Hostname: page-component-848d4c4894-75dct Total loading time: 0 Render date: 2024-06-10T12:45:17.090Z Has data issue: false hasContentIssue false
  • English
  • Français

Mesure de l’activité antiradicalaire du jus et des peaux d’oranges tunisiennes par le radical DPPH

Published online by Cambridge University Press:  03 June 2006

Jamilla Kalthoum Cherif ,
Ines M’Rabet ,
Mourad El Habiri ,
Rym Abidi  and
Anne-Marie Albrecht-Gary
Jamilla Kalthoum Cherif
Affiliation:
Institut préparatoire aux études d'ingénieurs de Tunis, 1008 Monfleury, Tunis, Tunisie
Ines M’Rabet
Affiliation:
Faculté des sciences de Bizerte, Université du 7 novembre à Carthage, 7021 Jarzouna–Bizerte, Tunisie École Chimie Polymère Matériaux, Laboratoire de physico-chimie bioinorganique, UMR 7509 CNRS, 25, rue Becquerel, 67200 Strasbourg, France
Mourad El Habiri
Affiliation:
Faculté des sciences de Bizerte, Université du 7 novembre à Carthage, 7021 Jarzouna–Bizerte, Tunisie
Rym Abidi
Affiliation:
École Chimie Polymère Matériaux, Laboratoire de physico-chimie bioinorganique, UMR 7509 CNRS, 25, rue Becquerel, 67200 Strasbourg, France
Anne-Marie Albrecht-Gary
Affiliation:
Faculté des sciences de Bizerte, Université du 7 novembre à Carthage, 7021 Jarzouna–Bizerte, Tunisie
Get access

Abstract

Introduction. L’orange (Citrus aurantium sinensis) est une source de composés bioactifs : vitamines C et E, flavonoïdes (flavanes et flavanones glycosylés et polyméthoxylés). Pour valoriser les déchets des peaux d’orange, nous avons mesuré l’activité antiradicalaire du jus et des peaux sèches et fraîches de cinq variétés d’orangers de Tunisie afin d’évaluer leur concentration en composés antioxydants utilisables dans l’industrie. Matériel et méthodes. Le pouvoir antiradicalaire du jus et des extraits des peaux a été évalué en suivant, en fonction du temps, la disparition du radical stable DPPH• par spectrophotométrie d’absorption UV-visible à 515 nm. L’activité antiradicalaire mesurée a été exprimée en concentration efficace (CE50). La détermination du volume V50 de vitamine C d’une solution d’acide ascorbique de concentration connue et d’un volume V50 des extraits de jus et des peaux sèches et fraîches d’orange a permis d’estimer la concentration molaire et massique de vitamine C équivalente dans les extraits. Résultats. Les jus d’orange contiennent environ 400 mg·L–1 d’équivalent de vitamine C. Dans le cas de réactions très rapides avec le radical DPPH•, la totalité de l’activité antiradicalaire peut être attribuée à la vitamine C. Dans le cas de réactions plus lentes, en plus de la vitamine C, ce sont les polyphénols (citroflavonoïdes) qui contribuent à l’activité antioxydante totale. Les extraits méthanoliques des peaux fraîches (flavédo) contiennent plus de vitamine C que les extraits de peaux sèches. Conclusion. Les peaux d’oranges constituent d’immenses sources d’antioxydants qui peuvent trouver leur application dans différents domaines : agroalimentaire, cosmétique, pharmaceutique et autres.

Keywords

TunisiaCitrus sinensisfruitsorange juicepeelwaste utilizationnatural antioxidantsascorbic acidTunisieCitrus sinensisfruitsjus d'orangepelureutilisation des déchetsantioxydant naturelacide ascorbique
Type
Research Article
Information
Fruits , Volume 61 , Issue 2 , March 2006 , pp. 99 - 107
Copyright
© CIRAD, EDP Sciences, 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Steinmetz, K.A., Potter, J.D., Vegetables, fruit and cancer prevention: a review, J. Am. Diet. Assoc. 53 (1996) 536–543.
Liu, S., Lee, I.M., Ajani, U., Intake of vegetables rich in carotenoids and risk of coronary heart disease in men: the physicians’ health study, Int. J. Epidemiol. 30 (2001) 130–135. CrossRef
Joshipura, K.J., Hu, F.B., Manson, J.E., The effect of fruit and vegetable intake on risk for coronary heart disease, Ann. Intern. Med. 134 (2001) 1106–1114. CrossRef
Gerber, M., Boutron-Ruault, M.C., Hercberg, S., Riboli, E., Scalbert, A., Siess, M.H., Food and cancer: state of the art about the protective effect of fruits and vegetables, Bull. Cancer 89 (2002) 293–312.
Sasazuki, S., Case-control study of nonfatal myocardial infarction in relation to selected foods in Japanese men and women, Jpn. Circ. J. 65 (2001) 200–206. CrossRef
Bazzano, L.A., He, J., Ogden, L.G., Fruit and vegetable intake and risk of cardiovascular disease in US adults: the first national health and nutrition examination survey epidemiologic follow-up study, Am. J. Clin. Nutr. 76 (2002) 93–99.
Hertog, M.G.L., Feskens, E.J.M., Hollman, P.C.H., Katan, J.B., Kromhout, D., Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen elderly study, Lancet 342 (1993) 1007–1011. CrossRef
Gee, J.M., Johnson, I.T., Polyphenolic compounds: interactions with the gut and implications for human health, Curr. Med. Chem. 8 (2001) 1245–1255. CrossRef
Kahkonen, M.P., Hopia, A.I., Vuorela, H.J., Rauha, J.P., Pihlaja, K., Kujala, T.S., Heinonen, M., Antioxidant activity of plant extracts containing phenolic compounds, J. Agric. Food Chem. 47 (1999) 3954–3962. CrossRef
Scalbert, A., Williamson, G., Dietary intake and bioavailability of polyphenols, J. Nutr. 130 (2000) 2073S–2085S.
Scalbert, A., Morand, C., Manach, C., Rémésy, C., Absorption and metabolism of polyphenols in the gut and impact on health, Biomed. Pharmacother. 56 (2002) 276–282. CrossRef
Sakakibara, H., Honda, Y., Nakagawa, S., Ashida, H., Kanazawa, K. , Simutaneous determination of all polyphenols in vegetables, fruits and teas, J. Agric. Food Chem. 51 (2003) 571–581. CrossRef
Mompon B., Lemaire B., Mengal P., Surbled M., Extraction des polyphénols : du laboratoire à la production industrielle, in : Vercauteren J., Chèze C., Triaud J. (Eds.), Polyphénols 96, Inra, Paris, France, 1998, pp. 31–43.
Bravo, L., Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance, Nutr. Rev. 56 (1998) 317–333. CrossRef
Harborne, J.B., Williams, C.A., Advances in flavonoid research since 1992, Phytochemistry 55 (2000) 481–504. CrossRef
Acworth I.N., Bailey B., The handbook of oxidative metabolism, Ed. ESA Inc, Chelmsford, 1995, 37 p.
Rice-Evans, C.A., Miller, N.J., Paganga, G., Structure-antioxidant activity relationships of flavonoids and phenolic acids, Free Radic. Biol. Med. 20 (1996) 933–956. CrossRef
Silva, F.A.M., Borges, F., Guimaraes, C., Lima, J.L.F.C., Matos, C., Reis, S., Phenolic acids and derivatives: studies on the relationship among structure radical scavenging activity, and physicochemical parameters, J. Agric. Food Chem. 48 (2000) 2122–2126. CrossRef
Lachance, P.A., Nakat, Z., Jeong, W.S., Antioxidants: an integrative approach, Nutrition 17 (2001) 835–838. CrossRef
Fang, Y.Z., Yang, S., Wu, G., Free radicals, antioxidants, and nutrition, Nutrition 18 (2002) 872–879. CrossRefPubMed
Wilson A., Salamatian L., Les radicaux libres : une question d’équilibre, Univ. Versailles/ Saint-Quentin-en-Yvelines, Rapp. DESS IST, Versailles, France, 2002–2003, 37 p.
Salah, N., Miller, N.J., Paganga, G., Tijburg, L., Bolwell, G.P., Rice-Evans, C., Polyphenolic flavanols as scavengers of aqueous phase radicals and as chain-breaking antioxidants, Arch. Biomed. Biophys. 322 (2) (1995) 339–346. CrossRef
Halliwell, B., Antioxidants in human health and disease, Ann. Rev. Nutr. 16 (1996) 33–50. CrossRef
Deng, W., Fang, X., Wu, J., Flavonoids function as antioxidants: by scavenging reactive oxygen species or by chelating iron, Radiat. Phys. Chem. 50 (1997) 271–276. CrossRef
Croft, K.D., The chemistry and biological effects of flavonoids and phenolic acids, Ann. NY Acad. Sci. 854 (1998) 435–442. CrossRef
Geisser P., Iron therapy with special emphasis on oxidative stress, Ed. Vifor (Int.) Inc., Switzerland, 1998, 142 p.
Pierre, J.L., Fontecave, M., Iron and activated oxygen species in biology: the basic chemistry, Biometals 12 (1999) 195–199. CrossRef
Fernandez, M.T., Mira, M.L., Florencio, M.H., Jennings, K.R., Iron and copper chelation by flavonoids: an electrospray mass spectrometry study, J. Inorg. Biochem. 92 (2002) 105–111. CrossRef
Cook, N.C., Samman, S., Flavonoids – chemistry, metabolism, cardioprotective effects, and dietary sources, Nutr. Biochem. 7 (1996) 66–76. CrossRef
Hertog, M.G., Hollman, P.C.H., Katan, M.B., Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9 fruits commonly consumed in the Netherlands, J. Agric. Food Chem. 40 (1992) 2379–2383. CrossRef
Halliwell, B., Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet 344 (1994) 721–724. CrossRef
Mitscher, L.M., Telikepalli, H., McGhee, E., Shankel, D.M., Natural antimutagenic agents, Mutat. Res. 350 (1996) 142–143. CrossRef
Owen, R.W., Giacosa, A., Hull, W.E., Haubner, R., Spiegelhalder, B., Bartsch, H., The antioxidant/anticancer potential of phenolic compounds isolated from olive oil, Eur. J. Cancer 36 (2000) 1235–1247. CrossRef
Sala, A., Recio, M.D., Giner, R.M., Manez, S., Tournier, H., Schinella, G., Rios, J.L., Anti-inflammatory and antioxidant properties of Helichrysum italicum, J. Pharm. Pharmacol. 54 (2002) 365–371. CrossRef
Benavente-Garcia, O., Castillo, J., Marin, F.R., Ortuno, A., Del Rio, J.A., Uses and properties of citrus flavonoids, J. Agric. Food Chem. 45 (1997) 4505–4515. CrossRef
Sánchez-Moreno, C., Plaza, L., De Ancos, B., Pilar Cano, M., Quantitative bioactive compounds assessment and their relative contribution to the antioxidant capacity of commercial orange juices, J. Sci. Food Agric. 83 (2003) 430–439. CrossRef
Malterud, K.E., Rydland, K.M., Inhibitors of 15-lipoxygenase from orange peel, J. Agric. Food Chem. 48 (2000) 5576–5580. CrossRef
Anon., Rapport annuel de la banque centrale de Tunisie (2003), Tunis, Tunisie, 2004, 260 p.
Brand-Williams, W., Cuvelier, M.E., Berset, C., Use of free radical method to evaluate antioxidant activity, Lebensm. Wiss. Technol. 28 (1995) 25–30. CrossRef
Sánchez-Moreno, C., Larrauri, J.A., Saura-Calixto, F., A procedure to measure the antiradical efficiency of polyphenols, J. Sci. Food Agric. 76 (1998) 270–276. 3.0.CO;2-9>CrossRef
Higashi-Okai, K., Kamimoto, K., Yoshioka, A., Okai, Y., Potent suppressive activity of fresh and dried peels from satsuma mandarin Citrus unshiu (Marcov.) on hydroperoxide generation from oxidized linoleic acid, Phytother. Res. 16 (2002) 781–784. CrossRef
Anon., Microcal TM OriginTM, version 5.0, Microcal Software, Inc., Northampton, MA, USA, 1991–1997.