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Effect of defaunation on the metabolism of rumen micro-organisms

Published online by Cambridge University Press:  09 March 2007

D. I. Demeyer  and
C. J. Van Nevel
D. I. Demeyer
Affiliation:
Laboratoriurn voor Voeding en Hygiene, Rijksuniversiteit Gent Proefhoevestraat, 10 B-9230 Melle, Belgium
C. J. Van Nevel
Affiliation:
Laboratoriurn voor Voeding en Hygiene, Rijksuniversiteit Gent Proefhoevestraat, 10 B-9230 Melle, Belgium
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Abstract

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1. Rumen contents of a fasted fistulated wether, obtained in a faunated, defaunated and refaunated period were incubated in vitro with a mixture of cellobiose and maltose, in the presence of ammonium bicarbonate and 32PO43−. Total synthesis of microbial N (Nt) was calculated from 32P incorporation and N:P determined in microbial matter. The N:P value was not affected by defaunation. Net synthesis of microbial N (Nn) was calculated from ammonia-N incorporation. An estimate of degradation of microbial N was calculated as Nt–Nn. Energetic efficiency of synthesis was calculated from the volatile fatty acids produced during incubation, as g N incorporated per kg organic matter fermented (g N/kg OMf).

2. Defaunation decreased the proportions of acetate, butyrate and methane and increased those of propionate in fermentation end-products. Fermentation rate when expressed per mg microbial N was not affected by defaunation.

3. Expressed per unit volume of rumen contents, Nn was increased by defaunation whereas Nf remained unchanged. Thus, a decrease in degradation can be calculated. Energetic efficiences of total and net synthesis were increased from 35 and 13 to 47 and 30 g N/kg OMf respectively.

4. Specific rates of both total and net synthesis of microbial N were significantly increased by defaunation whereas the specific rate of degradation was not affected.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 42 , Issue 3 , November 1979 , pp. 515 - 524
Copyright
Copyright © The Nutrition Society 1979

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