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Elements of Mediterranean diet improve oxidative status in blood of kidney graft recipients

Published online by Cambridge University Press:  08 March 2007

Ewa Stachowska ,
Teresa Wesołowska ,
Maria Olszewska ,
Krzysztof Safranow ,
Barbara Millo ,
Leszek Domański ,
Katarzyna Jakubowska ,
Kazimierz Ciechanowski  and
Dariusz Chlubek
Ewa Stachowska*
Affiliation:
Department of Biochemistry and Chemistry, Pomeranian Medical University, Szczecin, Poland
Teresa Wesołowska
Affiliation:
Department of Clinical Biochemistry and Laboratory Diagnostics, Pomeranian Medical University, Szczecin, Poland
Maria Olszewska
Affiliation:
Department of Biochemistry and Chemistry, Pomeranian Medical University, Szczecin, Poland
Krzysztof Safranow
Affiliation:
Department of Biochemistry and Chemistry, Pomeranian Medical University, Szczecin, Poland
Barbara Millo
Affiliation:
Department of Clinical Biochemistry and Laboratory Diagnostics, Pomeranian Medical University, Szczecin, Poland
Leszek Domański
Affiliation:
Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczecin, Poland
Katarzyna Jakubowska
Affiliation:
Department of Biochemistry and Chemistry, Pomeranian Medical University, Szczecin, Poland
Kazimierz Ciechanowski
Affiliation:
Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczecin, Poland
Dariusz Chlubek
Affiliation:
Department of Biochemistry and Chemistry, Pomeranian Medical University, Szczecin, Poland
*
*Corresponding author: Dr Ewa Stachowska, fax +48 91 466 1516, email ewast@sci.pam.szczecin.pl
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Abstract

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Patients were fully informed as to the study objectives and benefits, and provided written consent prior to enrolment. The study protocol was approved by the Committee on Human Research at the Pomeranian Medical University, Szczecin, Poland. An intensification of free-radical reactions may contribute to accelerated atherosclerosis in kidney graft recipients. We examined the effect of a Mediterranean-type diet (MD) on the oxidative status of the plasma and erythrocytes of kidney graft recipients. Two patient groups were formed: a study group consuming the MD diet and a control group with a low-fat diet. C-reactive protein levels in plasma, oleic acid C18: 1n-9 and linoleic acid C18: 2n-6 concentrations in triacyloglycerols were determined. To determine the oxidative status, we measured the concentrations of α-tocopherol in plasma, the content of thiobarbituric acid-reactive species (TBARS) in plasma and erythrocytes, and the activities of superoxide dismutase, catalase and glutathione peroxidase in erythrocytes. In the MD group, the activities of erythrocyte enzymes changed significantly: those of superoxide dismutase increased (P<0.001 after 6 months), catalase decreased (P<0.001 after 6 months) and glutathione peroxidase decreased (P<0.05 after 2 months). The oleic acid content of triacylglycerols was increased (P<0.006) whereas that of linoleic acid was decreased (P<0.00005), α-tocopherol levels remaining unchanged. TBARS in plasma were decreased after 6 months of MD (P<0.05). No significant correlations were observed between TBARS, oleic acid, linoleic acid and α-tocopherol levels in plasma. MD appears to protect the erythrocytes against the action of free radicals, as reflected in the modified activities of some enzymes regulating the oxidative status of these blood cells.

Keywords

Mediterranean dietKidneyTransplantationSuperoxide dismutaseCatalaseGlutathione peroxidaseOleic acidLinoleic acidC-reactive proteinα-Tocopherol
Type
Research Article
Information
British Journal of Nutrition , Volume 93 , Issue 3 , March 2005 , pp. 345 - 352
Copyright
Copyright © The Nutrition Society 2005

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