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Effects of spermine on the antioxidant status and gene expression of antioxidant-related signaling molecules in the liver and longissimus dorsi of piglets

Published online by Cambridge University Press:  25 October 2017

T. Fang ,
J. Zheng ,
W. Cao ,
G. Jia ,
H. Zhao ,
X. Chen ,
J. Cai ,
J. Wang  and
G. Liu
T. Fang
Affiliation:
Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, China Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu 611130, Sichuan, China
J. Zheng
Affiliation:
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
W. Cao
Affiliation:
Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, China Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu 611130, Sichuan, China
G. Jia
Affiliation:
Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, China Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu 611130, Sichuan, China
H. Zhao
Affiliation:
Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, China Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu 611130, Sichuan, China
X. Chen
Affiliation:
Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, China Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu 611130, Sichuan, China
J. Cai
Affiliation:
Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, China Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu 611130, Sichuan, China
J. Wang
Affiliation:
Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
G. Liu*
Affiliation:
Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, China Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu 611130, Sichuan, China
*
†E-mail: liugm@sicau.edu.cn
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Abstract

Previous studies showed that spermine could protect the organism from oxidative damage in vivo. However, in vivo information on the antioxidant-related underlying molecular mechanism of spermine is limited. In this experiment, we further evaluated the effects of spermine supplementation and extended spermine administration on the antioxidant status and antioxidant-related signaling molecules gene expression in the liver and longissimus dorsi of piglets. A total of 80 piglets were randomly distributed to two groups, that is, those with adequate nutrient intake administrated with spermine (0.4 mmol/kg BW) or those with restricted nutrient intake supplemented by saline. The piglets were fed in pairs for 7 h or 3, 6, or 9 days. The results are as follows: (1) spermine can promote the antioxidant capacity by increasing enzymatic antioxidant capacity, glutathione content and clearance of oxygen radicals; (2) spermine significantly increased the mRNA levels of enzymatic antioxidant substances, NF-E2-related nuclear factor 2, Kelch-like ECH-associated protein 1, and the mammalian target of rapamycin but decreased the mRNA levels of ribosomal p70 S6 kinase in the liver and longissimus dorsi of the piglets.

Keywords

spermineantioxidant statusliverlongissimus dorsipiglets
Type
Research Article
Information
animal , Volume 12 , Issue 6 , June 2018 , pp. 1208 - 1216
Copyright
© The Animal Consortium 2017 

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Footnotes

a

These authors contributed equally to this study.

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