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The analysis of α-tocopherol and ubiquinone in rat liver

Published online by Cambridge University Press:  09 March 2007

A. T. Diplock ,
J. Green ,
J. Bunyan  and
D. McHale
A. T. Diplock
Affiliation:
Walton Oaks Experimental Station, Vitamins Limited, Dorking Road, Tadworth, Surrey
J. Green
Affiliation:
Walton Oaks Experimental Station, Vitamins Limited, Dorking Road, Tadworth, Surrey
J. Bunyan
Affiliation:
Walton Oaks Experimental Station, Vitamins Limited, Dorking Road, Tadworth, Surrey
D. McHale
Affiliation:
Walton Oaks Experimental Station, Vitamins Limited, Dorking Road, Tadworth, Surrey
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Abstract

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1. A comparative study has been made of five methods of saponifying normal and vitamin E-deficient rat liver and subsequently measuring ubiquinone and α-tocopherol. 2. Losses of ubiquinone and α-tocopherol occur under certain conditions, and three of the methods were judged to be unsatisfactory. Recoveries of α-tocopherol are nearly quantitative under the conditions of ToŠić & Moore (1945), but methods that use large reaction times or weaker alkali destroy tocopherol, and over 50 % loss can be encountered. Ubiquinone does not seem as sensitive to the time of reaction, but is sensitive to the concentration of alkali and amount of water present during saponification.

Type
Research Article
Information
British Journal of Nutrition , Volume 20 , Issue 1 , February 1966 , pp. 95 - 101
Copyright
Copyright © The Nutrition Society 1966

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