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Monitoring frequencies of insecticide resistance in Myzus persicae (Sulzer) (Hemiptera: Aphididae) in England during 1985–86 by immunoassay

Published online by Cambridge University Press:  10 July 2009

R. H. Ffrenchconstant  and
A. L. DEVONSHIRE
R. H. Ffrenchconstant
Affiliation:
Rothamsted Experimental Station, Harpenden, Herts., AL5 2JQ, UK
A. L. DEVONSHIRE*
Affiliation:
Rothamsted Experimental Station, Harpenden, Herts., AL5 2JQ, UK
*
†To whom all correspondence should be sent.
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Abstract

An immunoassay for the carboxylesterase E4, which confers insecticide resistance in Myzus persicae (Sulzer), was used to monitor the distributions of E4 activity within samples comprising more than 5000 aphids from sites in eastern and southern England. The quantitative precision of this assay is an improvement on the subjective assessment of E4 band intensity on electrophoresis gels previously used. Activity distributions did not always show discrete components corresponding to S, R1, R2 or R3 variants and thus, though convenient, this classification should be used cautiously. Distributions derived by immunoassay of many insects from the field are best compared to those of laboratory-reared standards classified according to E4 quantity (V1-V64). Data from untreated crops are presented in this way and discussed in the light of previous surveys. A large proportion of aphids corresponding to the V8 variant (intermediate between ‘R1’ and ‘R2’ categories) were found, and frequencies of resistance differed on both a local and regional scale. The majority of aphids from treated crops had E4 activities equivalent to, or above, that of V16 (i.e. ‘R2’).

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 78 , Issue 1 , March 1988 , pp. 163 - 171
Copyright
Copyright © Cambridge University Press 1988

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References

Brookes, C. P. & Loxdale, H. D. (1987). Survey of enzyme variation in British populations of Myzus persicae (Sulzer) (Hemiptera: Aphididae) on crops and weed hosts.—Bull. ent. Res. 77, 83–89.CrossRefGoogle Scholar
Devonshire, A. L., Foster, G. N. & Sawicki, R. M. (1977). Peach-potato aphid, Myzus persicae (Sulz.), resistant to organophosphorus and carbamate insecticides on potatoes in Scotland.—Pl. Path. 26, 60–62.CrossRefGoogle Scholar
Devonshire, A. L. & Moores, G. D. (1982). A carboxylesterase with broad substrate specificity causes organophosphorus, carbamate and pyrethroid resistance in peach-potato aphids (Myzus persicae).—Pestic. Biochem. & Physiol. 18, 235–246.CrossRefGoogle Scholar
Devonshire, A. L., Moores, G. D. & Ffrench-Constant, R. H. (1986). Detection of insecticide resistance by immunological estimation of carboxylesterase activity in Myzus persicae (Sulzer) and cross reaction of the antiserum with Phorodon humuli(Schrank) (Hemiptera: Aphididae).—Bull. ent. Res. 76, 97–107.CrossRefGoogle Scholar
Devonshire, A. L. & Sawicki, R. M. (1979). Insecticide-resistant Myzus persicae as an example of evolution by gene duplication.—Nature, Lond. 280, 140–141.CrossRefGoogle Scholar
Dunn, J. A. & Kempton, D. P. H. (1977). The development on potatoes of a glasshouse strain of Myzus persicae resistant to organophosphorus insecticides.—Ann. appl. Biol. 85, 175–179.Google Scholar
Ffrench-Constant, R. H. & Devonshire, A. L. (1986). The effect of aphid immigration on the rate of selection of insecticide resistance in Myzus persicae by different classes of insecticides.—pp. 115–125 in Aspects of Applied Biology 13, Crop protection of sugar beet and crop protection and quality of potatoes, 1986.—519 pp. Wellesbourne, AAB.Google Scholar
Ffrench-Constant, R. H. & Devonshire, A. L. (1987 a). A multiple homogenizer for rapid sample preparation in immunoassays and electrophoresis.—Biochem. Gen. 25, 493–499.CrossRefGoogle ScholarPubMed
Ffrench-Constant, R. H., Devonshire, A. L. & Clark, S. J. (1987 b). Differential rate of selection for resistance by carbamate, organophosphorus and combined pyrethroid and organophosphorus insecticides in Myzus persicae (Sulzer) (Hemiptera: Aphididae).—Bull. ent. Res. 77, 227–238.CrossRefGoogle Scholar
Ffrench-Constant, R. H., Devonshire, A. L. & White, R. P. (1988 a). Spontaneous loss and re-selection of resistance in extremely resistant Myzus persicae (Sulzer).—Pestic. Biochem. & Physiol. 29.Google Scholar
Ffrench-Constant, R. H., Harrington, R. & Devonshire, A. L. (1988 b). Effect of repeated application of insecticides to potatoes on numbers of Myzus persicae (Sulzer) (Hemiptera: Aphididae) and on the frequencies of insecticide-resistant variants.—Crop. Prot. 7, 55–61.Google Scholar
Furk, C. (1986). Incidence and distribution of insecticide-resistant strains of Myzus persicae (Sulzer) (Hemiptera: Aphididae) in England and Wales in 1980–84.—Bull. ent. Res. 76, 53–58.CrossRefGoogle Scholar
Rice, A. D., Devonshire, A. L., Gibson, R. W., Gooding, A. R., Moores, G. D. & Stribley, M. F. (1985). The problem of aphid resistance to aphicides, and alternative methods of preventing virus transmission.—pp. 209–228 in International Institute for Sugar Beet Research, 48th Winter Congress, Bruxelles, 1985.—445 pp. Belgium, J. Duculot.Google Scholar
Sawicki, R. M., Devonshire, A. L., Rice, A. D., Moores, G. D., Petzing, S. M. & Cameron, A. (1978). The detection and distribution of organophosphorus and carbamate insecticide- resistant Myzus persicae (Sulz.) in Britain in 1976.—Pestic. Sci. 9, 189–201.CrossRefGoogle Scholar
Sawicki, R. M., Rice, A. D. & Gibson, R. W. (1983). Insecticide-resistance in the peach-potato aphid Myzus persicae and the prevention of virus spread.—pp. 29–32 in Aspects of Applied Biology, 2. Pests, diseases, weeds and weed beet in sugar beet, 1983.—218 pp. Wellesbourne, AAB.Google Scholar
Sykes, G. B. (1977). Resistance in the peach-potato aphid (Myzus persicae (Sulz.)) to organophosphorus insecticides in Yorkshire and Lancashire.—Pl. Path. 26, 91–93.CrossRefGoogle Scholar