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A long-time ambient temperature diluent for boar semen

Published online by Cambridge University Press:  02 September 2010

S. G. Revell  and
C. E. Glossop
S. G. Revell
Affiliation:
MMB Freezing Unit, Llanrhydd, Ruthin, Clwyd, LL15 2 UP
C. E. Glossop
Affiliation:
MAFF Cattle Breeding Centre, Church Lane, Shinfield, Reading RG2 9BZ
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Abstract

Semen was collected from boars of proven fertility and diluted with Zorpva base using the antibiotic treatments: benzylpenicillin and streptomycin; polymixin B; tylosin; neomycin; gentamycin; kanamycin or lincomycin and spectinomycin. The last combination provided an economical treatment with enhanced quality and preservation of motility. The addition of trehalose (1 g/1) and potassium chloride (0·25 g/1) improved the preservation of acrosomes. The resulting ‘Reading’ variant was compared with Zorpva at days 4 to 6 of storage in a split ejaculate trial using 40 sows slaughtered 9/10 weeks after insemination. Conception rates were significantly higher using Reading (16/20) compared with Zorpva (8/20; P < 0·05) and the effect was particularly marked at days 5 and 6. The mean number of embryos per pregnant sow was 11·6 (Reading) v. 9·4 (Zorpva), representing 51% and 42%, respectively, of ova shed, as measured by counting corpora lutea.

Type
Papers
Information
Animal Production , Volume 48 , Issue 3 , June 1989 , pp. 579 - 584
Copyright
Copyright © British Society of Animal Science 1989

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References

REFERENCES

Aalbers, J. G. and Smidt, W. J. 1984. Use of semenmore than 24 hours old in pig AI. Tijdschrift voor Diergeneeskunde 109: (9), 361.Google Scholar
Almlid, T., Blitchfeldt, T. and Stavne, S. E. 1984. Trials with different diluting fluids for boar AI semen. 10th International Congress on Animal Reproduction and Artificial Insemination, Urbana, Illinois, Vol. II, Paper 182.Google Scholar
Alvarez, J. G. and Storey, B. T. 1982. Spontaneous lipid peroxidation in rabbit epididymal spermatozoa: its effect on sperm motility. Biology of Reproduction 27: 1102–1108.CrossRefGoogle ScholarPubMed
Brander, G. C. and Pugh, D. M. 1971. Veterinary Applied Pharmacology and Therapeutics. 2nd ed,. pp. 310–311, Bailliere Tindall, London.Google Scholar
Brunel, L., Gottardi, L. and Zanelli, I. 1981. [New diluent for pig semen.] Informatore Zootechnico 27: (11), 51–53.Google Scholar
Cheng, T. T.-K. 1985. In vitro fertilisation of farm animal oocytes. Ph.D. Thesis, Council for National Academic Awards, UK.Google Scholar
Gottardi, L., Brunel, L. and Zanelli, L. 1980. New dilution media for artificial insemination in pigs. IXth International Congress on Animal Reproduction and Artificial Insemination, Madrid, Vol. V, pp. 49–53.Google Scholar
Herak, M., Herak, M., Branezac, N., Premzl, B. and Sviben, M. 1984. Zorlesco diluting agent: possibility of application in dilution of ejaculates and artificial insemination of sows with dosages of sperm kept for 5 days. Stocarstvo 38: 9-10; 309–313.Google Scholar
Martin Rillo, S., Sebastian, J. J., Alias, E. and Diaz Yubero, C. 1984. The effect of antibiotic associations in the conservation of boar semen at 15°C. Proceedings of the International Pig Veterinary Society Congress, Ghent, Belgium, p. 295.Google Scholar
Meding, J. H. 1982. Sammenligning af befruchtningseffertiviteten af ornesaed fortyndet med EDTA og ‘Zorlesco’, med henblik pa udstraekning af saedens anvenvelsestid. Meddelelse, Statens Husdyrbrugsforog, 409.Google Scholar
Paouignon, M. 1984. Semen technology in the pig. In The Male in Farm Animal Reproduction, pp. 202–218. Martinus Nijhoff, Boston.Google Scholar
Rudolph, A. S. and Crowe, J. H. 1985. Membrane stabilization during freezing: the role of two natural cryoprotectants, trehalose and proline. Cryobiology 22: 367–377.CrossRefGoogle ScholarPubMed
Sone, M. 1982. Effects of various antibiotics on the control of bacteria in boar semen. Veterinary Record 111: 11–14.CrossRefGoogle ScholarPubMed
Tamuli, M. K., Sharma, D. K. and Rajkonwar, C. K. 1984. Studies on the microbial flora of boar semen. Indian Veterinary Journal 61: 858–861.Google Scholar
Wooley, R. E., Jones, M. S. and Shotts, E. B. 1984. Uptake of antibiotics in gram-negative bacteria exposed to EDTA-TRIS. Veterinary Microbiology 10: 57–70.CrossRefGoogle ScholarPubMed