Hostname: page-component-5c6d5d7d68-vt8vv Total loading time: 0.001 Render date: 2024-08-15T23:04:18.666Z Has data issue: false hasContentIssue false
  • English
  • Français

Levels of polyunsaturated fatty acids in lymphocytes of rats fed on diets varying in polyunsaturated fatty acid content

Published online by Cambridge University Press:  25 February 2008

W. M. Tsagn ,
J. Belin  and
A. D. Smith
W. M. Tsagn
Affiliation:
The Courtauld Institute of Biochemistry, The Middlesex Hospital Medical School, Mortimer Street, London, W1P 7PN
J. Belin
Affiliation:
The Courtauld Institute of Biochemistry, The Middlesex Hospital Medical School, Mortimer Street, London, W1P 7PN
A. D. Smith
Affiliation:
The Courtauld Institute of Biochemistry, The Middlesex Hospital Medical School, Mortimer Street, London, W1P 7PN
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. When weanling rats were fed on a diet containing 0.1 g/kg of the diet as polyunsaturated fatty acid, it was found that after 2 weeks the level of linoleate in the lymphocyte total lipids was 56 mg/ g total fatty acids, as compared with a level of 138 mg/ g in rats on a normal diet (P < 0.005). Similar levels were obtained from rats which had been fed for up to 16 weeks on the deficient diet, but in a group killed after 28 weeks on the diet the level was found to be only 20 mg/ g total fatty acids. The arachidonate level was found to be approximately 220 mg/ g total fatty acids, regardless of whether the rats were fed on a diet deficient in linoleate for up to 16 weeks or on a normal diet. In the group of rats killed after 28 weeks on the linoleate deficient diet, however, the arachidonate level was only 60 mg/ g total fatty acids.

2. Percentage values for total fatty acids are given for plasma, adipose tissue, and lymphocytes for rats on normal and experimental diets.

3. Scatter diagrams of the levels of linoleate v. arachidonate in the lymphocyte total fatty acids showed no correlation between the levels of the two acids (r 0.05), but similar plots of linoleate and oleate levels showed an inverse correlation (r – 0.68).

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 43 , Issue 2 , March 1980 , pp. 367 - 373
Copyright
Copyright © The Nutrition Society 1980

References

Awad, A. B. & Spector, A. A. (1976). Biochim. biophys. Acta 426, 723.CrossRefGoogle Scholar
Borowitz, M. J. & Blurn, J. J. (1976). Biochim. biophys. Acta 424, 114.CrossRefGoogle Scholar
Bayurn, A. (1968). Scand. J. clin. Lab. Invest. 21, 31, suppl. 97.Google Scholar
Chapman, D., Gomez-Fernandez, J. C. & Goni, F. M. (1979). FEBS Left. 98, 211.CrossRefGoogle Scholar
Clausen, J. & Møller, J. (1967). Acta neurol. scand. 43, 375.CrossRefGoogle Scholar
Engster, H. M., Carew, L. B. Jr., Harvey, S. & Scanes, C. G. (1979). J. Nutr. 109, 330.CrossRefGoogle Scholar
Farquhar, J. W. & Ahrens, E. H. Jr. (1963). J. din. Invest. 42, 675.Google Scholar
Ferguson, K. A., Glaser, M., Bayer, W. H., Vagelos, P. R. (1975). Biochemistry, Easton 14, 146.CrossRefGoogle Scholar
Folch, J., Lees, M. & Sloane-Stanley, G. H. (1956). J. biol. Chem. 226, 497.CrossRefGoogle Scholar
Guanieri, M. & Johnson, R. M. (1970). Adv. Lipid Res. 8, 115.CrossRefGoogle Scholar
Lenaz, G. (1979). The Role of Lipids in Membranes. Subcellular Biochemistry, vol. 6, ch. 5. [Roodyn, D. B., editor]. New York: Plenum Press.Google Scholar
Medawar, P. B., Hunt, R., Meade, C. J. & Mertin, J. (1975). Proc. Nutr. Soc. 35, 147A.Google Scholar
Mertin, J. & Hughes, D. (1975). Int. Archs Allergy appl. Immun. 48, 203.CrossRefGoogle Scholar
Mertin, J. & Hunt, R. (1976). Proc. nat. Acad. Sci. U.S.A. 73, 928.CrossRefGoogle Scholar
Mertin, J. & Stackpoole, A. (1979). Clin. exp. Immim. 36, 449.Google Scholar
Nugara, D. & Edwards, H. M. Jr. (1970). J. Nutr. 100, 539.CrossRefGoogle Scholar
Offner, H. & Clausen, J. (1975). Lancet ii, 1204.Google Scholar
Parker, C. W., Stenson, W. F., Huber, M. G. & Kelly, J. P. (1979). J. Immun. 122, 1572.CrossRefGoogle Scholar
Rintoul, D. A., Sklar, L. A. & Sirnoni, R. D. (1978). J. biol. Chem. 253, 7447.CrossRefGoogle Scholar
Santiago-Delpin, E. A. & Szepsenwol, J. (1977). J. natn. Cancer Inst. 59, 459.CrossRefGoogle Scholar
Selivonchick, D. P. & Johnston, V. P. (1975). J. Nutr. 105, 288.CrossRefGoogle Scholar
Tsai, P. & Geyer, R. P. (1978). J. biol. Chem. 253, 5087.CrossRefGoogle Scholar
Tsang, W. M., Weyrnan, C. & Smith, A. D. (1977). Biochem. Soc. Trans. 5, 153.CrossRefGoogle Scholar
Weyrnan, C., Morgan, S., Relin, J. & Smith, A. D. (1977). Biochim. biophys. Acfa 496, 155.Google Scholar
Yu, B. P., Kurnmerow, F. A. & Nishida, T. (1966). J. Nutr. 89, 435.Google Scholar