A small freshwater pond near Palmer Station, Anvers Island, (64° 45'S, 64° 05'W) was sampled every 6 h over a 24 h period during the late austral summer to examine changes in both neuston and plankton population levels and activity. Total bacteria using acridine orange and epifluorescence microscopy, counts of viable colony-forming units, and chlorophyll a were determined. Activity was estimated by measuring the rate of 3H-thymidine incorporation. The data indicate that over this time period the bacterioneuston populations went through a diel fluctuation and temperature may be an important factor in this cycle. Although the bacterioneuston populations were often more numerous than corresponding plankton populations, subsurface bacteria appeared to be metabolically more active.