Hostname: page-component-848d4c4894-4rdrl Total loading time: 0 Render date: 2024-07-02T10:20:52.211Z Has data issue: false hasContentIssue false
  • English
  • Français

DNA barcoding of Podonomus (Chironomidae, Podonominae) enables stage association of a named species and reveals hidden diversity in Brazilian inselbergs

Published online by Cambridge University Press:  29 January 2013

Susana Trivinho-Strixino ,
Mateus Pepinelli ,
Tadeu Siqueira  and
Fabio de Oliveira Roque
Susana Trivinho-Strixino
Affiliation:
Laboratório de Ecologia de Insetos Aquáticos (LEIA), Departamento de Hidrobiologia, Universidade Federal de São Carlos, São Carlos, Cx. Postal: 676, CEP 13565-905, SP, Brazil
Mateus Pepinelli
Affiliation:
Laboratório de Ecologia de Insetos Aquáticos (LEIA), Departamento de Hidrobiologia, Universidade Federal de São Carlos, São Carlos, Cx. Postal: 676, CEP 13565-905, SP, Brazil
Tadeu Siqueira
Affiliation:
Departamento de Ecologia, Universidade Estadual Paulista, Avenida 24-A,1515, CEP13506-900, Rio Claro, SP, Brazil
Fabio de Oliveira Roque
Affiliation:
Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, CCBS, Cidade Universitária, Caixa Postal 549, CEP 79070-900, Campo Grande, MS, Brazil
Get access

Abstract

Podonomus Philippi was recently found in Brazilian inselbergs. In this study, we used sequences from the barcode region of cytochrome c oxidase I (COI) to assess Podonomus molecular diversity from mountains in Southeastern Brazil. Our results revealed the adult female and the larvae of Podonomus pepinellii Roque and Trivinho-Strixino, 2004 and extended the known geographical distribution of the species. Moreover, we found high molecular divergence in two populations located in the Serra da Mantiqueira and Serra do Espinhaço mountain range when compared with the only species recorded to Brazil, indicating the possibility of two more species to be described.

Keywords

Dipteramolecular identificationimmature stagescytochrome c oxidase I
Type
Research Article
Information
Annales de Limnologie - International Journal of Limnology , Volume 48 , Issue 4 , 2012 , pp. 411 - 423
Copyright
© EDP Sciences, 2013

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ashe, P. and O'Connor, J.P., 2009. A world catalogue of Chironomidae (Diptera). Part 1. Buchonomyiinae, Chilenomyiinae, Podonominae, Aphroteniinae, Tanypodinae, Usambaromyiinae, Diamesinae, Prodiamesinae and Telmatogetoninae. Irish Biogeographical Society and National Museum of Ireland, Dublin, 445 p.Google Scholar
Brundin, L., 1966. Transantarctic relationships and their significance, as evidenced by chironomid midges with a monograph of the subfamilies Podonominae and Aphroteniinae and the austral Heptagyiae. Kunglica Svenska Vetenskapsakademiens Handlingar, 11, 1–472.Google Scholar
Cranston, P.S., Hardy, N.B., Morse, G.E., Puslednik, L. and McCluen, S.R., 2010. When morphology and molecules concur: the ‘Gondwanan’ midges. (Diptera: Chironomidae). Syst. Entomol., 35, 636–648.CrossRefGoogle Scholar
Ekrem, T., Willassen, E. and Stur, E., 2007. A comprehensive DNA sequence library is essential for identification with DNA barcodes. Mol. Phylogenet. Evol., 43, 530–542.CrossRefGoogle ScholarPubMed
Ekrem, T., Stur, E. and Hebert, P.D.N., 2010. Females do count: documenting Chironomidae (Diptera) species diversity using DNA barcoding. Org. Divers. Evol., 10, 397–408.CrossRefGoogle Scholar
Hamada, N., Pepinelli, M., Mattos-Glória, A. and Luz, S.L.B., 2010. A new black fly species from Brazil, closely related to Simulium guianense Wise (Diptera, Simuliidae), revealed by morphology and DNA barcoding. Zootaxa, 2428, 22–36.Google Scholar
Hebert, P.D.N., Penton, E.H., Burns, J.M., Janzen, D.H. and Hallwachs, W., 2004. Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proc. Natl. Acad. Sci. USA, 101, 14812–14817.CrossRefGoogle ScholarPubMed
Kimura, M., 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide-sequences. J. Mol. Evol., 16, 111–120.CrossRefGoogle ScholarPubMed
Laurindo, F.S., Wiedenbrug, S., Trivinho-Strixino, S., Neubern, C.S.O. and Pepinelli, M., 2012. Two new species of Hudsonimyia Roback, 1979 (Diptera: Chironomidae: Tanypodinae) from neotropical region unveiled by DNA barcoding. J. Nat. Hist. (to be published on May 2012)Google Scholar
Martinelli, G., 2007. Mountain biodiversity in Brazil. Rev. Bras. Bot., 30, 587–597.CrossRefGoogle Scholar
Roque, F.O. and Trivinho-Strixino, S., 2004. Podonomus pepinellii n. sp., first record of the genus and subfamily from Brazil (Diptera: Chironomidae: Podonominae). Zootaxa, 689, 1–7.Google Scholar
Sæther, O.A., 1980. Glossary of chironomid morphology terminology (Diptera: Chironomidae). Ent. Scand., Suppl. 14, 1–51.Google Scholar
Siri, A., Paggi, A. and Donato, M., 2009. Podonomus fastigians (Chironomidae: Podonominae): redescription of the adult male and female, the pupa, and description of the larva. Entomol. News, 120, 522–529.CrossRefGoogle Scholar
Stur, E. and Ekrem, T., 2011. Exploring unknown life stages of Arctic Tanytarsini (Diptera: Chironomidae) with DNA barcoding. Zootaxa, 2743, 27–39.Google Scholar
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S., 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol., 28, 2731–2739.CrossRefGoogle ScholarPubMed
Wiedenbrug, S., Mendes, H.F., Pepinelli, M. and Trivinho-Strixino, S., 2009. Review of the genus Onconeura Andersen et Sæther (Diptera: Chironomidae), with the description of four new species from Brazil. Zootaxa 2265, 1–26.Google Scholar