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Examination of the isolated autosomes of the SKA strain of house-flies (Musca domestica L.) for resistance to several insecticides with and without pretreatment with sesamex and TBTP

Published online by Cambridge University Press:  10 July 2009

R. M. Sawicki  and
A. W. Farnham
R. M. Sawicki
Affiliation:
Rothamsted Experimental Station, Harpenden, Herts.
A. W. Farnham
Affiliation:
Rothamsted Experimental Station, Harpenden, Herts.
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Extract

The factors of resistance to many insecticides were located and isolated in a homozygous condition from the diazinon-selected SKA strain of house-flies (Musca domestica L.) by inbreeding each of the five autosomes of the SKA strain into a susceptible multi-marker strain ac;ar;bwb;ocra SRS. The insecticides were: DDT, methoxychlor, tri-butyl tin, the methoxy and ethoxy analogues of parathion, chlorthion and malathion and their corresponding phosphates, diazinon and diazoxon.

The SKA flies' autosomes had the following factors of resistance:—autosome I—very slight resistance to ethyl-chlorthion; autosome II—gene a for low ali-esterase activity which conferred weak to moderate resistance against all organophosphates tested, and Deh (DDT-dehydrochlorinase) which gave great resistance to DDT, but none against methoxychlor; autosome III—Pen, which delayed knock-down by slowing down the entry of insecticides into the flies and had a negligible effect at death except against tri-butyl tin and methoxychlor, and a factor of resistance to Zectran unaffected by sesamex; autosome IV—Dld, the major factor of resistance to dieldrin; and autosome V—Ses, a sesamex-suppressed factor, which gave weak to moderate resistance against ethyl-malaoxon, diazoxon, diazinon, DDT and methoxychlor, but was ineffective against the other organophosphates tested.

TBTP (S,S,S tri-butyl phosphorotrithioate), an ali-esterase inhibitor, greatly synergised organophosphates only against insects with gene a. Pretreatment with sesamex, an inhibitor of microsomal activity, elicited two types of response: antagonism with most of the thioates, most pronounced in flies with gene a, and synergism with the phosphates, especially evident with flies with factor Ses. The possible reasons for differences in the response to organophosphates after pretreatment with TBTP or sesamex, and the nature of the resistance factors are discussed. High resistance in SKA flies against organophosphates arises through the interaction of resistance factors which singly give only weak to moderate resistance.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 59 , Issue 3 , December 1969 , pp. 409 - 421
Copyright
Copyright © Cambridge University Press 1969

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References

van Asperen, K. (1962). A study of housefly esterases by means of a sensitive colorimetric method.—J. Insect Physiol. 8, 401–416.CrossRefGoogle Scholar
Bell, J. D. (1968 a). Patterns of cross resistance to organophosphates and carbamates in Musca domestica.—Bull. ent. Res. 58, 137–151.CrossRefGoogle Scholar
Bell, J. D. (1968 b). Genetical investigations on a strain of houseflies resistant to organophosphates and carbamates.—Bull. ent. Res. 58, 191–200.CrossRefGoogle Scholar
Busvine, J. R. (1959). Patterns of insecticide resistance to organophosphorus compounds in strains of houseflies from various sources.—Entomologia exp. appl. 2, 58–67.CrossRefGoogle Scholar
El Basheir, S. & Sawicki, R. M. (1964). Cross-resistance of the SKA strain of housefly.—Rep. Rothamsted exp. Stn 1963, 129–130.Google Scholar
Franco, M. G. & Oppenoorth, F. J. (1962). Genetical experiments on the gene for low aliesterase activity and organophosphate resistance in Musca domestica L.—Entomologia exp. appl. 5, 119–123.CrossRefGoogle Scholar
Georghiou, G. P. (1965). Genetic studies on insecticide resistance.—Adv. Pest Control Res. 6, 171–230.Google ScholarPubMed
Gil, L., Fine, B. C, Dinamarca, M. L., Balazs, I., Busvine, J. R. & Agosin, M. (1968). Biochemical studies on insecticide resistance in Musca domestica.—Entomologia exp. appl. 11, 15–29.CrossRefGoogle Scholar
Hoyer, R. F. & Plapp, F. W. jr. (1966). A gross genetic analysis of two DDT-resistant housefly strains.—J. econ. Ent. 59, 495–501.CrossRefGoogle Scholar
Hoyer, R. F. & Plapp, F. W. jr. (1968). Insecticide resistance in the house fly: identification of a gene that confers resistance to organotin insecticides and acts as an intensifier of parathion resistance.—J. econ. Ent. 61, 1269–1276.CrossRefGoogle ScholarPubMed
Hoyer, R. F., Plapp, F. W. jr., & Orchard, R. D. (1965). Linkage relationships of several insecticide factors in the house fly (Musca domestica L.).—Entomologia exp. appl. 8, 65–73.CrossRefGoogle Scholar
Lewis, J. (1969). Metabolism of organophosphorus insecticides by strains of housefly (Musca domestica).—Rep. Rothamsted exp. Stn 1968 pt. 1, 171–172.Google Scholar
Lichtwardt, E. T. (1964). A mutant linked to the DDT-resistance of an Illinois strain of house flies.—Entomologia exp. appl. 7, 296–309.CrossRefGoogle Scholar
Milani, R. & Franco, M. G. (1959). Compartamento ereditario della resistenza al DDT in incroci tra il ceppo Orlando-R e ceppi Kdr e Kdr+ di Musca domestica L.—Symp. genet. 6, 269–303.Google Scholar
Nakatsugawa, T. & Dahm, P. A. (1967). Microsomal metabolism of parathion.—Biochem. Pharmac. 16, 25.CrossRefGoogle Scholar
Nguy, V. D. & Busvine, J. R. (1960). Studies of the genetics of resistance to parathion and malathion in the housefly.—Bull. Wld Hlth Org. 22, 531–542.Google ScholarPubMed
Ogita, Z. & Kasai, T. (1965). A genetic analysis of synergistic action of sulfonamide derivatives with DDT against houseflies.—Botyu-Kagaku 30, 119–128.Google Scholar
Oppenoorth, F. J. (1959). Genetics of resistance to organophosphorus compounds and low ali-esterase activity in the housefly.—Entomologia exp. appl. 2, 304–319.CrossRefGoogle Scholar
Oppenoorth, F. J. (1965 a). DDT-resistance in the housefly dependent on different mechanisms and the action of synergists.—Meded. LandbHoogesch. OpzoekStns Gent 30, 1390–1396.Google Scholar
Oppenoorth, F. J. (1965 b). Some cases of resistance caused by the alteration of enzymes.—Proc. XII int. Congr. Ent. 1964, 240–242.Google Scholar
Oppenoorth, F. J. & van Asperen, K. (1961). The detoxication enzymes causing organophosphate resistance in the housefly; properties, inhibition, and the action of inhibitors as synergists.—Entomologia exp. appl. 4, 311–333.CrossRefGoogle Scholar
Oppenoorth, F. J. & Houx, N. W. H. (1968). DDT resistance in the housefly caused by microsomal degradation.—Entomologia exp. appl. 11, 81–93.CrossRefGoogle Scholar
Oppenoorth, F. J. & Nasrat, G. E. (1966). Genetics of dieldrin and γ-BHC resistance in the housefly.—Entomologia exp. appl. 9, 223–231.CrossRefGoogle Scholar
Plapp, F. W. jr., Bigley, W. S., Chapman, G. A. & Eddy, G. W. (1963). Synergism of malathion against resistant house flies and mosquitoes.—J. econ. Ent. 56, 643–649.CrossRefGoogle Scholar
Plapp, F. W. jr. & Hoyer, R. F. (1967). Insecticide resistance in the house fly: resistance spectra and preliminary genetics of resistance in eight strains.—J. econ. Ent. 60, 768–774.CrossRefGoogle ScholarPubMed
Plapp, F. W. jr. & Hoyer, R. F. (1968). Insecticide resistance in the house fly: decreased rate of absorption as the mechanism of action of a gene that acts as an intensifier of resistance.—J. econ. Ent. 61, 1298–1303.Google ScholarPubMed
Sawicki, R. M. & Farnham, A. W. (1964). A dipping technique for selecting house-flies, Musca domestica L., for resistance to insecticides.—Bull. ent. Res. 55, 541–546.CrossRefGoogle Scholar
Sawicki, R. M. & Farnham, A. W. (1967 a). Genetics of resistance to insecticides of the SKA strain of Musca domestica I. Location of the main factors responsible for the maintenance of high DDT-resistance in diazinon-selected SKA flies.—Entomologia exp. appl. 10, 253–262.CrossRefGoogle Scholar
Sawicki, R. M. & Farnham, A. W. (1967 b). Genetics of resistance to insecticides of the SKA strain of Musca domestica II. Isolation of the dominant factors of resistance to diazinon.—Entomologia exp. appl. 10, 363–376.CrossRefGoogle Scholar
Sawicki, R. M. & Farnham, A. W. (1968). Genetics of resistance to insecticides of the SKA strain of Musca domestica III. Location and isolation of the factors of resistance to dieldrin.—Entomologia exp. appl. 11, 133–142.CrossRefGoogle Scholar
Sawicki, R. M. & Farnham, A. W. (1969). Interaction between the factors of resistance.—Rep. Rothamsted exp. Stn 1968 pt. 1, 173–174.Google Scholar
Sawicki, R. M., Franco, M. G. & Milani, R. (1966). Genetic analysis of non-recessive factors of resistance to diazinon in the SKA train of the housefly (Musca domestica L.).—Bull. Wld Hlth Org. 35, 893–903.Google Scholar
Sun, Y. P. & Johnson, E. R. (1960). Synergistic and antagonistic actions of insecticide-synergist combinations and their mode of action.—J. agric. Fd Chem. 8, 261–266.CrossRefGoogle Scholar
Tsukamoto, M. (1964). Methods for the linkage-group determination of insecticide-resistance factors in the housefly.—Botyu-Kagaku 29, 51–59.Google Scholar
Tsukamoto, M. & Suzuki, R. (1964). Genetic analyses of DDT-resistance in two strains of the housefly, Musca domestica L.—Botyu-Kagaku 29, 76–89.Google Scholar
Tsukamoto, M. & Suzuki, R. (1966). Genetic analyses of diazinon-resistance in the housefly.—Botyu-Kagaku 31, 1–14.Google Scholar
Wagoner, D. E. (1967). Linkage group—karyotype correlation in the house fly determined by cytological analysis of X-ray induced translocations.—Genetics, Princeton 57, 729–739.CrossRefGoogle ScholarPubMed
Wilkinson, C. F. (1968). Detoxication of pesticides and the mechanism of synergism.—In Hodgson, E. Ed. Enzymetic oxidation of toxicants.—229 pp. North Carolina St. Univ.Google Scholar