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In vitro availability of zinc from infant foods with increasing phytic acid contents

Published online by Cambridge University Press:  09 March 2007

Donwina Bosscher ,
Zhengli Lu ,
Geert Janssens ,
Micheline Van Caillie-Bertrand ,
Harry Robberecht ,
Herman De Rycke ,
Roland De Wilde  and
Hendrik Deelstra
Donwina Bosscher*
Affiliation:
Department of Pharmaceutical Sciences, Laboratory of Food Sciences, University of Antwerp (UIA), Universiteitsplein 1, 2610 Antwerp (Wilrijk), Belgium
Zhengli Lu
Affiliation:
Department of Pharmaceutical Sciences, Laboratory of Food Sciences, University of Antwerp (UIA), Universiteitsplein 1, 2610 Antwerp (Wilrijk), Belgium
Geert Janssens
Affiliation:
Department of Animal Nutrition, Animal Genetics, Breeding and Ethology, Ghent University, Heidestraat 19, 9820 Merelbeke, Belgium
Micheline Van Caillie-Bertrand
Affiliation:
Department of Paediatric Gastroenterology and Nutrition, Koningin Paola Kinderziekenhuis, Algemeen Ziekenhuis Middelheim (AZM), Lindendreef 1, 2020 Antwerp, Belgium
Harry Robberecht
Affiliation:
Department of Pharmaceutical Sciences, Laboratory of Food Sciences, University of Antwerp (UIA), Universiteitsplein 1, 2610 Antwerp (Wilrijk), Belgium
Herman De Rycke
Affiliation:
Department of Animal Nutrition, Animal Genetics, Breeding and Ethology, Ghent University, Heidestraat 19, 9820 Merelbeke, Belgium
Roland De Wilde
Affiliation:
Department of Animal Nutrition, Animal Genetics, Breeding and Ethology, Ghent University, Heidestraat 19, 9820 Merelbeke, Belgium
Hendrik Deelstra
Affiliation:
Department of Pharmaceutical Sciences, Laboratory of Food Sciences, University of Antwerp (UIA), Universiteitsplein 1, 2610 Antwerp (Wilrijk), Belgium
*
*Corresponding author: Dr Douwina Bosscher, fax +32 3 820 27 34, email bosscher@uia.ua.ac.be
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Abstract

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An in vitro method was used to determine the availability of Zn from infant foods containing increasing amounts of phytate, and to quantify the effect of the phytate:Zn molar ratio on the availability. During the in vitro assay, digestive conditions of infants, younger and older than 4 months of age, were carefully simulated since the solubility of phytate–Zn complexes during digestion is pH dependent. Availability was measured with a continuous flow dialysis in vitro procedure with previous intralumen digestive stage. Zn concentrations were determined with flame atomic absorption spectrometry. Phytic acid content was measured with HPLC. Adding phytate to infant formula lowered Zn availability to 2·84 (SD 0·17) % WHEN THE PHYTATE:ZN MOLAR RATIO INCREASED TO 2·2 (P<0·05), AS COMPARED WITH COWS' MILK-BASED FORMULA (6·65 (sd 0·55) %). Availability from vegetables (23·83 (sd 2·17) %) significantly decreased (P<0·05) at a ratio > 7·9 (15·12 (sd 1·63) %). Zn availability from soyabean-based formula (2·26 (sd 0·36) %) was lower (P<0·05) compared with cows' milk-based formula (6·65 (sd 0·55) %). Availability between soyabean- and cows' milk-based formula was similar (P>0·05) when a phytate:Zn ratio of 2·2 (2·84 (sd 0·17) %) was obtained in the cows' milk formula. The negative effect of phytic acid on Zn availability was dependent on the type of the food and the phytate content, and should be considered when using soyabean-based formulas during early infancy.

Keywords

Bioavailability of micronutrientsZincPhytateInfantsIn vitro method
Type
Research Article
Information
British Journal of Nutrition , Volume 86 , Issue 2 , August 2001 , pp. 241 - 247
Copyright
Copyright © The Nutrition Society 2001

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