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Renal and salivary clearance of purine derivatives in sheep

Published online by Cambridge University Press:  02 September 2010

J. C. Surra ,
J. A. Guada ,
J. Balcells  and
C. Castrillo
J. C. Surra
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet, 177, 50013 Zaragoza, Spain
J. A. Guada
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet, 177, 50013 Zaragoza, Spain
J. Balcells
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet, 177, 50013 Zaragoza, Spain
C. Castrillo
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet, 177, 50013 Zaragoza, Spain
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Abstract

Four adult ewes (mean weight 42·6 kg) fitted with oesophageal fistulae were given 5 mmol/day ofallantoin or saline solutions by intrajugular continuous infusion. The experiment was a randomized cross-over design, with two consecutive 3-day infusion periods. One kg/day fresh matter of either chopped or pelleted fescue hay was distributed over 12 meals and salivary flow estimated from dilution of Co-EDTA infused into the buccal cavity. Allantoin infusion resulted in a rapid increase in its plasma concentration (84 to 128 (s.e. 1·5) μmol/l) and urinary excretion (9·6 to 13·3 (s.e. 0·18) mmol/day) without significant differences between diets. Salivary allantoin also increased (4·6 to 6·4 (s.e. 0·60) ymol/1) in response to infusion, although the concentration of total purine derivatives in saliva was only proportionately 0·08 that of plasma. Renal and salivary clearance of oxypurines, allantoin (78 (s.e. 5·0) ml/min and 13 (s.e. 0·7) ml/h), uric acid (466 (s.e. 98·0) ml/min and 45 (s.e. 9·8) ml/h) and creatinine (104 (s.e. 3·0) ml/min and 14 (s.e. 1·1) ml/h) were constant, irrespective of diet and infusion treatments. Urinary recovery of infused allantoin averaged 0·78 (s.e. 0·031) but salivary secretion, equivalent to about 0·003 of urinary losses, was not the explanation for the incomplete recovery.

Keywords

purinessalivasheep
Type
Research Article
Information
Animal Science , Volume 65 , Issue 1 , August 1997 , pp. 83 - 91
Copyright
Copyright © British Society of Animal Science 1997

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