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Effects of carvedilol and metoprolol on the myocardium during mechanical unloading in a rat heterotopic heart transplantation model

Part of: Basic Science

Published online by Cambridge University Press:  22 March 2021

Geena Kim Open the ORCID record for Geena Kim [Opens in a new window] ,
Hong Ryang Kil ,
Cheng Quan  and
Sang Su Lee
Geena Kim
Affiliation:
Department of Pediatrics, Chungnam National University Sejong Hospital, Chungnam National University School of Medicine, Sejong, Republic of Korea
Hong Ryang Kil
Affiliation:
Department of Pediatrics, Chungnam National University Hospital, Chungnam National University School of Medicine, Daejeon, Republic of Korea
Cheng Quan
Affiliation:
Department of Vascular Surgery, The Second Hospital of Jilin University, Changchun, Jilin, China
Sang Su Lee*
Affiliation:
Department of Surgery, Pusan National University Hospital, Yangsan, Republic of Korea
*
Author for correspondence: Sang Su Lee, MD, PhD, Department of Surgery, Vascular and Endovascular Division, Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, 20 Geumo Road, Yangsan, Gyeongsangnam50612, Republic of Korea. Tel: +82 55-360-2124; Fax: +82 55-360-2154. E-mail: phoenixdr@naver.com
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Abstract

Background and objectives:

Left ventricular assist devices enable recovery from severe heart failure and serve as a bridge to heart transplantation. However, chronic mechanical unloading can impair myocardial recovery. We aimed to assess myocyte size, fibrosis, apoptosis, and β-adrenoreceptor levels after rats with left ventricle unloading induced by heterotopic heart transplantation were administered carvedilol and metoprolol.

Methods:

Thirty rats with heart transplants were divided randomly into control, carvedilol treatment, and metoprolol treatment groups. Follow-up was conducted after 2 and 4 weeks of unloading.

Results:

Carvedilol and metoprolol treatments did not prevent the decrease in myocyte diameter in unloaded left ventricles. Metoprolol significantly decreased the ratio of the fibrotic area in the unloaded heart, measured using Masson’s trichrome staining after 2 weeks. However, carvedilol and metoprolol did not reduce apoptosis, based on measurements of terminal deoxynucleotidyl-transferase-mediated dUTP nick end-labelling positive cells and the expression of caspase-3 in unloaded hearts after 2 and 4 weeks. Metoprolol treatment did not significantly decrease the mRNA expression of myocardial SERCA2a in the unloaded heart after 2 weeks.

Conclusions:

Compared to carvedilol treatment, metoprolol treatment improved myocardial fibrosis and SERCA2a expression to a greater extent; however, neither drug prevented myocardial apoptosis.

Keywords

Carvedilolmetoprololtransplantationheterotopicmyocardium
Type
Original Article
Information
Cardiology in the Young , Volume 31 , Issue 8 , August 2021 , pp. 1269 - 1274
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Copyright
© The Author(s), 2021. Published by Cambridge University Press

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