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Inheritance of porcine receptors for enterotoxigenic Escherichia coli with fimbriae F4ad and their relation to other F4 receptors

Published online by Cambridge University Press:  11 April 2014

A. Rampoldi ,
H. U. Bertschinger ,
E. Bürgi ,
G. Dolf ,
X. Sidler ,
A. Bratus ,
P. Vögeli  and
S. Neuenschwander
A. Rampoldi
Affiliation:
Institute of Agricultural Sciences, ETH Zurich, 8092 Zurich, Switzerland
H. U. Bertschinger
Affiliation:
Institute of Agricultural Sciences, ETH Zurich, 8092 Zurich, Switzerland
E. Bürgi
Affiliation:
Department of Farm Animals, Vetsuisse Faculty, University of Zurich, 8057 Zurich, Switzerland
G. Dolf
Affiliation:
Institute of Genetics, Vetsuisse Faculty, University of Berne, 3012 Berne, Switzerland
X. Sidler
Affiliation:
Department of Farm Animals, Vetsuisse Faculty, University of Zurich, 8057 Zurich, Switzerland
A. Bratus
Affiliation:
Institute of Agricultural Sciences, ETH Zurich, 8092 Zurich, Switzerland
P. Vögeli
Affiliation:
Institute of Agricultural Sciences, ETH Zurich, 8092 Zurich, Switzerland
S. Neuenschwander*
Affiliation:
Institute of Agricultural Sciences, ETH Zurich, 8092 Zurich, Switzerland
*
†E-mail: stefan.neuenschwander@usys.ethz.ch
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Abstract

Enteric Escherichia coli infections are a highly relevant cause of disease and death in young pigs. Breeding genetically resistant pigs is an economical and sustainable method of prevention. Resistant pigs are protected against colonization of the intestine through the absence of receptors for the bacterial fimbriae, which mediate adhesion to the intestinal surface. The present work aimed at elucidation of the mode of inheritance of the F4ad receptor which according to former investigations appeared quite confusing. Intestines of 489 pigs of an experimental herd were examined by a microscopic adhesion test modified in such a manner that four small intestinal sites instead of one were tested for adhesion of the fimbrial variant F4ad. Segregation analysis revealed that the mixed inheritance model explained our data best. The heritability of the F4ad phenotype was estimated to be 0.7±0.1. There are no relations to the strong receptors for variants F4ab and F4ac. Targeted matings allowed the discrimination between two F4ad receptors, that is, a fully adhesive receptor (F4adRFA) expressed on all enterocytes and at all small intestinal sites, and a partially adhesive receptor (F4adRPA) variably expressed at different sites and often leading to partial bacterial adhesion. In pigs with both F4ad receptors, the F4adRPA receptor is masked by the F4adRFA. The hypothesis that F4adRFA must be encoded by at least two complementary or epistatic dominant genes is supported by the Hardy–Weinberg equilibrium statistics. The F4adRPA receptor is inherited as a monogenetic dominant trait. A comparable partially adhesive receptor for variant F4ab (F4abRPA) was also observed but the limited data did not allow a prediction of the mode of inheritance. Pigs were therefore classified into one of eight receptor phenotypes: A1 (F4abRFA/F4acR+/F4adRFA); A2 (F4abRFA/F4acR+/F4adRPA); B (F4abRFA/F4acR+/F4adR−); C1 (F4abRPA/F4acR−/F4adRFA); C2 (F4abRPA/F4acR−/F4adRPA); D1 (F4abR−/F4acR−/F4adRFA); D2 (F4abR−/F4acR−/F4adRPA); E (F4abR−/F4acR−/F4adR−).

Keywords

enterotoxigenic E. colifully adhesivepartially adhesiveinheritance of F4ad receptorspig
Type
Full Paper
Information
animal , Volume 8 , Issue 6 , June 2014 , pp. 859 - 866
Copyright
© The Animal Consortium 2014 

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References

Alexa, P, Štouračová, K, Hamřík, J and Rychlík, I 2001. Gene typing of the colonisation factors K88 (F4) in the enterotoxigenic Escherichia coli strains isolated from diarrhoeic piglets. Veterinární Medicína 46, 46–49.CrossRefGoogle Scholar
Bijlsma, IG and Bouw, J 1987. Inheritance of K88-mediated adhesion of Escherichia coli to jejunal brush borders in pigs: a genetic analysis. Veterinary Research Communications 11, 509–518.Google Scholar
Bijlsma, IG, de Nijs, A, van der Meer, C and Frik, JF 1982. Different pig phenotypes affect adherence of Escherichia coli to jejunal brush borders by K88ab, K88ac, or K88ad antigen. Infection and Immunity 37, 891–894.Google Scholar
Billey, LO, Erickson, AK and Francis, DH 1998. Multiple receptors on porcine intestinal epithelial cells for the three variants of Escherichia coli K88 fimbrial adhesin. Veterinary Microbiology 59, 203–212.Google Scholar
Boldman, KG, Kriese, LA, van Vleck, LD and Kachman, SD 1995. A manual for use of MTDFREML – a set of programs to obtain estimates of variances and covariances. US Department of Agriculture, Agricultural Research Service, University of Nebraska, Lincoln, NE, USA. ftp://aipl.arsusda.gov/pub/outgoing/mtdfreml/mtdfrman.pdf Google Scholar
Francis, DH, Erickson, AK and Grange, PA 1999. K88 adhesins of enterotoxigenic Escherichia coli and their porcine enterocyte receptors. Advances in Experimental Medicine and Biology 473, 147–154.CrossRefGoogle ScholarPubMed
Gill, PE, Murray, W, Saunders, MA and Wright, MH 1984. User’s guide for NPSOL (version 2.1): A Fortran package for nonlinear programming. Systems Optimization Laboratory, Department of Operations Research, Stanford University, Stanford, CA, USA.Google Scholar
Guinée, PA and Jansen, WH 1979. Behavior of Escherichia coli K antigens K88ab, K88ac, and K88ad in immunoelectrophoresis, double diffusion, and hemagglutination. Infection and Immunity 23, 700–705.Google Scholar
Hasstedt, SJ 2002. PAP: pedigree analysis package, version 5.0. Department of Human Genetics, University of Utah, Salt Lake City, UT, USA.Google Scholar
Hu, ZL, Hasler-Rapacz, J, Huang, SC and Rapacz, J 1993. Studies in swine on inheritance and variation in expression of small intestinal receptors mediating adhesion of the K88 enteropathogenic Escherichia coli variants. Journal of Heredity 84, 157–165.Google Scholar
Jensen, VF, Eborg, H-D and Aarestrup, FM 2011. Indications and patterns of therapeutic use of antimicrobial agents in the Danish pig production from 2002 to 2008. Journal of Veterinary Pharmacology and Therapeutics 35, 33–46.Google Scholar
Melkebeek, V, Goddeeris, BM and Cox, E 2013. ETEC vaccination in pigs. Veterinary Immunology and Immunopathology 152, 37–42.Google Scholar
Python, P, Jörg, H, Neuenschwander, S, Hagger, C, Stricker, C, Bürgi, E, Bertschinger, HU, Stranzinger, G and Vögeli, P 2002. Fine-mapping of the intestinal receptor locus for enterotoxigenic Escherichia coli F4ac on porcine chromosome 13. Animal Genetics 33, 441–447.CrossRefGoogle ScholarPubMed
Python, P, Jörg, H, Neuenschwander, S, Asai-Coakwell, M, Hagger, C, Bürgi, E, Bertschinger, HU, Stranzinger, G and Vögeli, P 2005. Inheritance of the F4ab, F4ac and F4ad E. coli receptors in swine and examination of four candidate genes for F4acR. Journal of Animal Breeding and Genetics 122 (suppl. 1), 5–14.Google Scholar
Ren, J, Yan, X, Ai, H, Zhang, Z, Huang, X, Ouyang, J, Yang, M, Yang, H, Han, P, Zeng, W, Chen, Y, Guo, Y, Xiao, S, Ding, N and Huang, L 2012. Susceptibility towards enterotoxigenic Escherichia coli F4ac diarrhea is governed by the MUC13 gene in pigs. PLos One 7, e44573.Google Scholar
Schroyen, M, Stinckens, A, Verhelst, R, Niewold, T and Buys, N 2012. The search for the gene mutations underlying enterotoxigenic Escherichia coli F4ab/ac susceptibility in pigs: a review. Veterinary Research 43, 70.Google Scholar
Thorns, CJ, Boarer, CDH and Morris, JA 1987. Production and evaluation of monoclonal antibodies directed against the fimbrial adhesins produced by Escherichia coli enterotoxigenic for piglets. Research in Veterinary Science 43, 233–238.CrossRefGoogle ScholarPubMed
Vögeli, P, Bertschinger, HU, Stamm, M, Stricker, C, Hagger, C, Fries, R, Rapacz, J and Stranzinger, G 1996. Genes specifying receptors for F18 fimbriated Escherichia coli, causing oedema disease and postweaning diarrhoea in pigs, map to chromosome 6. Animal Genetics 27, 321–328.Google ScholarPubMed
Wang, J, Jiang, SW, Chen, XH, Liu, ZL and Peng, J 2006. Prevalence of fimbrial antigen (K88 variants, K99 and 987P) of enterotoxigenic Escherichia coli from neonatal and post-weaning piglets with diarrhea in central China. Asian-Australasian Journal of Animal Sciences 19, 1342–1346.CrossRefGoogle Scholar
Yan, X, Huang, X, Ren, J, Zou, Z, Yang, S, Ouyang, J, Zeng, W, Yang, B, Xiao, S and Huang, L 2009. Distribution of Escherichia coli F4 adhesion phenotypes in pigs of 15 Chinese and western breeds and a White Duroc×Erhualian intercross. Journal of Medical Microbiology 58, 1112–1117.Google Scholar