomasums (sixteen experiments) of eight adult wether sheep. Ruminal infusions of casein caused increments in the urinary excretion of diethyl ethersoluble acids and phenylacetic acid. Both these increments were described by linear regression equations (P < 0·001), the coefficients of which showed that 284 ± 44 and 220 ± 21 mg benzoic acid equivalent were excreted as diethyl ether-soluble acids and phenylacetic acid respectively per g casein N infused. The phenylacetic acid excreted was equivalent to 95% of the phenylalanine of the infused casein. No increments in urinary benzoic acid were observed. One sheep scoured when it was given an abomasal infusion of casein. This was the only animal to show any increment in urinary aromatic acids when casein was infused into the abomasum.

3. When four sheep were given two rations containing an excess of carbohydrate as sugarbeet pulp or rolled barley, 11 and 16% respectively of their phenylalanine intakes were excreted in the urine as phenylacetic acid. When the same sheep were given two rations containing an excess of N as linseed meal or field beans, 51 and 59% respectively of their phenylalanine intakes appeared in the urine as phenylacetic acid.

4. Methods for the determination of creatinine, and of benzoic, phenylacetic, 3-phenylpropionic, cinnamic, hippuric and phenaceturic acids are described.

5. It is suggested that the amount of phenylacetic acid excreted in the urine is a measure of the equilibrium occurring in the rumen between catabolism of phenylalanine and reutilization of the products of catabolism for phenylalanine synthesis.

Type
General Nutrition
Information
British Journal of Nutrition , Volume 30 , Issue 2 , September 1973 , pp. 251 - 267
Copyright
Copyright © The Nutrition Society 1973

References

Allison, M. J. (1965). Biochem. biophys. Res. Commun. 18, 30.CrossRefGoogle Scholar
Annison, E. F., Chalmers, M. I., Marshall, S. B. & Synge, R. L. M. (1954). J. agric. Sci., Camb. 44, 370.CrossRefGoogle Scholar
Blaxter, K. L. & Martin, A. K. (1962). Br. J. Nutr. 16, 397.CrossRefGoogle Scholar
Cheng, K. J., Krishnamurty, H. G., Jones, G. A. & Simpson, F. J. (1971). Can. J. Microbiol. 17, 129.CrossRefGoogle Scholar
Conway, E. J. (1947). Microdiffusion Analysis and Volumetric Error 2nd ed., p. 77. London: Crosby Lockwood & Sons Ltd.Google Scholar
Fishman, W. H. & Green, S. (1955). J. biol. Chem. 215, 527.CrossRefGoogle Scholar
Ford, J. H. (1949). J. Am. chem. Soc. 71, 3842.CrossRefGoogle Scholar
Harborne, J. B. (1967). Comprative Biochemistry of the Flavonoids p. 61, London and New York: Academic Press.Google Scholar
Harborne, J. R. & Simmonds, N. W. (1964). In Biochemistry of Phenolic Compounds p. 83 [Harbrone, J. B., editor]. London and New York: Academic Press.Google Scholar
Hungate, R. E. (1966). The Rumen and its Microbes p. 409. London and New York: Academic Press.Google Scholar
Mangan, J. L. (1972). Br. J. Nutr. 27, 261.CrossRefGoogle Scholar
Martin, A. K. (1969 a). Br. J. Nutr. 23, 389.CrossRefGoogle Scholar
Martin, A. K. (1969 b). Br. J. Nutr. 23, 715.CrossRefGoogle Scholar
Martin, A. K. (1970). Br. J. Nutr. 24, 943.CrossRefGoogle Scholar
Moldave, K. & Meister, A. (1967). Biochim. biophys. Acta 24, 654.CrossRefGoogle Scholar
Patton, S. & Kessler, E. M. (1967). J. Dairy Sci. 50, 1505.CrossRefGoogle Scholar
Scott, T. W., Ward, P. F. V. & Dawson, R. M. C. (1964). Biochem. J. 90, 12.CrossRefGoogle Scholar
Seakins, J. W. T. (1971). Clinica chim. Acta 35, 121.CrossRefGoogle Scholar
Sinha, S. H. & Gabrieli, E. R. (1968). Clinica chim. Acta 19, 313.CrossRefGoogle Scholar
Van der Heiden, C., Wadman, S. K., Ketting, D. & De Bree, P. K. (1971). Clinica chim. Acta 31, 133.CrossRefGoogle Scholar
Van der Heiden, C., Wauters, E. A. K., Ketting, D., Duran, M. & Wadman, S. K. (1971). Clinica chim. Acta 34, 289.CrossRefGoogle Scholar
Wainman, F. W. & Paterson, D. (1963). J. agric. Sci., Camb. 61, 253.CrossRefGoogle Scholar
Williams, C. M. & Sweeley, C. C. (1964). In Biochemical Aspects of Gas Chromatography p. 238 [Szymanski, H. A., editor]. New York: Plenum Press.Google Scholar