The sex workers of Venice, and especially its high-end courtesans, were famous in the Renaissance and have continued ever since to attract the attention of scholars. Taking a novel approach to sex workers as a group, my larger project uses mapping to situate their activities and movements within Venice's unique urban geography. This article draws on a combination of sources from the fifteenth and sixteenth centuries to reconstruct how space framed interactions between these women and other urban dwellers. Although contemporaries deemed sex workers ubiquitous in the city, some locations and pathways, where they habitually encountered clients and other Venetians, became associated with the trade. These include not only the two official brothels, but also sex workers’ private dwellings and other sites in zones where complementary businesses, low rents, and intersections of circulation networks of various scales served their needs. Examples from the parishes of San Cassiano, Santa Maria Formosa, and San Giovanni Novo, and the district of Cannaregio show that sex work was a highly social, publicly visible business and that the city served as the sex worker's workplace. Alleys, quays, bridges, porticos, and peripheral squares anchored her interactions with procurers and, more often, procuresses, as well as servants, lodging house matrons, bathhouse attendants, tavern and inn keepers, porters, gondoliers, clients, and others who moved in and out of her orbit. The places where they met, generally overlooked by architectural and urban histories that privilege the monumental over the vernacular, produced a spatial syntax of sex work that integrated the trade into everyday neighborhood life.