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Effect of vitamin A supplementation at different gaseous environments on in vitro development of pre-implantation sheep embryos to the blastocyst stage

Published online by Cambridge University Press:  08 June 2010

N. Rajesh ,
M. B. Shankar  and
M. Deecaraman
N. Rajesh*
Affiliation:
Dr MGR Educational and Research Institute, Dr MGR University, Maduravoyal, Chennai – 600095, Tamilnadu, India
M. B. Shankar
Affiliation:
Quality Assurance Department, Huclin Research Limited, TICEL Biopark, Taramani, Chennai-600113, Tamilnadu, India
M. Deecaraman
Affiliation:
Dr MGR Educational and Research Institute, Dr MGR University, Maduravoyal, Chennai – 600095, Tamilnadu, India
*
†E-mail: r_jesh2002@yahoo.com
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Abstract

Vitamin A (all-trans retinol) is an important antioxidant whose role in embryo development in vitro and in vivo is well established. Oxidative stress is a major cause of defective embryo development. This study evaluated the effects of all-trans retinol supplementation to maturation and embryo culture media under different gaseous environments on the development of ovine oocytes and embryos in vitro. The percentages of cleavage, morula and blastocyst, total cell count and comet assay were taken as indicators of developmental competence of embryos. In experiments I and II, all-trans retinol at concentrations of 0, 2, 4, 6, 8 and 10 μM were supplemented to the oocyte maturation medium and cultured in an environment of 5% or 20% O2 respectively. All-trans retinol supplementation (6 μM) to the maturation medium at 5% O2 levels significantly increased blastocyst yield and total cell number (P < 0.05). Maturation of oocytes in a 20% O2 environment bettered cleavage rates in the 6 μM supplemented group compared with the control group (P < 0.05). In experiments III and IV, all-trans retinol, at the aforesaid concentrations was supplemented to embryo culture media under a 5% or 20% O2 environment, respectively. All-trans retinol supplementation to the embryo culture medium at 5% O2 levels did not yield any significant result whereas the culture at 20% O2 levels gave significantly higher blastocyst yield in the 6 μM supplemented group compared with the control group (P < 0.01).

Keywords

vitamin Aovinein vitro fertilisationoxidative stressembryo culture
Type
Full Paper
Information
animal , Volume 4 , Issue 11 , November 2010 , pp. 1884 - 1890
Copyright
Copyright © The Animal Consortium 2010

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