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The effect of a high dose of 3-hydroxy-3-methylbutyrate on protein metabolism in growing lambs

Published online by Cambridge University Press:  09 March 2007

Isabelle Papet ,
Piotr Ostaszewski ,
Francoise Glomot ,
Christiane Obled ,
Magali Faure ,
Gerard Bayle ,
Steven Nissen ,
Maurice Arnal  and
Jean Grizard
Isabelle Papet
Affiliation:
Laboratoire d'Etude du Métabolisme Azoté, Institut National de la Recherche Agronomique, Centre de Recherches de Clermont-Ferrand Theix, 63122 Saint-Genès-Champanelle, France
Piotr Ostaszewski
Affiliation:
Department of Animal Physiology, Warsaw Agricultural University, 02-766 Warsaw, Poland
Francoise Glomot
Affiliation:
Laboratoire d'Etude du Métabolisme Azoté, Institut National de la Recherche Agronomique, Centre de Recherches de Clermont-Ferrand Theix, 63122 Saint-Genès-Champanelle, France
Christiane Obled
Affiliation:
Laboratoire d'Etude du Métabolisme Azoté, Institut National de la Recherche Agronomique, Centre de Recherches de Clermont-Ferrand Theix, 63122 Saint-Genès-Champanelle, France
Magali Faure
Affiliation:
Laboratoire d'Etude du Métabolisme Azoté, Institut National de la Recherche Agronomique, Centre de Recherches de Clermont-Ferrand Theix, 63122 Saint-Genès-Champanelle, France
Gerard Bayle
Affiliation:
Laboratoire d'Etude du Métabolisme Azoté, Institut National de la Recherche Agronomique, Centre de Recherches de Clermont-Ferrand Theix, 63122 Saint-Genès-Champanelle, France
Steven Nissen
Affiliation:
Department of Animal Science, Iowa State University, Ames, IA 50011, USA
Maurice Arnal
Affiliation:
Laboratoire d'Etude du Métabolisme Azoté, Institut National de la Recherche Agronomique, Centre de Recherches de Clermont-Ferrand Theix, 63122 Saint-Genès-Champanelle, France
Jean Grizard
Affiliation:
Laboratoire d'Etude du Métabolisme Azoté, Institut National de la Recherche Agronomique, Centre de Recherches de Clermont-Ferrand Theix, 63122 Saint-Genès-Champanelle, France
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Abstract

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The effect of a high dose of 3-hydroxy-3-methylbutyrate (HMB, a leucine catabolite) on protein metabolism was investigated in growing male lambs fed on hay and concentrate. Concentrate was supplemented with either Ca(HMB)2 (4g/kg) or Ca(C03)2 in experimental (HMB) and control groups respectively. Both groups consisted of six 2-month old lambs. Three complementary methods to study protein metabolism were carried out consecutively 2·5 months after beginning the dietary treatment: whole body phenylalanine fluxes, postprandial plasma free amino acid time course and fractional rates of protein synthesis in skeletal muscles. Feeding a high dose of HMB led to a significant increase in some plasma free amino acids compared with controls. Total, oxidative and non-oxidative phenylalanine fluxes were not modified by dietary HMB supplementation. Similarly, an acute infusion of HMB, in the control group, did not change these fluxes. In skeletal muscles, fractional rates of protein synthesis were not affected by long-term dietary supplementation with HMB. Taken together our results showed that administration of a high dose of HMB to lambs was able to modify plasma free amino acid pattern without any effect on whole-body protein turnover and skeletal muscle protein synthesis

Keywords

3-Hydroxy-3-methylbutyrateAmino acidsProtein turnoverProtein synthesis
Type
Animal Nutrition
Information
British Journal of Nutrition , Volume 77 , Issue 6 , June 1997 , pp. 885 - 896
Copyright
Copyright © The Nutrition Society 1997

References

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