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Differences in labelled triolein turnover after oral administration between liver and adipose tissue of rats

Published online by Cambridge University Press:  08 March 2007

Nobuko Iritani ,
Tomoe Kimura ,
Hitomi Fukuda  and
Tomomi Sugimoto
Nobuko Iritani*
Affiliation:
Faculty of Human and Cultural Studies, Tezukayama Gakuin University, 4-Cho, Harumidai, Sakai, Osaka 590-0113, Japan
Tomoe Kimura
Affiliation:
Faculty of Human and Cultural Studies, Tezukayama Gakuin University, 4-Cho, Harumidai, Sakai, Osaka 590-0113, Japan
Hitomi Fukuda
Affiliation:
Faculty of Human and Cultural Studies, Tezukayama Gakuin University, 4-Cho, Harumidai, Sakai, Osaka 590-0113, Japan
Tomomi Sugimoto
Affiliation:
Faculty of Human and Cultural Studies, Tezukayama Gakuin University, 4-Cho, Harumidai, Sakai, Osaka 590-0113, Japan
*
*Corresponding author: Dr Nobuko Iritani, fax +81 72 292 2135, email iritani@hcs.tezuka-gu.ac.jp
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Abstract

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To investigate exogenous triacylglycerol turnover, the time courses for labelled triolein in the liver, plasma and epididymal adipose tissue (adipose tissue) after oral administration to rats fed a fat-free or 10 % corn oil diet for 3 d after fasting overnight were examined for 10 d. After the administration of labelled triolein to rats fed the fat-free diet, the incorporation (dpm/g) into total lipids of the liver and adipose tissue each reached the maximum in 8 h and was seven times higher in the adipose tissue than in the liver. The half-lives of total lipid radioactivities during the decreasing phases were 0·39 and 2·58 d, respectively, in the rapid and slow phases of the decay curve in the liver, and 4·78 d in only one phase of the adipose tissue. Radioactivity after administration of labelled triolein was mostly found in the oleic acid in the tissues. The half-life of oleic acid was 3·92 d in the adipose tissues. These half-lives were similar in both dietary groups. Thus, although dietary corn oil reduced the triolein incorporation to cellular lipids in comparison to the fat-free diet, it did not affect these half-lives. The labelled triacylglycerol–oleic acid stayed abundantly intact for a long time in the adipose tissue and was scarcely changed to other fatty acids, whereas it was slightly incorporated into total lipids and quickly metabolized in the liver. Non-essential fatty acids may be mostly endogenous in the liver but may be exogenous and endogenous in adipose tissue.

Keywords

Labelled trioleinHalf-lifeLiverAdipose tissue
Type
Research Article
Information
British Journal of Nutrition , Volume 93 , Issue 1 , January 2005 , pp. 53 - 58
Copyright
Copyright © The Nutrition Society 2005

References

Baker, N, Learn, DB & Bruckdorfer, KR (1978) Re-evaluation of lipogenesis from dietary glucose carbon in liver and carcass of mice. J Lipid Res 19, 879–893.CrossRefGoogle ScholarPubMed
Baker, N, Mead, J, Kannan, B (1981) Hepatic contribution to newly made fatty acids in adipose tissue in rats and inhibition of hepatic and extrahepatic lipogenesis from glucose by dietary corn oil. Lipids 16, 568–576.Google Scholar
Borensztain, J & Getz, GS (1972) The contribution of lipogenesis in situ to the accumulation of fat by rat adipose tissue. Biochim Biophys Acta 280, 86–93.CrossRefGoogle Scholar
Carmaniu, S & Herrera, E (1980) Comparative utilization in vivo of [U- 14 C]glycerol, [2- 3 H]glycerol, [U- 14 C]glucose and [1- 14 C]palmitate in the rat. Arch Int Physiol Biochim 88, 255–263.Google Scholar
Folch, J, Lees, M, Sloane-Stanley, GH (1957) A simple method for isolation and purification of total lipids from animal tissues. J Biol Chem 226, 497–509.Google Scholar
Gordis, E (1965) The long-term stability of triglyceride molecules in adipose tissue. J Clin Invest 44, 1978–1985.CrossRefGoogle ScholarPubMed
Hems, DA, Rath, EA & Verrinder, TR (1975) Fatty acid synthesis in liver and adipose tissue of normal and genetically obese (ob/ob) mice during the 24-hour cycle. Biochem J 150, 167–173.CrossRefGoogle ScholarPubMed
Iritani, N (1993) A review: Nutritional and hormonal regulation of lipogenic enzyme gene expression in rat liver. Eu J Biochem 205, 433–442.Google Scholar
Iritani, N, Fukuda, H & Tada, K (1996) Nutritional regulation of lipogenic enzyme gene expression in epididymal adipose tissue. J Biochem (Tokyo) 120, 242–248.CrossRefGoogle ScholarPubMed
Iritani, N, Kimura, T, Fukuda, H & Sugimoto, T (2000) Effects of dietary fatty acid on tissue fatty acid compositions in rats. J Jpn Soc Food Sci 53, 249–257.Google Scholar
Iritani, N, Komiya, M, Fukuda, H & Sugimoto, T (1998) Lipogenic enzyme gene expression is quickly suppressed in rats by a small amount of exogenous polyunsaturated fatty acids. J Nutr 128, 967–972.Google Scholar
Kalopissis, AD, Grioglio, S, Malewiak, MI, Rozen, R & Liepvre, XL (1981) Very-low-density-lipoprotein secretion by isolated hepatocytes of fat-fed rats. Biochem J 198, 373–377.CrossRefGoogle ScholarPubMed
Kannan, R, Elovson, J, Learn, DB & Baker, N (1980) Fatty acid synthesis in vivo and hepatic contribution to whole-body lipogenic rates in obese Zucker rats. Lipids 15, 993–998.Google Scholar
Lambert, MS, Avella, MA, Botham, KM & Mayes, PA (1998) Comparison of short- and long-term effects of different dietary fats on the hepatic uptake and metabolism of chylomicron remnants in rats. Br J Nutr 79, 203–211.Google Scholar
National Research Council (1985) Guide for the Care and Use of Laboratory Animals Publication no. 85–23 (rev.) Bethesda, MD National Institute of Health.Google Scholar
Pedersen, NT & Marqversen, J (1981) Metabolism of ingested 14 C-triolein. Estimation of radiation dose in tests of lipid assimilation using 14 C- and 3 H-labeled fatty acids. Eur J Nucl Med 6, 327–329.Google Scholar
Reeves, PG, Nielsen, FH, Fahey GC, Jr (1993) Purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformation of the AIN-76A rodent diet. J Nutr 123, 1939–1951.Google Scholar
Snedecor, GW & Cochran, WG (1967) Statistical Methods, pp. 285–338Ames: Iowa State University Press.Google Scholar