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Vegetable lipid sources in vitro biosyntheis of triacylglycerols and phospholipids in the intestine of sea bream (Sparus aurata)

Published online by Cambridge University Press:  08 March 2007

Maria José Caballero ,
Germ´an Gallardo ,
Lidia Robaina ,
Daniel Montero ,
Antonio Fernández  and
Marisol Izquierdo
Maria José Caballero*
Affiliation:
Department of Comparative Pathology, Trasmontaña, s/n, 35416 Arucas, Las Palmas de Gran Canaria, Canary Islands, Spain
Germ´an Gallardo
Affiliation:
Department of Biochemical, Cellular Biology and Physiology, Edificio Ciencias de la Salud, Campus Universitario de San Cristóbal, Las Palmas de Gran Canaria, Canary IslandsSpain
Lidia Robaina
Affiliation:
Grupo de Investigación en Acuicultura, ULPGC & ICCM, PO Box 56, 35200, Telde, Las Palmas de Gran Canaria, Canary Islands, Spain
Daniel Montero
Affiliation:
Grupo de Investigación en Acuicultura, ULPGC & ICCM, PO Box 56, 35200, Telde, Las Palmas de Gran Canaria, Canary Islands, Spain
Antonio Fernández
Affiliation:
Department of Comparative Pathology, Trasmontaña, s/n, 35416 Arucas, Las Palmas de Gran Canaria, Canary Islands, Spain
Marisol Izquierdo
Affiliation:
Grupo de Investigación en Acuicultura, ULPGC & ICCM, PO Box 56, 35200, Telde, Las Palmas de Gran Canaria, Canary Islands, Spain
*
*Corresponding authorfax +34 928451141, email mcaballero@dmor.ulpgc.es
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Abstract

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Despite the good growth performance of several fish species when dietary fish oil is partly replaced by vegetable oils, recent studies have reported several types of intestinal morphological alterations in cultured fish fed high contents of vegetable lipid sources. However, the physiological process implied in these morphological changes have not been clarified yet, since alterations in the physiological mechanisms involved in the different processes of lipid absorption could be responsible for such gut morphological features. The objective of the present study was to investigate the activities of reacylation pathways in fish, the glycerol-3-phosphate and the monoacylglycerol pathways, in order to clarify the intestinal triacylglycerol (TAG) and phospholipid biosynthesis to better understand the morphological alterations observed in the intestine of fish fed vegetable oils. Intestinal microsomes of sea bream fed different lipid sources (fish, soyabean and rapeseed oils) at three different inclusion levels were isolated and incubated with l-[14C(U)]glycerol-3-phosphate and [1-14C]palmitoyl CoA. The results showed that in this fish species the glycerol-3-phosphate pathway is mainly involved in phospholipid synthesis, whereas TAG synthesis is mainly mediated by the monoacylglycerol pathway. Feeding with rapeseed oil reduced the reacylation activity in both pathways, explaining the high accumulation of lipid droplets in the supranuclear portion of the intestinal epithelium, whereas soyabean oil enhanced phosphatidylcholine synthesis, being associated with the increase in VLDL found in previous studies.

Keywords

Intestinal microsomesVegetable oilsSea breamMonoacylglycerol pathwayGlycerol-3-phosphate pathwayCytidine diphosphate nucleotidesTriacylglycerolsPhospholipids
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 3 , March 2006 , pp. 448 - 454
Copyright
Copyright © The Nutrition Society 2006

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