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The effects of iron deficiency and iron overload on cell-mediated immunity in the mouse

Published online by Cambridge University Press:  09 March 2007

Felix O. Omara  and
Barry R. Blakley
Felix O. Omara
Affiliation:
Department of Veterinary Physiological Sciences, Western College of Veterinary Medicine, University of Saskatchewan,Saskatoon S7N OW0, Canada
Barry R. Blakley
Affiliation:
Department of Veterinary Physiological Sciences, Western College of Veterinary Medicine, University of Saskatchewan,Saskatoon S7N OW0, Canada
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Abstract

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The influence of Fe status on cell-mediated immunity was studied in weanling mice fed on Fe-deficient (7 mg Fe/kg), Fe-sufficient (120 mg Fe/kg) and high-Fe (3000 or 5000 mg Fe/kg) diets for 7 weeks. The contact sensitivity (CS) response to dinitrofluorobenzene (DNFB), the in vivo delayed-type hypersensitivity (DTH) response to sheep erythrocytes (SRBC) and the ability of primed spleen cells to transfer DTH response to naive normal mice were suppressed in mice consuming the Fe-deficient diet. High-Fe diets (3000 or 5000 mg Fe/kg) selectively suppressed the CS response to DNFB, but the DTH response to SRBC or the transfer of DTH response by primed spleen cells to naive normal mice remained normal. Spleen cell functions associated with the expression of class II major histocompatibility (MHC) surface antigens, concanavalin A-induced interleukin-2 (IL-2) secretion or the antigen-presenting cell (APC) ability to stimulate antigen-dependent proliferation of an SRBC-specific helper T-lymphocyte clone were not altered by Fe status. However, consistent with the suppressed DTH response in the Fe- deficient mice was the suppressed concanavalin A-induced T-lymphocyte blastogenesis and the interferon-γ (INF-γ) production by spleen cells from mice fed on the Fe-deficient diet. Spleen cells from mice fed on excess levels of Fe in the diet secreted less INF-γ than the control mice, although T- lymphocyte proliferation remained unaffected. Suppression of the cellular immune response associated with Fe deficiency may be related in part to impaired T-lymphocyte proliferation and INF-γ secretion rather than to deficits in IL-2 secretion or APC function.

Keywords

Immune functionIron deficiencyIron overload
Type
Iron Status and cell-mediated immunity
Information
British Journal of Nutrition , Volume 72 , Issue 6 , December 1994 , pp. 899 - 909
Copyright
Copyright © The Nutrition Society 1994

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