In captive Heterobranchus 1ongifilis females, oocytes maturation and ovulation are induced by a single injection of human chorionic gonadotropin (hCG) at a dose of 1.5 I.U./g body weight. In the present study, three experiments were performed in order to specify the timing of ovulation and the effects of varying latency period on the quantity and quality of the collected ova. The ova quality was estimated by hatching percentage and proportion of deformed larvae obtained after artificial fertilization. The results showed that, in an individual female, ovulation of postvitellogenic oocytes is not synchronous and takes 3 to 4 hours to be completed, between 7–8 h and 11 h after hCG injection at 30 °C. The quantity of ova that could be collected by handstripping increased with the latency period, from 18,000 ± 12,000 to 72,000 ± 29,000 ova per female at 8 h and 11 h after injection, respectively. However, the first ova obtained (8 h after injection) were of good quality (94% hatching) and showed no signs of incomplete final maturation. Eleven hours after injection, the percentages of ovulated oocytes within the whole ovarian population of postvitellogenic oocytes were high in all the females, being generally around 90%. After ovulation, aging of ova occurred rapidly: the proportion of deformed larvae increased significantly (from 4 to 20%) 2 h after completion of ovulation and, 4 h post-ovulation, hatching percentage dropped from 92 to 36%. In H. longifilis, the optimal latency period can be defined as the time, from injection to stripping, allowing the best compromise between quantity and quality of ova released. This latency period was found to be 11 h following hCG injection at 30 °C, and corresponds approximately to the completion of ovulation.