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Preparation of an experimental low-fluoride diet from single-cell organisms for rats and mice

Published online by Cambridge University Press:  09 March 2007

S. A. Khalawan ,
J. C. Elliott  and
R. W. Fearnhead
S. A. Khalawan
Affiliation:
Department of Dental Anatomy, The London Hospital Medical College, Turner Street, London E1 2AD
J. C. Elliott
Affiliation:
Department of Dental Anatomy, The London Hospital Medical College, Turner Street, London E1 2AD
R. W. Fearnhead
Affiliation:
Department of Dental Anatomy, The London Hospital Medical College, Turner Street, London E1 2AD
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Abstract

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1. A method for producing a standard low-fluoride diet from a green alga and yeast is described. Chlorella pyrenoidosa was grown in a culture medium prepared with distilled water and analytical grade chemical salts. The spent culture medium from the alga culture was reclaimed and replenished with salts and sucrose for the production of yeast, Saccharomyces cerevisiae.

2. The single-cell organisms were separated by centrifugation from their culture media and the dried cells were blended with sucrose, maize oil, cellulose and a salt mix to produce diet pellets for rats and mice.

3. The diet was readily accepted as food by rats and mice and it was found to contain 100–300 μg fluoride/kg dry weight. Two generations of rats and four generations of mice were bred on this diet.

4. The use of hydroxyapatite to reduce the fluoride content of the chemicals used in the production of the alga and yeast biomass was investigated. Diet pellets prepared with this biomass contained 45–60 μg fluoride/kg dry weight.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 44 , Issue 3 , November 1980 , pp. 371 - 380
Copyright
Copyright © The Nutrition Society 1980

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