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Is it possible to alter the embryo lipid accumulation with reduction of fetal bovine serum and use of l-carnitine for in vitro maturation of bubaline oocytes?

Published online by Cambridge University Press:  10 January 2020

Marivaldo Rodrigues Figueiró Open the ORCID record for Marivaldo Rodrigues Figueiró [Opens in a new window] ,
Joaquim Mansano Garcia ,
Marina Ragagnin de Lima ,
Maite del Collado  and
Naiara Zoccal Saraiva
Marivaldo Rodrigues Figueiró*
Affiliation:
Embrapa Eastern Amazon, Belém, Pará, Brazil São Paulo State University, School of Agrarian and Veterinary Sciences, Department of Preventive Veterinary Medicine and Animal Reproduction, Jaboticabal, São Paulo, Brazil
Joaquim Mansano Garcia
Affiliation:
São Paulo State University, School of Agrarian and Veterinary Sciences, Department of Preventive Veterinary Medicine and Animal Reproduction, Jaboticabal, São Paulo, Brazil
Marina Ragagnin de Lima
Affiliation:
São Paulo State University, School of Agrarian and Veterinary Sciences, Department of Preventive Veterinary Medicine and Animal Reproduction, Jaboticabal, São Paulo, Brazil
Maite del Collado
Affiliation:
Veterinary Medicine Department, Faculty of Animal Sciences and Food Engineering, University of São Paulo, Pirassununga, São Paulo, Brazil
Naiara Zoccal Saraiva
Affiliation:
Embrapa Dairy Cattle, Juiz de Fora, Minas Gerais, Brazil
*
Author for correspondence: Marivaldo Rodrigues Figueiró. Embrapa Amazônia Oriental, Trav. Dr. Enéas Pinheiro, s/no, Caixa Postal 48, CEP 66095-903, Belém, PA, Brazil. Tel: +55 9132041236. E-mail: marivaldo.figueiro@embrapa.br
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Summary

In vitro embryo production (IVEP) is a procedure that can promote genetic improvement in a short time frame. However, the success rates obtained with this biotechnology in water buffaloes are still inconsistent, and can be associated with the high concentration of lipids in the cytoplasm of oocytes and embryos. The objective of this study was to evaluate the effects of reduced concentration of fetal bovine serum (FBS) and/or use of l-carnitine during in vitro maturation (IVM) on the preimplantation development and lipid accumulation in bubaline embryos. In a first experiment, the lowest concentration of FBS in the IVM medium (0%, 2.5%, 5% or 10%) was determined, and the lowest concentration that maintained good embryo development rates was 5%. In a second experiment, the addition of 5 mM of l-carnitine into the maturation medium was evaluated. The blastocysts produced were submitted to lipid evaluation involving staining followed by observation using optical (Oil Red O) and confocal (BODIPY 493/503) microscopy. No difference was observed between the 5% and 10% FBS groups, which were superior to the 0% and 2.5% groups. Furthermore, the performance of the groups treated with 5% and 10% FBS was better than the groups supplemented with l-carnitine. There was no difference regarding embryo lipid accumulation. The results indicated that it is possible to reduce the FBS concentration to 5% in in vitro maturation medium for production of bubaline embryos, and supplementation with 5 mM l-carnitine does not increase embryo production.

Keywords

BuffaloesEmbryo developmentFetal bovine serumLipid metabolism modulatorNeutral lipids
Type
Research Article
Information
Zygote , Volume 28 , Issue 2 , April 2020 , pp. 109 - 115
Copyright
© Cambridge University Press 2020

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