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Changes in the urinary excretion of acid-soluble peptides in rats injected with streptozotocin or dexamethasone: a trial to estimate the changes in the rate of whole-body protein degradation in those rats

Published online by Cambridge University Press:  09 March 2007

Taek Jeong Nam ,
Tadashi Noguchi  and
Hiroshi Naito
Taek Jeong Nam
Affiliation:
Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan
Tadashi Noguchi
Affiliation:
Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan
Hiroshi Naito
Affiliation:
Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan
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Abstract

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Urinary excretion of acid-soluble peptides (ASP) was measured in rats given streptozotocin or dexamethasone. Streptozotocin-induced diabetic rats excreted increased amounts of urinary nitrogen and ASP-form amino acids. The urinary ratio for N: ASP-form leucine plus valine, which has been shown to reflect the efficiency of dietary N utilization, increased in the diabetic rats, suggesting the impaired utilization of dietary N (and re-utilization of endogenous N). Dexamethasone administration to adrenalectomized rats caused increased excretion of urinary ASP-form leucine plus valine with a concomitant increase in N excretion. However, urinary ratio for N: ASP-form leucine plus valine did not change significantly. The results suggested that dexamethasone caused increased degradation of body proteins, but maintained the efficiency of dietary N utilization or re-utilization of endogenous N. Based on the present observations and the hypothesis proposed previously by the present authors (Noguchi et al. 1988) that urinary excretion of ASP-form leucine plus valine reflects the rate of whole-body protein degradation and the urinary ratio for N: ASP-form leucine plus valine represents N utilization, the origin of urinary N in streptozotocin-diabetic and dexamethasone-administered rats is discussed

Keywords

Urinary acid-soluble peptidesDiabetesDexamethasoneProtein degradationRat
Type
Protein Metabolism
Information
British Journal of Nutrition , Volume 65 , Issue 1 , January 1991 , pp. 37 - 46
Copyright
Copyright © The Nutrition Society 1991

References

REFERENCES

Flaim, K. E., Copenhaver, M. E. & Jefferson, L. S. (1980). Effects of diabetes on protein synthesis in fast- and slow-twitch rat skeletal muscle. American Journal of Physiology 239, E88–E95.Google ScholarPubMed
Garlick, P. J., Preedy, V. R. & Reeds, P. J. (1985). Regulation of protein turnover in vivo by insulin and amino acids. In Intracellular Protein Catabolism, pp. 555 564 [Khairallah, E. A.,Bond, J. S. and Bird, J. W. C., editors]. New York: Alan R. Liss.Google Scholar
Manchester, K. L. (1970). Site of hormonal regulation of protein metabolism. In Mammalian Protein Metabolism, vol. 4, pp. 254–262 [Munro, H. N., editor]. New York and London: Academic Press.Google Scholar
Millward, D. J., Garlick, P. J., Nnanyelugo, D. O. & Waterlow, J. C. (1976). The relative importance of muscle protein synthesis and breakdown in the regulation of muscle mass. Biochemical Journal 156, 185–188.CrossRefGoogle ScholarPubMed
Millward, D. J. & Waterlow, J. C. (1978). Effect of nutrition on protein turnover in skeletal muscle. Federation Proceedings 37, 2283–2290.Google ScholarPubMed
Munro, H. N. (1964). General aspects of the regulation of protein metabolism by diet and by hormones. In Mammalian Protein Metabolism, vol. 1, pp. 381–481 [Munro, H. N. and Allison, J. B., editors]. New York and London: Academic Press.CrossRefGoogle Scholar
Nagasawa, T., Kadowaki, M., Noguchi, T. & Naito, H. (1982). The effect of insulin on myofibrillar protein degradation in normal and streptozotocin-induced diabetic rats measured by the rate of NT-methylhistidine release from perfused hindquarters. Agricultural and Biological Chemistry 46, 3023–3027.Google Scholar
Nishizawa, N., Shimbo, M., Noguchi, T., Hareyama, S. & Funabiki, R. (1978). Effect of starvation, refeeding and hydrocortisone administration on turnover of myofibrillar proteins estimated by urinary excretion of NT-methylhistidine in the rat. Agricultural and Biological Chemistry 42, 2083–2089.Google Scholar
Noguchi, T., Nam, T. J., Kato, H. & Naito, H. (1988). Further studies on the nutritional factors affecting the urinary excretion of acid-soluble peptides in rats. British Journal of Nutrition 60, 321–337.CrossRefGoogle ScholarPubMed
Noguchi, T., Okiyama, A., Naito, H., Kaneko, K. & Koike, G. (1982). Some nutritional and physiological factors affecting the urinary excretion of acid-soluble peptides in rat and women. Agricultural and Biological Chemistry 46, 2821–2828.Google Scholar
Odedra, B. R., Bates, P. C. & Millward, D. J. (1983). Time course of the effect of catabolic doses of corticosterone on protein turnover in rat skeletal muscle and liver. Biochemical Journal 214, 617–627.CrossRefGoogle ScholarPubMed
Odedra, B. R. & Millward, D. J. (1982). Effect of corticosterone treatment on muscle protein turnover in adrenalectomized rats and diabetic rats maintained on insulin. Biochemical Journal 204, 663–672.CrossRefGoogle ScholarPubMed
Pain, V. M., Albertse, E. C. & Garlick, P. J. (1983). Protein metabolism in skeletal muscle, diaphragm, and heart of diabetic rats. American Journal of Physiology 245, E604–E610.Google ScholarPubMed
Pain, V. M. & Garlick, P. J. (1974). Effect of streptozotocin diabetes and insulin treatment on the rate of protein synthesis in tissues of the rat in vivo. Journal of Biological Chemistry 249, 4501–4514.CrossRefGoogle ScholarPubMed
Rogers, Q. R. & Harper, A. E. (1965). Amino acid diets and maximal growth in the rat. Journal of Nutrition 87, 267–273.CrossRefGoogle ScholarPubMed
Snedecor, G. W. & Cochran, W. G. (1967 a). Factorial experiments. The split-plot or nested design. In Statistical Methods, 6th ed., pp. 369–375. Ames, Iowa: Iowa State University Press.Google Scholar
Snedecor, G. W. & Cochran, W. G. (1967 b). Factorial experiments. The general two-factor experiments. In Statistical Methods, 6th ed., pp. 346–349. Ames, Iowa: Iowa State University Press.Google Scholar
Snedecor, G. W. & Cochran, W. G. (1967 c). One-way classifications. Analysis of variance. Inspection of all differences between pairs of means. In Statistical Methods, 6th ed., pp. 271–273. Ames, Iowa: Iowa State University Press.Google Scholar
Tomas, F. M., Munro, H. N. & Young, V. R. (1979). Effect of glucocorticoid administration on the rate of muscle protein breakdown in vivo in rats, as measured by urinary excretion of NT-methylhistidine. Biochemical Journal 178, 139–146.CrossRefGoogle Scholar
Tomas, F. M., Murray, A. J. & Jones, L. M. (1984). Modification in glucocorticoid-induced changes in myofibrillar protein turnover in rats by protein and energy deficiency as assessed by urinary excretion of NTmethylhistidine. British Journal of Nutrition 51, 323–337.CrossRefGoogle Scholar
Waterlow, J. C., Garlick, P. J. & Millward, D. J. (1978). The effects of nutrition and hormones on protein turnover in muscle. In Protein Turnover in Mammalian Tissues and in the Whole Body, pp. 667–681. Amsterdam, New York and Oxford: North-Holland.Google Scholar