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T-lymphocyte subsets and interleukin-2 production in zinc-deficient rats

Published online by Cambridge University Press:  09 March 2007

Pauline S. Dowd ,
J. Kelleher.  and
P. J. Guillou
Pauline S. Dowd
Affiliation:
University Department of Surgery, St James's University Hospital, Leeds LS9 7TF
J. Kelleher.
Affiliation:
University Department of Surgery, St James's University Hospital, Leeds LS9 7TF
P. J. Guillou
Affiliation:
University Department of Surgery, St James's University Hospital, Leeds LS9 7TF
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Abstract

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1. It has been suggested that zinc-deficiency impairs cellular (T-lymphocyte-mediated) immune responses via a selective effect on helper T-lymphocytes. We have addressed this question in the rat by employing recently developed reagents in the form of monoclonal antibodies which specifically identify rat T-lymphocyte subsets (identifying total T-cells, helper T-cells and suppressor T-cells) and also by quantifying helper T-cell function by measurement of the helper T-cell-derived molecule interleukin-2 (IL-2).

2. Zn-deficiency induced T-cell atrophy (assessed morphologically and phenotypically with anti-rat T-cell monoclonal antibodies) in both peripheral blood and spleen. The use of these specific monoclonal antibodies failed to demonstrate a selective effect of Zn deficiency on the helper T-cell fraction of the total T-lymphocyte population.

3. In contrast, the results of functional assays of the T-lymphocyte response were dependent on the conditions of culture but suggested that the generation of IL-2 and its corresponding receptor were determined by the intracellular Zn status. Thus, in vivo, helper T-lymphocyte numbers are non-specifically reduced since other T-cell subsets are also reduced in response to appropriate stimulation. The functional consequences of this are dependent on the intracellular concentration of Zn but appear to influence both IL-2 production and its receptors on activated T-cells.

Type
Papers of direct relevance to Clinical and Human Nutrition
Information
British Journal of Nutrition , Volume 55 , Issue 1 , January 1986 , pp. 59 - 69
Copyright
Copyright © The Nutrition Society 1986

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