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All-triploid offspring in the yellowtail tetra Astyanax altiparanae Garutti & Britski 2000 (Teleostei, Characidae) derived from female tetraploid × male diploid crosses

Published online by Cambridge University Press:  09 January 2023

Andreoli Correia Alves Open the ORCID record for Andreoli Correia Alves [Opens in a new window] ,
George Shigueki Yasui ,
Nivaldo Ferreira do Nascimento Open the ORCID record for Nivaldo Ferreira do Nascimento [Opens in a new window] ,
Paulo Sérgio Monzani ,
José Augusto Senhorini Open the ORCID record for José Augusto Senhorini [Opens in a new window]  and
Matheus Pereira dos Santos
Andreoli Correia Alves*
Affiliation:
Federal Rural University of Rio de Janeiro (UFRRJ). Animal Science Graduate Programme, Km 7, Zona Rural, BR-465, s/n, Seropédica RJ Laboratory of Fish Biotechnology, Centro Nacional de Pesquisa e Conservação da Biodiversidade Aquática Continental CEPTA/ICMbio, Rodovia Pref. Euberto Nemésio Pereira de Godoy, Pirassununga, SP 13630–970, Brazil
George Shigueki Yasui
Affiliation:
Laboratory of Fish Biotechnology, Centro Nacional de Pesquisa e Conservação da Biodiversidade Aquática Continental CEPTA/ICMbio, Rodovia Pref. Euberto Nemésio Pereira de Godoy, Pirassununga, SP 13630–970, Brazil University of São Paulo, School of Veterinary Medicine and Animal Science, Department of Animal Reproduction, São Paulo, Brazil
Nivaldo Ferreira do Nascimento
Affiliation:
Laboratory of Fish Biotechnology, Centro Nacional de Pesquisa e Conservação da Biodiversidade Aquática Continental CEPTA/ICMbio, Rodovia Pref. Euberto Nemésio Pereira de Godoy, Pirassununga, SP 13630–970, Brazil Federal Rural University of Pernambuco (UFRPE), Unidade Acadêmica de Serra Talhada, Serra Talhada, Av. Gregório Ferraz Nogueira, s/n, Serra Talhada PE
Paulo Sérgio Monzani
Affiliation:
Laboratory of Fish Biotechnology, Centro Nacional de Pesquisa e Conservação da Biodiversidade Aquática Continental CEPTA/ICMbio, Rodovia Pref. Euberto Nemésio Pereira de Godoy, Pirassununga, SP 13630–970, Brazil Paulista State University (UNESP), Department of Zoology, Botucatu, SP, Brazil
José Augusto Senhorini
Affiliation:
Laboratory of Fish Biotechnology, Centro Nacional de Pesquisa e Conservação da Biodiversidade Aquática Continental CEPTA/ICMbio, Rodovia Pref. Euberto Nemésio Pereira de Godoy, Pirassununga, SP 13630–970, Brazil Paulista State University (UNESP), Department of Zoology, Botucatu, SP, Brazil
Matheus Pereira dos Santos
Affiliation:
Federal Rural University of Rio de Janeiro (UFRRJ). Animal Science Graduate Programme, Km 7, Zona Rural, BR-465, s/n, Seropédica RJ
*
Author for correspondence: Andreoli Correia Alves. Federal Rural University of Rio de Janeiro (UFRRJ). Animal Science Graduate Programme, Km 7, Zona Rural, BR-465, s/n, Seropédica RJ, Brazil. E-mail: andreoliaca@gmail.com
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Summary

This study aimed to evaluate the ploidy and survival of larvae resulting from crosses between tetraploid females and diploid males of yellowtail tetra Astyanax altiparanae, both females (three diploids and three tetraploids) and males (n = 3 diploids). Breeders were subjected to hormonal induction with pituitary gland extract from common carp fish (Cyprinus carpio). Females received two doses at concentrations of 0.3 and 3.0 mg/kg −1 body weight and at intervals of 6 h. Males were induced with a single dose of 3.0 mg/kg −1 applied simultaneously with the second dose in females. Oocytes from each diploid and tetraploid female were fertilized with semen from the same male, resulting in two crosses: cross 1 (diploid male and diploid female) and cross 2 (diploid male and tetraploid female). The procedures were performed with separate females (diploid and tetraploid) and diploid males for each repetition (n = 3). For ploidy determination, 60 larvae from each treatment were analyzed using flow cytometry and cytogenetic analyses. As expected, flow cytometry analysis showed that progenies from crosses 1 and 2 presented diploid and triploid individuals, respectively, with a 100% success rate. The same results were confirmed in the cytogenetic analysis, in which the larvae resulting from cross 1 had 50 metaphase chromosomes and those from cross 2 had 75 chromosomes. The oocytes have a slightly ovoid shape at the time of extrusion. Diploid oocytes had a size of 559 ± 20.62 μm and tetraploid of 1025.33 ± 30.91 μm. Statistical differences were observed between eggs from crosses 1 and 2 (P = 0.0130). No significant differences between treatments were observed for survival at the 2-cell stage (P = 0.6174), blastula (P = 0.9717), gastrula (P = 0.5301), somite (P = 0.3811), and hatching (P = 0.0984) stages. In conclusion, our results showed that tetraploid females of the yellowtail tetra A. altiparanae are fertile, present viable gametes after stripping and fertilization using the ‘dry method’, and may be used for mass production of triploids. This is the first report of these procedures within neotropical characins, and which can be applied in other related species of economic importance.

Keywords

AquacultureChromosome manipulationEmbryologyLambariTriploids mass production
Type
Research Article
Information
Zygote , Volume 31 , Issue 2 , April 2023 , pp. 123 - 128
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Copyright
© The Author(s), 2023. Published by Cambridge University Press

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