Hostname: page-component-77c89778f8-5wvtr Total loading time: 0 Render date: 2024-07-22T06:03:09.588Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of insecticides chlorpyrifos, emamectin benzoate and fipronil on Spodoptera litura might be mediated by OBPs and CSPs

Published online by Cambridge University Press:  04 December 2017

X. Lin ,
Y. Jiang ,
L. Zhang  and
Y. Cai
X. Lin*
Affiliation:
College of Life Sciences, China Jiliang University, Hangzhou 310018, China
Y. Jiang
Affiliation:
College of Life Sciences, China Jiliang University, Hangzhou 310018, China
L. Zhang
Affiliation:
College of Life Sciences, China Jiliang University, Hangzhou 310018, China
Y. Cai
Affiliation:
College of Life Sciences, China Jiliang University, Hangzhou 310018, China
*
*Author for correspondence Phone: +86-139-5802-8822 Fax: +86-571-86914449 E-mail: linxinda@cjlu.edu.cn
Get access Rights & Permissions [Opens in a new window]

Abstract

Spodoptera litura is a widespread polyphagous insect pest that can develop resistance and cross-resistance to insecticides, making it difficult to control. Insecticide exposure has previously been linked with induction of specific olfactory-related proteins, including some chemosensory proteins (CSPs) and odorant-binding proteins (OPBs), which may disrupt detection of environmental factors and reduce fitness. However, functional evidence supporting insecticide and OBPs/CSPs mediation remains unknown. Here we fed male S. litura moths with sucrose water containing one of three insecticides, chlorpyrifos, emamectin benzoate or fipronil, and used real-time quantitative polymerase chain reaction and RNAi to investigate OBPs and CSPs expression and their correlations with survival. Chlorpyrifos and emamectin benzoate increased expression of 78% of OBPs, plus 63 and 56% of CSP genes, respectively, indicating a major impact on these gene families. RNAi knockdown of SlituCSP18, followed by feeding with chlorpyrifos or fipronil, decreased survival rates of male moths significantly compared with controls. Survival rate also decreased significantly with the downregulation of SlituOBP9 followed by feeding with chlorpyrifos. Thus, although these three insecticides had different effects on OBP and CSP gene expression, we hypothesize that SlituOBPs and SlituCSPs might mediate their effects by increasing their expression levels to improve survival. Moreover, the differential response of S. litura male moths to the three insecticides indicated the potential specificity of chlorpyrifos affect SlituCSP18 and SlituOBP9 expression.

Keywords

Spodoptera liturachlorpyrifosemamectin benzoatefipronil
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 108 , Issue 5 , October 2018 , pp. 658 - 666
Check for updates
Copyright
Copyright © Cambridge University Press 2017 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ahmad, M. & Mehmood, R. (2015) Monitoring of resistance to new chemistry insecticides in Spodoptera litura (Lepidoptera: Noctuidae) in Pakistan. Journal of Economic Entomology 108, 1279–1288.Google Scholar
Ahmad, M., Sayyed, A.H., Saleem, M.A. & Ahmad, M. (2008) Evidence for field evolved resistance to newer insecticides in Spodoptera litura (Lepidoptera: Noctuidae) from Pakistan. Crop Protection 27, 1367–1372.Google Scholar
Angeli, S., Ceron, F., Scaloni, A., Monti, M., Monteforti, G., Minnocci, A., Petacchi, R. & Pelosi, P. (2001) Purification, structural characterization, cloning and immunocytochemical localization of chemoreception proteins from Schistocerca gregaria. European Journal of Biochemistry 262, 745–754.Google Scholar
Bautista, M.A., Bhandary, B., Wijeratne, A.J., Michel, A.P., Hoy, C.W. & Mittapalli, O. (2015) Evidence for trade-offs in detoxification and chemosensation gene signatures in Plutella xylostella. Pest Management Science 71, 423–432.Google Scholar
Brogdon, W.G. & McAllister, J.C. (1999) Insecticide resistance and vector control. Journal of Agromedicine 6, 41–58.Google Scholar
El-Aswad, A.F., Abdelgaleil, S.A. & Nakatani, M. (2004) Feeding deterrent and growth inhibitory properties of limonoids from Khaya senegalensis against the cotton leafworm, Spodoptera littoralis. Pest Management Science 60, 199–203.Google Scholar
Feng, B., Lin, X., Zheng, K., Qian, K., Chang, Y. & Du, Y. (2015) Transcriptome and expression profiling analysis link patterns of gene expression to antennal responses in Spodoptera litura. BMC Genomics 16, 269.Google Scholar
Field, L.M., Pickett, J.A. & Wadhams, L.J. (2000) Molecular studies in insect olfaction. Insect Molecular Biology 9, 545–551.Google Scholar
Gant, D.B., Chalmers, A.E., Wolff, M.A., Hoffman, H.B., Bushey, D.F., Kuhr, R.J. & Motoyama, N. (1998) Fipronil: action at the GABA receptor, pp. 147–156.Google Scholar
Gong, L., Luo, Q. & Hu, R.H.M.Y. (2012) Cloning and characterization of three chemosensory proteins from Spodoptera exigua and effects of gene silencing on female survival and reproduction. Bulletin of Entomological Research 102, 600–609.Google Scholar
Guo, X.L., Ning, X., Dong, C., Hong, Y.X., Zhong, X.F., Yu, P.B., Picimbon, J.F., Yu, C.Q., Su, T.Z. & Yao, F.L. (2014) Biotype expression and insecticide response of Bemisia tabaci chemosensory protein-1. Archives of Insect Biochemistry & Physiology 85, 137–151.Google Scholar
Haynes, K.F. (1988) Sublethal effects of neurotoxic insecticides on insect behavior. Annual Review of Entomology 33, 149–168.Google Scholar
Ikeda, T., Nagata, K., Kono, Y., Yeh, J.Z. & Narahashi, D.T. (2004) Fipronil modulation of GABA A receptor single-channel currents. Pest Management Science 60, 487–492.Google Scholar
Ishaaya, I., Kontsedalov, S. & Horowitz, A.R. (2002) Emamectin, a novel insecticide for controlling field crop pests. Pest Management Science 58, 1091–1095.Google Scholar
Keil, T.A. & Steiner, C. (1991) Morphogenesis of the antenna of the male silkmoth. Antheraea polyphemus, III. Development of olfactory sensilla and the properties of hair-forming cells. Tissue & Cell 23, 821–851.Google Scholar
Kranthi, K.R., Jadhav, D.R., Kranthi, S., Wanjari, R.R., Ali, S.S. & Russell, D.A. (2002) Insecticide resistance in five major insect pests of cotton in India. Crop Protection 21, 449–460.Google Scholar
López, J.D., Latheef, M.A. & Hoffmann, W.C. (2010) Effect of emamectin benzoate on mortality, proboscis extension, gustation and reproduction of the corn earworm, Helicoverpa zea. Journal of Insect Science 10, 1101–1113.Google Scholar
Liang, P., Guo, Y., Zhou, X. & Gao, X. (2014) Expression profiling in Bemisia tabaci under insecticide treatment: indicating the necessity for custom reference gene selection. PLoS ONE 9, e87514.Google Scholar
Liu, G., Ma, H., Xie, H., Xuan, N., Guo, X., Fan, Z., Rajashekar, B., Arnaud, P., Offmann, B. & Picimbon, J.F. (2016) Biotype characterization, developmental profiling, insecticide response and binding property of Bemisia tabaci chemosensory proteins: role of CSP in insect defense. PLoS ONE 11, e0154706.Google Scholar
Liu, G.X., Xuan, N., Chu, D., Xie, H.Y., Fan, Z.X., Bi, Y.P., Picimbon, J.-F., Qin, Y.C., Zhong, S.T., Fa Li, Y., Gao, Z.L., Pan, W.L., Wang, G.Y. & Rajashekar, B. (2014) Biotype expression and insecticide response of Bemisia tabaci chemosensory protein-1. Archives of Insect Biochemistry and Physiology 85, 137–151.Google Scholar
Livak, K.J. & Schmittgen, T.D. (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods 25, 402–408.Google Scholar
Lockwood, J.A., Sparks, T.C. & Story, R.N. (1983) Evolution of insect resistance to insecticides: a reevaluation of the roles of physiology and behavior. Bulletin of the Entomological Society of America 30, 41–51.Google Scholar
Lu, Y., Yuan, M., Gao, X., Kang, T., Zhan, S., Hu, W. & Li, J. (2013) Identification and validation of reference genes for gene expression analysis using quantitative PCR in Spodoptera litura (Lepidoptera: Noctuidae). PLoS ONE 8, e68059.Google Scholar
Maleszka, J., Forêt, S., Saint, R. & Maleszka, R. (2007) RNAi-induced phenotypes suggest a novel role for a chemosensory protein CSP5 in the development of embryonic integument in the honeybee (Apis mellifera). Development Genes & Evolution 217, 189–196.Google Scholar
Ozaki, M., Wadakatsumata, A., Fujikawa, K., Iwasaki, M., Yokohari, F., Satoji, Y., Nisimura, T. & Yamaoka, R. (2005) Ant nestmate and non-nestmate discrimination by a chemosensory sensillum. Science 309, 311–314.Google Scholar
Pelosi, P., Iovinella, I., Zhu, J., Wang, G. & Dani, F.R. (2017) Beyond chemoreception: diverse tasks of soluble olfactory proteins in insects. Biological Reviews, doi:10.1111/brv.12339.Google Scholar
Picimbon, J.F. (2003) 18–Biochemistry and evolution of OBP and CSP proteins. Insect Pheromone Biochemistry & Molecular Biology, pp. 539–566.Google Scholar
Rehan, A. & Freed, S. (2014) Selection, mechanism, cross resistance and stability of spinosad resistance in Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). Crop Protection 56, 10–15.Google Scholar
Sakurai, T., Mitsuno, H., Haupt, S.S., Uchino, K., Yokohari, F., Nishioka, T., Kobayashi, I., Sezutsu, H., Tamura, T. & Kanzaki, R. (2011) A single sex pheromone receptor determines chemical response specificity of sexual behavior in the silkmoth Bombyx mori. PLoS Genetics 7, 171–177.Google Scholar
Sayyed, A.H., Ahmad, M. & Saleem, M.A. (2008) Cross-resistance and genetics of resistance to indoxacarb in Spodoptera litura (Lepidoptera: Noctuidae). Journal of Economic Entomology 101, 472–479.Google Scholar
Steinbrecht, R.A. (1998) Odorant-binding proteins: expression and function. Annals of the New York Academy of Sciences 855, 323–332.Google Scholar
Tang, W.C., Sun, H.X., Shu, Y.H., Zhou, Q. & Zhang, G.R. (2009) Effects of low concentrations of chlorpyrifos on development and food utilization of Spodoptera litura Fabricius (Lepidoptera: Noctuidae). Journal of Environmental Entomology 31, 137–142.Google Scholar
Tricoire-Leignel, H., Thany, S.H., Gadenne, C. & Anton, S. (2012) Pest insect olfaction in an insecticide-contaminated environment: info-disruption or hormesis effect. Frontiers in Physiology 3, 76–81.Google Scholar
Xu, G.-Z., You, Y.-W. & Zhang, L. (2015) A review on chemosensory protein and its functions in insects. Journal of Agricultural Biotechnology 23, 8.Google Scholar
Xuan, N., Guo, X., Xie, H.Y., Lou, Q.N., Lu, X.B., Liu, G.X. & Picimbon, J.F. (2014) Increased expression of CSP and CYP genes in adult silkworm females exposed to avermectins. Insect Science 22, 203–219.Google Scholar
Zhang, Y.N., Zhu, X.Y., Fang, L.P., He, P., Wang, Z.Q., Chen, G., Sun, L., Ye, Z.F., Deng, D.G. & Li, J.B. (2015) Identification and expression profiles of sex pheromone biosynthesis and transport related genes in Spodoptera litura. PLoS ONE 10, e0140019.Google Scholar