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Excretion of purine derivatives by ruminants: effect of exogenous nucleic acid supply on purine derivative excretion by sheep

Published online by Cambridge University Press:  09 March 2007

X. B. Chen ,
F. D. DeB. Hovell ,
E. R. Ørskov  and
D. S. Brown
X. B. Chen
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
F. D. DeB. Hovell
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
E. R. Ørskov
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
D. S. Brown
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
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Abstract

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The present study examined the relationship between the supply of exogenous nucleic acid (NA) purines and their recovery as the derivatives hypoxanthine, xanthine, uric acid and allantoin in urine. Six lambs, totally nourished by intragastric infusions of volatile fatty acids (VFA) and casein (i.e. no rumen fermentation), were given by abomasal infusion a microbial NA concentrate at six levels (from zero to 24·5 mmol purines/d). The true digestibility between the abomasum and terminal ileum of the NA purines was measured in a separate experiment using three lambs. The relative proportion of urinary allantoin increased, and that of other derivatives decreased, as the amount of NA infused was increased. The relationship between total excretion of purine derivatives (Y; mmol/d) and exogenous purines absorbed (X; mmol/d) was Y = 0·84 X + 0.150W0·75e-0.25X, where W is body-weight (kg). This implies that the endogenous contribution to the total excretion of derivative decreased as the supply of exogenous purines increased, with an associated progressive replacement of de novo synthesis by exogenous purines. The model also implies that 0·16 of the purines were eliminated through routes other than derivative excretion in urine. Once excretion exceeded 0·6 mmol/kg W0·75 per d, endogenous excretion was effectively zero and thus Y = 0·84 X. In normally fed sheep, derivative excretion should therefore relate to the microbial purines and, hence, microbial protein absorbed according to these models. The changing proportions of allantoin and other derivatives in urine were probably due to changes in the relative importance of endogenous and exogenous purines as precursors.

Keywords

Nucleic acidsPurine derivativesSheep
Type
Metabolism in Ruminants
Information
British Journal of Nutrition , Volume 63 , Issue 1 , January 1990 , pp. 131 - 142
Copyright
Copyright © The Nutrition Society 1990

References

REFERENCES

Agricultural Research Council (1984). The Nutrient Requirements of Ruminant Livestock, Suppl. no. 1. Slough: Commonwealth Agricultural Bureaux.Google Scholar
Al-Khalidi, U.A.S. & Chaglassian, T.H. (1965). The species distribution of xanthine oxidase. Biochemical Journal 97, 318–320.CrossRefGoogle ScholarPubMed
Altman, P.L. & Dittmer, D.S. [editors] (1961). Blood and Other Body Fluids, pp. 400–414. Washington, DC: Federation of American Societies for Experimental Biology.Google Scholar
Antoniewicz, A.M., Heinemann, W.W. & Hanks, E.M. (1980). The effect of changes in the intestinal flow of nucleic acids on allantoin excretion in the urine of sheep. Journal of Agricultural Science, Cambridge 95, 395–400.CrossRefGoogle Scholar
Chen, X.B. (1989). Excretion of purine derivatives by sheep and cattle and its use for the estimation of absorbed microbial protein. PhD Thesis, University of Aberdeen.Google Scholar
Chen, X.B., Kyle, D.J., Whyte, C.C., Hovell, F.D. DeB. & Ørskov, E.R. (1989). Uric acid and allantoin in plasma and saliva of sheep. Proceedings of the Nutrition Society 48, 88A.Google Scholar
Chen, X.B., Ørskov, E.R. & Hovell, F.D. DeB. (1990). Excretion of purine derivatives by ruminants: endogenous excretion, differences between cattle and sheep. British Journal of Nutrition 63, 121–129.CrossRefGoogle ScholarPubMed
Condon, R.J., Hall, G. & Hatfield, E.E. (1970). Metabolism of abomasally infused 14C-labelled RNA adenine, uracil and glycine. Journal of Animal Science 31, 1037–1038 (abstr).Google Scholar
Fujihara, T., Ørskov, E.R. & Reeds, P.J. (1987). The effect of protein infusion on urinary excretion of purine derivatives in ruminants nourished by intragastric nutrition. Journal of Agricultural Science, Cambridge 109, 7–12.CrossRefGoogle Scholar
Giesecke, D., Stangassinger, M. & Tiemeyer, W. (1984). Nucleic acid digestion and urinary purine metabolites in sheep nourished by intragastric infusions. Canadian Journal of Animal Science 64, Suppl. 144–145.CrossRefGoogle Scholar
Hitchings, G.H. (1978). Uric acid: chemistry and synthesis. In Uric Acid, pp. 1–20 [Kelly, W.N. and Weiner, J.M., editors]. Berlin, Heidelberg and New York: Springer-Verlag.Google Scholar
Hovell, F.D. DeB., Ørskov, E.R., Kyle, D.J. & MacLeod, N.A. (1987). Undernutrition in sheep. Nitrogen repletion by N-depleted sheep. British Journal of Nutrition 57, 77–88.CrossRefGoogle ScholarPubMed
Lawes Agricultural Trust (1983). Genstat 4 User Manual. Rothamsted: Rothamsted Experimental Station.Google Scholar
Lindberg, J.E. (1985). Urinary allantoin excretion and digestible organic matter intake in dairy goats. Swedish Journal of Agricultural Research 15, 31–37.Google Scholar
McAllan, A.B. & Smith, R.H. (1973). Degradation of nucleic acid derivatives by rumen bacteria in vitro. British Journal of Nutrition 29, 467–474.CrossRefGoogle ScholarPubMed
Ørskov, E.R., Grubb, D.A., Wenham, G. & Corrigall, W. (1979). The sustenance of growing and fattening ruminants by intragastric infusion of volatile fatty acids and protein. British Journal of Nutrition 41, 553–558.CrossRefGoogle Scholar
Pentz, E.I. (1969). Adaptation of the Rimini-Schryver reaction for the measurement of allantoin in urine to the autoanalyzer: allantoin and taurine excretion following neutral irradiation. Analytical Biochemistry 27, 333–342.CrossRefGoogle Scholar
Razzaque, M.A., Topps, J.H., Kay, R.N.B. & Brockway, J.M. (1981). Metabolism of the nucleic acids of rumen bacteria by pre-ruminant and ruminant lambs. British Journal of Nutrition 45, 517–527.CrossRefGoogle Scholar
Ross, G.J.S. (1987). MLP User Manual. Rothamsted: Rothamsted Experimental Station.Google Scholar
Sibanda, S., Topps, J.H., Storm, E. & Ørskov, E.R. (1982). The excretion of allantoin by ruminants in relation to protein entering the abomasum. Proceedings of the Nutrition Society 41, 75A.Google Scholar
Smith, R.C., Moussa, N.M. & Hawkins, G.E. (1974). Utilization of the nucleic acids of Escherichia coli and rumen bacteria by sheep. Journal of Biological Chemistry 32, 529–537.Google ScholarPubMed
Stevenson, A.F. & deLangen, H. (1960). Measurement of feed intake by grazing cattle and sheep. VIII. Modified liver digestion method for determination of chromic oxide in faeces.. New Zealand Journal of Agricultural Research 3, 314–319.CrossRefGoogle Scholar
Storm, E., Brown, D.S. & Ørskov, E.R. (1983). The nutritive value of rumen micro-organisms in ruminants. 3. The digestion of microbial amino and nucleic acids in, and losses of endogenous nitrogen from, the small intestine of sheep. British Journal of Nutrition 50, 479–485.CrossRefGoogle ScholarPubMed
Storm, E. & Ørskov, E.R. (1983). The nutritive value of rumen microorganisms in ruminants. 1. Large-scale isolation and chemical composition of rumen micro-organisms. British Journal of Nutrition 50, 463–470.CrossRefGoogle ScholarPubMed
Technicon Instruments Co. (1979). Uric acid Technicon Method no. SD4-0013FM9. Tarrytown, NY: Technicon Instruments Co.Google Scholar
Uden, P., Colucci, P.E. & Van Soest, P.J. (1980). Investigation of chromium, cerium and cobalt as markers of digesta. Rate of passage studies. Journal of the Science of Food and Agriculture 31, 625–632.CrossRefGoogle ScholarPubMed
Wilson, D.W. & Wilson, H.C. (1962). Studies in vitro of the digestion and absorption of purine ribonucleotides by the intestine. Journal of Biological Chemistry 237, 1643–1647.CrossRefGoogle ScholarPubMed