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Polymorphism in exon 2 of the BuLA-DRB3 gene in Indian buffalo (Bubalus bubalis var. indicus) detected by PCR-RFLP

Published online by Cambridge University Press:  18 August 2016

T.V. Aravindakshan ,
A.M. Nainar  and
S.N. Sivaselvam
T.V. Aravindakshan
Affiliation:
Department of Animal Biotechnology, Tamil Nadu Veterinary and Animal Sciences University, Chennai 600 007, Tamil Nadu, India
A.M. Nainar
Affiliation:
Department of Animal Biotechnology, Tamil Nadu Veterinary and Animal Sciences University, Chennai 600 007, Tamil Nadu, India
S.N. Sivaselvam
Affiliation:
Department of Animal Genetics, Tamil Nadu Veterinary and Animal Sciences University, Chennai 600 007, Tamil Nadu, India
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Abstract

Polymerase chain reaction (PCR) primers specific to exon 2 of the bovine lymphocyte antigen (BoLA)-DRB3 gene were used successfully to amplify the equivalent region in 34 Murrah and 36 Surti buffaloes selected at random. The 304 bp amplified product of the DRB3 gene was separately digested with BstγI, HaeIII and Rsal enzymes. Digestion with BstγI enzyme did not reveal any polymorphism and all animals showed a single restriction pattern, which corresponded exactly to the BstγI pattern ‘b’ previously described for cattle. Digestion with HaeIII enzyme resulted in five patterns, four of which corresponded to the Haelll patterns previously reported in cattle. The new HaeIII pattern was observed in both the breeds of buffaloes studied. The fragment analysis with RsaI revealed 13 different patterns. All of these RsaI patterns corresponded to the RsaI patterns previously described for cattle. The high degree of similarity in the restriction fragment length polymorphism (RFLP) patterns of cattle and buffalo observed in the present study provide evidence for the strong conservation amongst other bovine species, of restriction sites previously reported in cattle.

Keywords

buffaloesgenetic polymorphismpolymerase chain reactionrestriction fragment length polymorphism
Type
Breeding and genetics
Information
Animal Science , Volume 70 , Issue 2 , April 2000 , pp. 221 - 226
Copyright
Copyright © British Society of Animal Science 2000

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