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The influence of rumen volatile fatty acids on protein metabolism in growing lambs

Published online by Cambridge University Press:  09 March 2007

H. A. Abdul–Razzaq  and
R. Bickerstaffe
H. A. Abdul–Razzaq
Affiliation:
Biochemistry Department, Lincoln College, Canterbury, New Zealand
R. Bickerstaffe
Affiliation:
Biochemistry Department, Lincoln College, Canterbury, New Zealand
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Abstract

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The effect of acetic or propionic acid rumen fermentation patterns on whole-body protein turnover, tissue protein synthetic rates and body composition was investigated in growing lambs. Protein turnover was assessed using a continuous intravenous infusion of [2,3-3H]tyrosine and tissue protein fractional synthetic rates (FSR) from the specific activities of plasma free, intracellular free and tissue bound tyrosine. Only the FSR of muscle tissue approached significance. The high FSR in the propionic group was attributed to the high plasma insulin concentration. Values for whole-body protein synthesis, corrected for tyrosine oxidation, were similar to those obtained by summating protein synthesis in individual tissues, confirming that tyrosine oxidation should be measured accurately if reliable whole-body protein synthesis values are required. Tyrosine oxidation and flux were high in the acetic acid group, suggesting that amino acids are used for gluconeogenesis. The high protein turnover rate probably ensures an adequate supply of gluconeogenic amino acids and that the penalty of mobilizing body proteins for gluconeogenic amino acids is minimal. In the propionic acid group, high plasma glucose and insulin concentrations were associated with a low protein turnover rate, high ratio of deposited: synthesized protein and a high body fat content. It is concluded that changing the proportion of ruminal volatile fatty acids influences protein turnover, protein synthesis and the efficiency of protein retention. Such factors probably contribute, indirectly, to the observed differences in body composition.

Keywords

Protein metabolismRumen VFAVolatile fatty acidsLamb
Type
Amino Acids and Proteins: Metabolism and Requirements
Information
British Journal of Nutrition , Volume 62 , Issue 2 , September 1989 , pp. 297 - 310
Copyright
Copyright © The Nutrition Society 1989

References

REFERENCES

Abdul-Razzaq, H. A. (1985). Influence of rumen volatile fatty acids and insulin on the metabolism of glucose and protein in lambs. PhD Thesis, Lincoln College, University of Canterbury, New Zealand.Google Scholar
Abdul-Razzaq, H.A., Bickerstaffe, R. & Savage, G.P. (1988). The influence of rumen volatile fatty acids on blood metabolites and body composition of growing lambs. Australian Journal of Agricultural Research 39, 505–515.CrossRefGoogle Scholar
Alpers, D.H. & Kinzie, J.L. (1973). Regulation of small intestinal protein metabolism. Gastroenterology 64, 471–496.CrossRefGoogle ScholarPubMed
Arnal, M. (1977). Muscle protein turnover in lambs throughout development. In Proceedings of the 2nd International Symposium on Protein Metabolism and Nutrition. European Association of Animal Production, Publication no. 22, pp. 35–37 [Tamminga, S., editor]. Wageningen, The Netherlands: Centre for Agricultural Publishing and Documentation.Google Scholar
Arnal, M., Ferrara, M. & Fauconneau, G. (1976). Protein synthesis in vivo during the development of various muscles in lambs. In Proceedings of an International Conference on Isotopes in Agriculture, pp. 393–401. Vienna, Austria: International Atomic Energy Agency.Google Scholar
Bassett, J.M. (1978). Endocrine factors in the control of nutrient utilization: ruminants. Proceedings of the Nutrition Society 37, 273–280.CrossRefGoogle ScholarPubMed
Bergman, E.N. & Heitmann, R.N. (1980). Metabolism of amino acids by the gut, liver, kidneys and peripheral tissues. In Protein Deposition in Animals, pp. 69–76. [Buttery, P. J. and Lindsay, D. B., editors]. London and Boston: Butterworths.CrossRefGoogle Scholar
Blaxter, K.L. (1961). Energy utilisation in the ruminants. In Digestive Physiology and Nutrition of the Ruminant, pp. 183–197 [Lewis, D., editor]. London: Butterworths.Google Scholar
Brockman, R.P. (1978). Roles of glucagon and insulin in the regulation of metabolism in ruminants—a review. Canadian Veterinary Journal 19, 55–62.Google Scholar
Bryant, D.T.W. & Smith, R.W. (1982 a). Protein synthesis in muscle of mature sheep. Journal of Agricultural Science, Cambridge 99, 319–323.CrossRefGoogle Scholar
Bryant, D. T. W. & Smith, R.W. (1982 b). The effect of lactation on protein synthesis in ovine skeletal muscle. Journal of Agricultural Science, Cambridge 98, 639–643.CrossRefGoogle Scholar
Buttery, P.J., Beckerton, A. & Lubbock, M.H. (1977). Rates of protein metabolism in sheep. In Proceedings of the 2nd International Symposium on Protein Metabolism and Nutrition. European Association of Animal Production, Publication no. 22, pp. 32–34. [Tamminga, S., editor]. Wageningen, The Netherlands: Centre for Agricultural Publishing and Documentation.Google Scholar
Buttery, P.J., Beckerton, A., Mitchell, R.M., Davies, K. & Annison, E.F. (1975). The turnover rate of muscle and liver protein in sheep. Proceedings of the Nutrition Society 34, 91A–92A.Google ScholarPubMed
Chambers, J.A.N. (1984). Glucose, protein and energy metabolism in suckling and ruminating lambs. PhD Thesis, Lincoln College, The University of Canterbury, New Zealand.Google Scholar
Chambers, J.A.N. & Bickerstaffe, R. (1982). Effect of rumen development on protein synthesis in lambs. Proceedings of the Nutrition Society Australia 7, 148A.Google Scholar
Chrystie, W.W., Horn, J., Sloan, I., Stern, M., Noakes, D. & Young, M. (1977). Effect of insulin on protein turnover in foetal lambs. Proceedings of the Nutrition Society 36, 118A.Google Scholar
Combe, E., Attaix, D. & Arnal, M. (1979). Protein turnover in the digestive tissues of the lamb throughout development. Annales des Recherches Vétérinaires 10, 436–439.Google ScholarPubMed
Davis, S.R., Barry, T.N. & Hughson, G.A. (1981). Protein synthesis in tissues of growing lambs. British Journal of Nutrition 46, 409–419.CrossRefGoogle ScholarPubMed
Eskeland, B., Pfander, W.H. & Preston, R.L. (1974). Intravenous energy infusions in lambs: effects on nitrogen retention, plasma free amino acids and plasma urea nitrogen. British Journal of Nutrition 31, 201–211.CrossRefGoogle ScholarPubMed
Fell, B.F., Campbell, R.M., Mackie, W.S. & Weekes, T. E. C. (1972). Changes associated with pregnancy and lactation in some extra-reproductive organs of the ewe. Journal of Agricultural Science, Cambridge 79, 397–407.CrossRefGoogle Scholar
Fulks, R.M., Li, J.B. & Goldberg, A.L. (1975). Effects of insulin, glucose and amino acids on protein turnover in rat diaphragm. Journal of Biological Chemistry 250, 290–298.CrossRefGoogle ScholarPubMed
Fuller, M.F., Reeds, P.J., Cadenhead, A., Seve, B. & Preston, T. (1987). Effects of the amount and quality of dietary protein on nitrogen metabolism and protein turnover of pigs. British Journal of Nutrition 58, 287–300.CrossRefGoogle ScholarPubMed
Garlick, P.J. & Marshall, I. (1972). A technique for measuring brain protein synthesis. Journal of Neurochemistry 19, 577–583.CrossRefGoogle ScholarPubMed
Garlick, P.J., Millward, D.J. & James, W. P. T. (1973). The diurnal response of muscle and liver protein synthesis in vivo in the meal-fed rats. Biochemical Journal 136, 935–945.CrossRefGoogle ScholarPubMed
Jefferson, L.S., Li, J.B. & Rannels, S.R. (1977). Regulation by insulin of amino acid release and protein turnover in the perfused rat hemicorpus. Journal of Biological Chemistry 252, 1476–1483.CrossRefGoogle ScholarPubMed
Knox, K.L. & Ward, G.M. (1961). Rumen concentrations of volatile fatty acids as affected by feeding frequency. Journal of Dairy Science 44, 1550–1553.CrossRefGoogle Scholar
Leng, R.A. & Ball, F.M. (1978). The role of glucose in growth. Proceedings of the Australian Society of Animal Production 12, 135A.Google Scholar
Lindsay, D.B. (1979). Is gluconeogenesis from amino acids important in ruminants? In Protein Metabolism in the Ruminant pp. 71–79. [Buttery, P.J., editor]. London: Agricultural Research Council.Google Scholar
Lindsay, D.B. (1981). Characteristics of the metabolism of carbohydrates in ruminants compared with other animals. In The Problem of Dark-cutting in Beef, pp. 101–113. [ Hood, D.E. and Tarrant, P.V., editors]. The Hague: Martinus Nijhoff.CrossRefGoogle Scholar
Lindsay, D.B. (1982). Relationship between amino acid catabolism and protein anabolism in the ruminant. Federation Proceedings 41, 2550–2554.Google ScholarPubMed
Lobley, G.E., Milne, V., Lovie, J.M., Reeds, P.J. & Pennie, K. (1980). Whole body and tissue protein synthesis in cattle. British Journal of Nutrition 43, 491–502.CrossRefGoogle ScholarPubMed
MacRae, J.C. & Egan, A.R. (1980). The measurements of glucose kinetics in sheep: what relevance do such measurements have to the glucose requirements of an animal? In Energy Metabolism pp. 421–426. [Mount, L.E., editor]. London and Boston: Butterworths.CrossRefGoogle Scholar
Moldawer, L.L., Kawamura, I., Bistrian, B.R. & Blackburn, G.L. (1983). The contribution of phenylalanine to tyrosine metabolism in vivo. Studies in the post-absorptive and phenylalanine-loaded rat. Biochemical Journal 210, 811–817.CrossRefGoogle ScholarPubMed
Morgan, H.E., Jefferson, L.S., Wolpert, E.B. & Rannels, D.E. (1971). Regulation of protein synthesis in heart muscle. Journal of Biological Chemistry 246, 2163–2170.CrossRefGoogle ScholarPubMed
Nicholas, G.A., Lobley, G.E. & Harris, C.I. (1977). Use of constant infusion technique for measuring rates of protein synthesis in New Zealand white rabbit. British Journal of Nutrition 38, 1–17.CrossRefGoogle ScholarPubMed
Oddy, V.H., Lindsay, D.B., Barker, P.J. & Northrop, A.J. (1987). Milk production in ewes fed high grain diets. British Journal of Nutrition 58, 437–452.CrossRefGoogle Scholar
Poole, D.A. & Allen, D.M. (1970). Utilization of salts of volatile fatty acids by growing sheep. 5. Effects of type of fermentation of the basal diet on the utilization of salts of acetic acid for body gains. British Journal of Nutrition 24, 695–704.CrossRefGoogle ScholarPubMed
Reeds, P.J., Cadenhead, A., Fuller, M.F., Lobley, G.E. & McDonald, J.D. (1980). Protein turnover in growing pigs. Effects of age and food intake. British Journal of Nutrition 43, 445–455.CrossRefGoogle ScholarPubMed
Rowe, J.B., Nolan, J.V. & Leng, R.A. (1978). Measurement of propionic acid and glucose metabolism using a modelling approach. Proceedings of the Australian Society of Animal Production 12, 136A.Google Scholar
Schreurs, V.V.A. M. & Boekholt, H.A. (1980). A study of the relative turnover of muscle proteins. European Association of Animal Production 27, 538–543.Google Scholar
Shipley, R.A. & Clark, R.E. (1972). Tracer Methods for in vivo Kinetics; Theory and Applications, pp. 239–246. New York: Academic Press.Google Scholar
Sinnett-Smith, P. A., Dumelow, N.W. & Buttery, P.J. (1983). Effects of trenbolone acetate and zeranol on protein metabolism in male castrate and female lambs. British Journal of Nutrition 50, 225–234.CrossRefGoogle ScholarPubMed
Soltesz, G., Joyce, J. & Young, M. (1973). Protein synthesis rate in the newborn lamb. Biology of the Neonate 23, 139–148.CrossRefGoogle ScholarPubMed
Stirewalt, W.S. & Low, R.B. (1983). Effects of insulin in vitro on protein turnover in rat epitrochlearis muscle. Biochemical Journal 210, 323–330.CrossRefGoogle ScholarPubMed
Van Es, A. J. H. (1980). Nutritional efficiency of protein and fat deposition. In Protein Deposition in Animals, pp. 215–224. [ Buttery, P.J. and Lindsay, D.B. editors]. London and Boston: Butterworths.CrossRefGoogle Scholar
Waalkes, T.P. & Udenfriend, S. (1957). A fluroimetric method for the estimation of tyrosine in plasma and tissues. Journal of Laboratory and Clinical Medicine 50, 733–766.Google Scholar
Wallace, L.R. (1948). The growth of lambs before and after birth in relation to the level of nutrition. Journal of Agricultural Science, Cambridge 38, 243–302.CrossRefGoogle Scholar
Waterlow, J.C., Garlick, P.J. & Millward, D.J. (1978). Protein Turnover in Mammalian Tissues and in the Whole Body. Amsterdam: North-Holland Publishing Company.Google Scholar
Wilson, S., MacRae, J.C. & Buttery, P.J. (1983). Glucose production and utilization in non-pregnant, pregnant and lactating ewes. British Journal of Nutrition 50, 303–316.CrossRefGoogle ScholarPubMed