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Brain glutamate and γ-aminobutyrate (GABA) metabolism in thiamin-deficient rats

Published online by Cambridge University Press:  09 March 2007

Martyn G. Page ,
Victor Ankoma-Sey ,
William F. Coulson  and
David A. Benders
Martyn G. Page
Affiliation:
Department of Biochemistry, University College and Middlesex School of Medicine, University College London, Gower Street, London WClE 6BT,
Victor Ankoma-Sey
Affiliation:
Department of Biochemistry, University College and Middlesex School of Medicine, University College London, Gower Street, London WClE 6BT,
William F. Coulson
Affiliation:
Department of Biochemistry, University College and Middlesex School of Medicine, University College London, Gower Street, London WClE 6BT,
David A. Benders
Affiliation:
Department of Biochemistry, University College and Middlesex School of Medicine, University College London, Gower Street, London WClE 6BT,
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Abstract

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The brain metabolism of glutamate and γ-aminobutyrate (GABA) was investigated in thiamin-deficient and pair-fed control rats, in order to determine whether the GABA shunt may provide an important alternative to 2-oxo-glutarate dehydrogenase (EC 1.2.4.2) in energy-yielding metabolism in thiamin deficiency. Brains from thiamin-deficient animals contained less glutamate, 2-oxo-glutarate and GABA than those from control animals. The brain content of ATP was unaffected by thiamin deficiency. After intracerebroventricular injection of [14C]glutamate, the specific radioactivity of GABA in the brains from deficient animals was 45–50% higher than that in controls, suggesting a considerable increase in the metabolic flux through the GABA shunt in thiamin deficiency. Brain GABA showed a marked circumannual variation, with a peak in mid-summer and a minimum value in mid-winter.

Keywords

y-AminobutyrateBrain glutamateGABAThiamin deficiencyRat
Type
Amino Acids and Proteins: Metabolism and Requirements
Information
British Journal of Nutrition , Volume 62 , Issue 2 , September 1989 , pp. 245 - 253
Copyright
Copyright © The Nutrition Society 1989

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