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Differential Acridine Orange Staining of Human Chromosomes

Published online by Cambridge University Press:  01 August 2014

G.L. Castoldi ,
G.D. Grusovin ,
G.L. Scapoli  and
R. Spanedda
G.L. Castoldi*
Affiliation:
Medical Clinic of the University, Ferrara, Italy
G.D. Grusovin
Affiliation:
Medical Clinic of the University, Ferrara, Italy
G.L. Scapoli
Affiliation:
Medical Clinic of the University, Ferrara, Italy
R. Spanedda
Affiliation:
Medical Clinic of the University, Ferrara, Italy
*
Clinica Medica dell'Università, Ferrara, Italy

Summary

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The acridine orange staining of metaphases previously treated with hot salt solutions, exhibits a differential banding pattern of the chromosomes. According to the physicochemical interpretation of the stained structures, the green and red fluorescent segments of the chromosomes should be considered as constituted respectively by double-stranded DNA and single-stranded DNA. The banding pattern is relatively consistent in different metaphases, although some occasional variations of the bands may be referred to the interference of chromosomal acid proteins. In general, the sequence of the bands is compatible with the picture of the reverse banding.

Type
Research Article
Information
Acta geneticae medicae et gemellologiae: twin research , Volume 21 , Issue 4 , October 1972 , pp. 319 - 326
Copyright
Copyright © The International Society for Twin Studies 1978

References

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