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Relation of antigenic structure of cereal proteins to their toxicity in coeliac patients

Published online by Cambridge University Press:  07 March 2008

P. J. Ciclitira ,
H. J. Ellis ,
D. J. Evans  and
E. S. Lennox
P. J. Ciclitira
Affiliation:
Gastroenterology Unit, Department of Medicine, Guy's Hospital, United Medical School of Guy's and St Thomas' Hospitals, London SE1 9RT
H. J. Ellis
Affiliation:
Gastroenterology Unit, Department of Medicine, Guy's Hospital, United Medical School of Guy's and St Thomas' Hospitals, London SE1 9RT
D. J. Evans
Affiliation:
Department of Histopathology, Hammersmith Hospital, LondonW12 0HS
E. S. Lennox
Affiliation:
MRC Laboratory of Molecular Biology, CambridgeCB2 2QH
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Abstract

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1. Unfractionated gliadin and its α-, β-, γ- and ω-gliadin subfractions were used as rabbit immunogens. The antisera were characterized by (1) Ouchterlony double diffusion, (2) binding of 125I-labelled gliadin subfractions, (3) inhibition by several gliadin subfractions of binding between γgliadin antiserum and 125I-labeIled γgliadin.

2. Double diffusion showed identical cross-reactivity between the antisera and the gliadin subfractions with the exception of ω-gliadin. Precipitin lines of partial identity with gliadin were observed against rye secalins and barley hordeins but not oat avenins or maize zeins.

3. Binding was observed between unfractionated 125I-labelledα-, β-, γ- and ω-gliadins and all the antisera. There was binding of 125I-labelled ω-gliadin to the ω-gliadin antiserum but poor binding of 125I-labelled ω-gliadin to unfractionated α-, β- and γ-gliadin antisera. Competitive inhibition of binding between 125I-labelled γ-gliadin and γ-gliadin antiserum diluted 1:250 (v/v) demonstrated similar competition between α-, β- and γ-gliadins and this antiserum but poor competition between ω-gliadin, wheat glutenins, albumins and globulins, rye secalins, barley hordeins and oat avenins.

4. These findings suggest that there is a good correlation between the antigenic structure of gliadin proteins and their toxicity to patients with coeliac disease.

Type
Papers of direct relevance to Clinical and Human Nutrition
Information
British Journal of Nutrition , Volume 53 , Issue 1 , January 1985 , pp. 39 - 45
Copyright
Copyright © The Nutrition Society 1985

References

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