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Protective effect of dl-3n-butylphthalide preconditioning on focal cerebral ischaemia-reperfusion injury in rats

Published online by Cambridge University Press:  22 February 2013

Pei-Lei Zhang ,
Hai-Tao Lu ,
Jun-Gong Zhao  and
Ming-Hua Li
Pei-Lei Zhang
Affiliation:
Department of Diagnostic and Interventinal Radiology, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, People's Republic of China
Hai-Tao Lu*
Affiliation:
Department of Diagnostic and Interventinal Radiology, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, People's Republic of China
Jun-Gong Zhao*
Affiliation:
Department of Diagnostic and Interventinal Radiology, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, People's Republic of China
Ming-Hua Li
Affiliation:
Department of Diagnostic and Interventinal Radiology, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, People's Republic of China
*
Hai-Tao Lu and Jun-Gong Zhao, Department of Diagnostic and Interventinal Radiololgy, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, 600 Yi Shan Rd, Shanghai 200233, People's Republic of China. Tel: +862164369181 (ext. 8016); Fax: +862164844183; E-mail: luhaitao1975@hotmail.com; zhaojungong11@yahoo.cn
Hai-Tao Lu and Jun-Gong Zhao, Department of Diagnostic and Interventinal Radiololgy, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, 600 Yi Shan Rd, Shanghai 200233, People's Republic of China. Tel: +862164369181 (ext. 8016); Fax: +862164844183; E-mail: luhaitao1975@hotmail.com; zhaojungong11@yahoo.cn
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Abstract

Objective

To investigate the effect of dl-3n-butylphthalide (NBP) on the protection of cerebral tissue and possible mechanism on ischaemia-reperfusion injury, and to find out whether NBP therapy can extend the reperfusion window in an experimental stroke model in rats.

Methods

Seventy-two Sprague-Dawley rats were randomly divided into sham operation, ischaemia-reperfusion and ischaemia-reperfusion with NBP groups. Focal cerebral ischaemia was induced using the modified intraluminal thread method and maintained for 2, 3 or 4 h. The ischaemia-reperfusion group received reperfusion immediately after ischaemia-reperfusion. The NBP group received intraperitoneal injection of NBP immediately after ischaemia, followed by reperfusion. The sham operation group received only injection of physiological saline. The cerebral infarction volume and neurological deficit were analysed, and vascular endothelial growth factor (VEGF) expression in brain tissues was visualised by immunohistochemistry.

Results

NBP treatment caused a significant decrease in both infarction volume and neurological deficit compared with the ischaemia-reperfusion group at corresponding time points in each (p < 0.05). In the NBP group, the infarction volume and neurological deficit did not change with different ischaemia times. The expression of VEGF was significantly decreased in the ischaemia-reperfusion group compared with the sham group (p < 0.01), while this change was partly prevented in the NBP group (p < 0.01). The expression of VEGF in brain tissue in both the NBP and ischaemia-reperfusion groups gradually decreased when the ischaemic period was prolonged.

Conclusion

NBP treatment has a protective effect against cerebral ischaemia; this possible mechanism maybe related to the VEGF expression and may extend the reperfusion window for subsequent salvage of cerebral ischaemia by reperfusion.

Keywords

cerebral ischaemiadl-3n-butylphthalideneuroprotectionratsreperfusion injuryvascular endothelial growth factor
Type
Original Articles
Information
Acta Neuropsychiatrica , Volume 25 , Issue 1 , February 2013 , pp. 12 - 17
Copyright
Scandinavian College of Neuropsychopharmacology 2013

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