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Link between exposure of fish (Solea solea) to PAHs and metabolites: Application to the “Erika” oil spill

Published online by Cambridge University Press:  15 October 2004

Hélène Budzinski ,
Olivier Mazéas ,
Jacek Tronczynski ,
Yves Désaunay ,
Gilles Bocquené  and
Guy Claireaux
Hélène Budzinski
Affiliation:
Laboratoire de Physico- et Toxico-Chimie des Systèmes Naturels, UMR 5472 CNRS, Université Bordeaux 1, 351 cours de la Libération, 33405 Talence, France
Olivier Mazéas
Affiliation:
Laboratoire de Physico- et Toxico-Chimie des Systèmes Naturels, UMR 5472 CNRS, Université Bordeaux 1, 351 cours de la Libération, 33405 Talence, France IFREMER, DEL/PC, Centre de Nantes, rue de l'Ile d'Yeu, BP 21105, 44311 Nantes Cedex 3, France
Jacek Tronczynski
Affiliation:
IFREMER, DEL/PC, Centre de Nantes, rue de l'Ile d'Yeu, BP 21105, 44311 Nantes Cedex 3, France
Yves Désaunay
Affiliation:
IFREMER, Laboratoire d'Ecologie Halieutique, Centre de Nantes, rue de l'Ile d'Yeu, BP 21105, 44311 Nantes Cedex 3, France
Gilles Bocquené
Affiliation:
IFREMER, DEL/PC, Centre de Nantes, rue de l'Ile d'Yeu, BP 21105, 44311 Nantes Cedex 3, France
Guy Claireaux
Affiliation:
CREMA, Place du Séminaire, BP 5, 17137 L'Houmeau, France
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Abstract

An analytical method consisting in enzymatic deconjugation, solid phase extraction and purification, and gas chromatography/mass spectrometry analysis after derivatization was used in this study to quantify Polycyclic Aromatic Hydrocarbon (PAH) metabolites in the bile of fish. The method has been applied in a laboratory experiment studying the fate of pyrene in basin containing soles. This study has allowed the identification of 1-hydroxypyrene as the single metabolite in bile after enzymatic deconjugation. In a second time, 1-hydroxypyrene has been used as a biomarker of exposure in the case of the “Erika” oil spill. This biomonitoring was successful in demonstrating the exposure of juvenile soles to PAHs present in the “Erika” fuel oil.

Keywords

PAH metabolitesFishGas chromatography-mass spectrometry
Type
Research Article
Information
Aquatic Living Resources , Volume 17 , Issue 3: The "Erika" Oil Spill: Environmental Contamination and Effects in the Bay of Biscay , July 2004 , pp. 329 - 334
Copyright
© EDP Sciences, IFREMER, IRD, 2004

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