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Activation of GABAA receptors in the medial prefrontal cortex produces an anxiolytic-like response

Published online by Cambridge University Press:  08 March 2013

Jalal Solati ,
Ramin Hajikhani  and
Yulia Golub
Jalal Solati
Affiliation:
Department of Biology, Karaj branch, Islamic Azad University, Karaj, Iran
Ramin Hajikhani
Affiliation:
Department of Biology, Karaj branch, Islamic Azad University, Karaj, Iran
Yulia Golub*
Affiliation:
Department of Child and Adolescent Mental Health, University of Erlangen-Nuremberg
*
Jalal Solati, Department of Biology, School of Science, Karaj Branch, Islamic Azad University, Karaj, Iran. Tel: +98261 4418143; Fax: +98261 4418156; E-mail solati@kiau.ac.ir;
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Abstract

Objectives

There has been increasing evidence that the γ-aminobutyric acid (GABA)ergic system is involved in the neurobiology of anxiety. The present study aimed to investigate the role of GABAergic systems in the modulation of anxiety in the medial prefrontal cortex (mPFC) of rats using the elevated plus maze test.

Methods

Rats were anaesthetised with a mixture of ketamine and xylazine, and then special cannulae were inserted stereotaxically into the mPFC. After 5–7 days of recovery, the effects of intra-mPFC administration of GABAergic agents were studied.

Results

Bilateral injection of the GABAA receptor agonist muscimol (0.25, 0.5 and 1 μg/rat) produces an anxiolytic-like effect, shown by significant increases in the percentage of open-arm time (%OAT) and percentage of open-arm entries (%OAE). Intra-mPFC administration of the GABAA receptor antagonist bicuculline (0.25, 0.5 and 1 μg/rat) produces significant anxiogenic-like behaviour. However, intra-mPFC injection of the GABAB receptor agonist baclofen (0.05, 0.1 and 0.2 μg/rat) and the GABAB receptor antagonist CGP35348 (5, 10 and 15 μg/rat) did not alter %OAT and %OAE significantly.

Conclusion

The results of the present study demonstrate that the GABAergic system of the mPFC modulates anxiety-related behaviours of rats through GABAA receptors.

Keywords

anxietyGABAmedial prefrontal cortex
Type
Original Articles
Information
Acta Neuropsychiatrica , Volume 25 , Issue 4 , August 2013 , pp. 221 - 226
Copyright
Copyright © Scandinavian College of Neuropsychopharmacology 2013 

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