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Evolution of some biochemical markers of growth in relation to osteoarticular status in young horses: results of a longitudinal study in three breeds

Published online by Cambridge University Press:  01 February 2007

J P Valette ,
C Robert ,
M P Toquet ,
J M Denoix  and
G Fortier
J P Valette*
Affiliation:
UMR INRA-ENVA, Biomécanique et Pathologie Locomotrice du Cheval, École Nationale Vétérinaire d'Alfort, 7, Avenue du Général-de-Gaulle, 94704 Maisons-Alfort Cedex, France
C Robert
Affiliation:
UMR INRA-ENVA, Biomécanique et Pathologie Locomotrice du Cheval, École Nationale Vétérinaire d'Alfort, 7, Avenue du Général-de-Gaulle, 94704 Maisons-Alfort Cedex, France
M P Toquet
Affiliation:
Laboratoire Départemental Frank Duncombe, 14053 Caen Cedex, France
J M Denoix
Affiliation:
UMR INRA-ENVA, Biomécanique et Pathologie Locomotrice du Cheval, École Nationale Vétérinaire d'Alfort, 7, Avenue du Général-de-Gaulle, 94704 Maisons-Alfort Cedex, France
G Fortier
Affiliation:
Laboratoire Départemental Frank Duncombe, 14053 Caen Cedex, France
*
*Corresponding author: jpvalette@vet-alfort.fr
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Abstract

Osteocalcin (OC), bone fraction of alkaline phosphatases (BAP) and hydroxyproline (HOP) are markers of bone cell activity. The kinetics of these markers and the analysis of their variations could be related to the osteoarticular status (OAS) of young horses. The growth of Thoroughbreds, French Trotters and Selle Français horses was followed up to 18 months. Blood samples were taken regularly to measure OC, HOP and BAP by standardized techniques. The OAS was evaluated by radiographic examination of the limbs. Based on radiographic findings, two groups of horses were investigated, with no lesions or severely affected. Analysis of variance was used to detect the effects of age and breed, and OAS on parameters. The logarithmic model was used to determine the kinetics of the markers. A rapid decrease in marker concentrations with age and differences between breed was observed. At birth, BAP, OC and HOP concentrations were significantly higher in normal horses (1910 UI l− 1, 192 ng ml− 1 and 35 mg l− 1, respectively) than in horses with severe lesions (1620 UI l− 1, 149 ng ml− 1 and 24 mg l− 1, respectively). During the first 6 months, OC, HOP and BAP remained lower in severely affected horses.

Keywords

horsedevelopmental orthopaedic diseaseradiographic examinationmarker of bone cell activitylongitudinal study
Type
Research Paper
Information
Equine and Comparative Exercise Physiology , Volume 4 , Issue 1 , February 2007 , pp. 23 - 29
Copyright
Copyright © Cambridge University Press 2007

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References

1McIlwraith, CW (2005). Use of synovial fluid and serum biomarkers in equine bone and joint disease: a review. Equine Veterinary Journal 37: 473–482.CrossRefGoogle ScholarPubMed
2Price, PA and Baukol, SA (1980). 125-dihydroxyvitamin D3 increases synthesis of the vitamin K-dependent bone protein by osteosarcoma cells. Journal of Biological Chemistry 255: 11660–11663.Google Scholar
3Davicco, MJ, Faulconnier, Y, Coxam, V, Dubroeucq, H, Martin-Rosset, W and Barlet, JP (1994). Systemic bone growth factors in light breed mares and their foals. Archives Internationales de Physiologie, de Biochimie et de Biophysique 102: 115–119.Google Scholar
4Mäenpää, PH, Pirskanen, A and Koskinen, E (1988). Biochemical indicators of bone formation in foal after transfer from pasture to stables for the winter months. American Journal of Veterinary Research 49: 1990–1992.Google Scholar
5Glade, M, Gupta, S and Reimers, TJ (1984). Hormonal responses to high and low planes of nutrition in weanling Thouroughbreds. Journal of Animal Science 5: 658–665.CrossRefGoogle Scholar
6McIlwraith, CW (1986). Proceedings of the AQHA Developmental Orthopaedic Disease Symposium. Amarillo, TX: AQHA, pp. 1–77.Google Scholar
7Jeffcott, LB and Henson, FMD (1998). Studies on growth cartilage in the horse and their application to aetiopathogenesis of dyschondroplasia (osteochondrosis). The Veterinary Journal 156: 177–192.Google Scholar
8Jeffcott, LB (2005). Developmental diseases affecting growing horse. The Growing Horse: Nutrition and Prevention of Growth Disorders 114 (pp. 243–255). Rome: EAAP publication.Google Scholar
9Delmas, PD (1993). Biochemical markers of bone turnover. Journal of Bone and Mineral Research 8: S549–S555.CrossRefGoogle ScholarPubMed
10Lepage, OM, Carstanjen, B and Uebelhart, D (2001). Non-invasive assessment of equine bone: an update. The Veterinary Journal 161: 10–23.Google Scholar
11Price, JS, Jackson, BF, Gray, JA, Harris, PA, Wright, IM, Pfeiffer, DU, Robins, SP, Eastell, R and Ricketts, SW (2001). Biochemical markers of bone metabolism in growing Thoroughbreds: a longitudinal study. Research in Veterinary Science 71: 37–44.CrossRefGoogle ScholarPubMed
12Carstanjen, B, Amory, H, Sulon, J, Hars, O, Remy, B, Langlois, P and Lepage, OM (2005). Serum osteocalcin and CTX-MMP concentration in young exercising Thoroughbred racehorses. Journal of Veterinary Medicine Series A 52: 114–120.CrossRefGoogle ScholarPubMed
13Black, A, Schoknecht, PA, Ralston, SL and Shapes, SA (1999). Diurnal variation and age differences in biochemical markers of bone turnover in horses. Journal of Animal Science 77: 75–83.Google Scholar
14Lepage, OM, Marcoux, M and Tremblay, A (1990). Serum osteocalcin or bone Gla-protein a biochemical marker for bone metabolism in horses: differences in serum levels with age. Canadian Journal of Veterinary Research 5: 223–226.Google Scholar
15Lepage, OM, Eicher, R, Uebelhart, B and Tschudi, P (1997). Influence of type and breed of horses on serum osteocalcin concentration and evaluation of the applicability of a bovine RIA and a human IRMA. American Journal of Veterinary Research 5: 48–57.Google Scholar
16Price, JS, Jackson, BF, Eastell, R, Goodship, AE, Blumsohn, A, Wright, I, Stoneham, S, Lanyon, LE and Russell, RGG (1995). Age related changes in biochemical markers of bone metabolism in horses. Equine Veterinary Journal 27: 201–207.Google Scholar
17Robert, C, Valette, JP and Denoix, JM (2006). Correlation between routine radiographic findings and early racing career in French Trotters. Equine Veterinary Journal Supplement 36: 473–478.Google Scholar
18Hoppe, F (1984). Radiological investigations of osteochondrosis dissecans in Standardbred Trotters and Swedish Warmblood horses. Equine Veterinary Journal 1: 425–429.Google Scholar
19Pool, RR (1993). Difficulties in definition of equine osteochondrosis; differentiation of developmental and acquired lesions. Equine Veterinary Journal Supplement 16: 5–12.CrossRefGoogle Scholar
20Jaeschke, G (1975). Routine determination of free hydroxyproline in horse serum methods and normal values. Zentralblatt für Veterinarmedecin A 22: 89–101.CrossRefGoogle ScholarPubMed
21Delmas, PD, Christiansen, C, Mann, KG and Price, PA (1990). Bone Gla Protein (Osteocalcin) Assay standardization report. Journal of Bone and Mineral Research 5: 5–11.CrossRefGoogle ScholarPubMed
22Hank, AM, Hoffmann, WE, Sanecki, RK, Schaeffer, DJ and Dorner, JL (1993). Quantitative determination of equine alkaline phosphatase isoenzymes in foal and adult serum. Journal of Veterinary Internal Medecine 7: 20–24.Google Scholar
23Billinghurst, RC, Brama, PA, van Weeren, PR, Knowlton, MS and McIlwraith, CW (2005). Evaluation of serum concentrations of biomarkers of skeletal metabolism and results of radiography as indicators of severity of osteochondrosis in foals. American Journal of Veterinary Research 65: 143–150.CrossRefGoogle Scholar
24Jackson, BF, Lonnell, C, Verheyen, KL, Dyson, P, Pfeiffer, DU and Price, JS (2005). Biochemical markers of bone metabolism and risk of dorsal metacarpal disease in 2-year-old Thoroughbreds. Equine veterinary Journal 37: 87–91.Google Scholar
25Ducy, P, Desbois, CH, Boyce, B, Pinero, G, Story, B, Dunstan, C, Smith, E, Bonadio, J, Goldstein, S, Gundberg, C, Bradley, A and Karsenty, G (1996). Increased bone formation in osteocalcin-deficient mice. Nature 38: 448–452.Google Scholar
26Jackson, BF, Blumsohn, A, Goodship, AE, Wilson, AM and Price, JS (2003). Circadian variation in biochemical markers of bone cell activity and insulin-like growth factor-I in two-year-old horses. Journal of Animal Science 11: 2804–2810.Google Scholar
27May, SA, Wyn-Jones, G and Peremans, KY (1986). Importance of oblique views in radiography of the equine limb. Equine Veterinary Journal 18(1): 7–13.CrossRefGoogle ScholarPubMed
28van Weeren, PR, Sloet van Oldruitenborgh-Oosterbaan, M and Barneveld, A (1999). The influence of birth weight, rate of weight gain and final achieved height and sex on the development of osteochondrotic lesions in a population of genetically predisposed Warmblood foals. Equine Veterinary Journal Supplement 31: 26–30.Google Scholar