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Bovine mtDNA D-loop haplotypes exceed mutations in number despite reduced recombination: an effective alternative for identity control

Published online by Cambridge University Press:  07 June 2010

E. Seroussi  and
E. Yakobson
E. Seroussi*
Affiliation:
Agricultural Research Organization, Institute of Animal Sciences, Bet-Dagan 50250, Israel
E. Yakobson
Affiliation:
Agricultural Research Organization, Institute of Animal Sciences, Bet-Dagan 50250, Israel
*
†E-mail: seroussi@agri.huji.ac.il
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Abstract

Mitochondrial (mt) DNA D-loop heterogeneity, haplotype distribution and possible sub-population structures within the relevant populations are important for DNA-based traceability. To gain insight into this distribution, we compared 1515 Bos taurus mtDNA D-loop sequences available from GenBank to 219 sequences that we sequenced de novo. A pronounced ambiguous trace typical of C-track length heteroplasmy was encountered in 5% of the samples, which were excluded from the analysis. Previously undescribed mutations and haplotypes were observed in 6% and 63% of the sequences, respectively. B. taurus haplotypes divided into the taurus, indicus and grunniens types and 302 variable sites formed the 858 taurus haplotypes detected. Fifty-five sites displayed a complex level of variation. As each level represents an independent mutation event, a total of 399 mutations were traced, which could potentially explain independent formation of less than half (47%) of the haplotypes encountered: most haplotypes were derived from different combinations of these mutations. We suggest that a mutational hotspot may explain these results and discuss the usefulness of mtDNA for identity and maternity assurance.

Keywords

traceabilityDNA recombinationheteroplasmymitochondrionD-loop
Type
Full Paper
Information
animal , Volume 4 , Issue 11 , November 2010 , pp. 1818 - 1822
Copyright
Copyright © The Animal Consortium 2010

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