Hostname: page-component-84b7d79bbc-5lx2p Total loading time: 0 Render date: 2024-07-26T19:42:45.542Z Has data issue: false hasContentIssue false
  • English
  • Français

An integrated diagnostic setup for the morphological and molecular identification of the Ceratitis FAR complex (C. anonae, C. fasciventris, C. rosa, C. quilicii, Diptera, Tephritidae)

Published online by Cambridge University Press:  11 September 2018

M. Virgilio Open the ORCID record for M. Virgilio [Opens in a new window] ,
J.-H. Daneel ,
A. Manrakhan ,
H. Delatte Open the ORCID record for H. Delatte [Opens in a new window] ,
K. Meganck  and
M. De Meyer
M. Virgilio*
Affiliation:
Royal Museum for Central Africa, Leuvensesteenweg 13, B-3080 Tervuren, Belgium
J.-H. Daneel
Affiliation:
Citrus Research International, P.O Box 28, Nelspruit 1200, South Africa
A. Manrakhan
Affiliation:
Citrus Research International, P.O Box 28, Nelspruit 1200, South Africa
H. Delatte
Affiliation:
CIRAD-UMR PVBMT, CIRAD-3P, Saint Pierre, France
K. Meganck
Affiliation:
Royal Museum for Central Africa, Leuvensesteenweg 13, B-3080 Tervuren, Belgium
M. De Meyer
Affiliation:
Royal Museum for Central Africa, Leuvensesteenweg 13, B-3080 Tervuren, Belgium
*
*Author for correspondence Phone: +32 (0) 27695854 E-mail: massimiliano.virgilio@africamuseum.be
Get access Rights & Permissions [Opens in a new window]

Abstract

The Ceratitis FAR complex (Diptera, Tephritidae) includes four economically important frugivorous flies (Ceratitis anonae, Ceratitis fasciventris, Ceratitis quilicii, Ceratitis rosa) whose immature stages and adult females cannot be properly resolved through morphological identification. In order to develop a simplified molecular tool for the identification of two of these species (C. rosa, C. quilicii), we selected a subset of six microsatellite markers out of a panel of 16 loci that were previously developed for the molecular differentiation of the taxa within the complex. These six markers were first tested in silico and then used for the actual genotyping of C. quilicii and C. rosa, resulting in the correct identification of all male reference specimens. Here, we propose an integrated morphological and molecular setup for the identification of the four species of the FAR complex. The decision map relies on preliminary DNA barcoding or morphological identification (when possible) to exclude species not belonging to the complex followed by (a) morphological identification of all adult male specimens and female C. anonae, (b) molecular identification via a panel of 16 microsatellite markers for immature stages, damaged vouchers and samples potentially including adult female C. fasciventris/C. quilicii/C. rosa and (c) molecular identification via a reduced panel of six microsatellite markers for samples including only C. quilicii and C. rosa. This simplified diagnostic setup was profitably implemented in the framework of the ERAfrica fruit fly project and will help correctly identify species within the FAR complex for their early detection and monitoring.

Keywords

molecular identificationDNA barcodingmicrosatellitesC. quiliciiC. rosaFAR complexdecision map
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 109 , Issue 3 , June 2019 , pp. 376 - 382
Check for updates
Copyright
Copyright © Cambridge University Press 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Barr, N.B. & McPheron, B.A. (2006) Molecular phylogenetics of the genus Ceratitis (diptera: Tephritidae). Molecular Phylogenetics and Evolution 38, 216–230.Google Scholar
Barr, N.B. & Wiegmann, B.M. (2009) Phylogenetic relationships of Ceratitis fruit flies inferred from nuclear CAD and tango/ARNT gene fragments: testing monophyly of the subgenera Ceratitis (ceratitis) and C. (Pterandrus). Molecular Phylogenetics and Evolution 53, 412–424.Google Scholar
Barr, N.B., Islam, M.S., De Meyer, M. & McPheron, B.A. (2012) Molecular identification of Ceratitis capitata (Diptera: Tephritidae) using DNA sequences of the COI barcode region. Annals of the Entomological Society of America 105, 339–350.Google Scholar
Delatte, H., Virgilio, M., Simiand, C., Quilici, S. & Meyer, M. (2013) Isolation and characterisation of sixteen microsatellite markers cross-amplifying in a complex of three African agricultural pests (Ceratitis rosa, C. anonae and C. fasciventris, Diptera: Tephritidae). Conservation Genetics Resources 5, 31–34.Google Scholar
De Meyer, M. (1996) Revision of the subgenus Ceratitis (pardalaspis) Bezzi, 1918 (Diptera, Tephritidae, Ceratitini). Systematic Entomology 21, 15–26.Google Scholar
De Meyer, M. (1998) Revision of the subgenus Ceratitis (ceratalaspis) Hancock (Diptera: Tephritidae). Bulletin of Entomological Research 88, 257–290.Google Scholar
De Meyer, M. (2000) Systematic revision of the subgenus Ceratitis Macleay s.s. (Diptera, Tephritidae). Zoological Journal of the Linnean Society 128, 439–467.Google Scholar
De Meyer, M. & Freidberg, A. (2006) Revision of the subgenus Ceratitis (pterandrus) Bezzi (Diptera: Tephritidae). Israel Journal of Entomology 36, 197–315.Google Scholar
De Meyer, M., Clarke, A.R., Vera, T.M. & Hendrichs, J. (2015 a) Resolution of cryptic species complexes of tephritid pests to enhance SIT application and facilitate international trade. ZooKeys 540, 1–3.Google Scholar
De Meyer, M., Delatte, H., Ekesi, S., Jordaens, K., Kalinova, B., Manrakhan, A., Mwatawala, M., Steck, G., Van Cann, J., Vancikova, L., Brizova, R. & Virgilio, M. (2015 b) An integrative approach to unravel the Ceratitis FAR (diptera, Tephritidae) cryptic species complex: a review. ZooKeys 540, 405–427.Google Scholar
De Meyer, M., Mwatawala, M., Copeland, R.S. & Virgilio, M. (2016) Description of new Ceratitis species (Diptera: Tephritidae) from Africa, or how morphological and DNA data are complementary in discovering unknown species and matching sexes. European Journal of Taxonomy 233, 1–23.Google Scholar
De Villiers, M., Manrakhan, A., Addison, P. & Hattingh, V. (2013) The distribution, relative abundance, and seasonal phenology of Ceratitis capitata, Ceratitis rosa, and Ceratitis cosyra (Diptera: Tephritidae) in South Africa. Environmental Entomology 42, 831–840.Google Scholar
Dupuis, J.R., Bremer, F.T., Kauwe, A., Jose, M.S., Leblanc, L., Rubinoff, D. & Geib, S.M. (2017) HiMAP: robust phylogenomics from highly multiplexed amplicon sequencing. BioRxiv, 213454. doi: 10.1101/213454.Google Scholar
Drosopoulou, E., Pantelidou, C., Gariou-Papalexiou, A., Augustinos, A.A., Chartomatsidou, T., Kyritsis, G.A., Bourtzis, K., Mavragani-Tsipidou, P & Zacharopoulou, A. (2017) The chromosomes and the mitogenome of Ceratitis fasciventris (Diptera: Tephritidae): two genetic approaches towards the Ceratitis FAR species complex resolution. Scientific Reports 7, 4877.Google Scholar
Evanno, G., Regnaut, S. & Goudet, J. (2005) Detecting the number of clusters of individuals using the software structure: a simulation study. Molecular Ecology 14, 2611–2620.Google Scholar
Geurts, K., Mwatawala, M. & De Meyer, M. (2012) Indigenous and invasive fruit fly diversity along an altitudinal transect in Eastern Central Tanzania. Journal of Insect Science 12, 12.Google Scholar
Jombart, T. (2008) Adegenet: a R package for the multivariate analysis of genetic markers. Bioinformatics (Oxford, England) 24, 1403–1405.Google Scholar
Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S., Duran, C., Thierer, T., Ashton, B., Meintjes, P. & Drummond, A. (2012) Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics (Oxford, England) 28, 1647–1649.Google Scholar
Manrakhan, A., Daneel, J.H., Virgilio, M. & De Meyer, M. (2017) Sensitivity of an enriched ginger oil based trapping system for Ceratitis fruit fly pests (Diptera: Tephritidae). Crop Protection 99, 26–32.Google Scholar
Meier, R., Shiyang, K., Vaidya, G. & Ng, P.K.L. (2006) DNA barcoding and taxonomy in Diptera: a tale of high intraspecific variability and low identification success. Systematic Biology 55, 715–728.Google Scholar
Mwatawala, M., Virgilio, M., Joseph, J. & de Meyer, M. (2015) Niche partitioning among two Ceratitis rosa morphotypes and other Ceratitis pest species (Diptera, Tephritidae) along an altitudinal transect in Central Tanzania. ZooKeys 540, 429–442.Google Scholar
Pritchard, J.K., Stephens, M. & Donnelly, P. (2000) Inference of population structure using multilocus genotype data. Genetics 155, 945–959.Google Scholar
Steck, G.J. & Ekesi, S. (2015) Description of third instar larvae of Ceratitis fasciventris, C. anonae, C. rosa (FAR complex) and C. capitata (diptera, Tephritidae). ZooKeys 540, 443–466.Google Scholar
Tanga, C.M., Manrakhan, A., Daneel, J.-H., Mohamed, S.A., Fathiya, K. & Ekesi, S. (2015) Comparative analysis of development and survival of two Natal fruit fly Ceratitis rosa Karsch (Diptera, Tephritidae) populations from Kenya and South Africa. ZooKeys 540, 467–487.Google Scholar
Van Cann, J., Virgilio, M., Jordaens, K. & De Meyer, M. (2015) Wing morphometrics as a possible tool for the diagnosis of the Ceratitis fasciventris, C. anonae, C. rosa complex (Diptera, Tephritidae). ZooKeys 540, 489–506.Google Scholar
Vaníčková, L., Virgilio, M., Tomčala, A., Břízová, R., Ekesi, S., Hoskovec, M., Kalinová, B., Do Nascimento, R.R.R. & De Meyer, M. (2014) Resolution of three cryptic agricultural pests (Ceratitis fasciventris, C. anonae, C. rosa, Diptera: Tephritidae) using cuticular hydrocarbon profiling. Bulletin of Entomological Research 104, 631–638.Google Scholar
Virgilio, M., Backeljau, T., Barr, N. & De Meyer, M. (2008) Molecular evaluation of nominal species in the Ceratitis fasciventris, C. anonae, C. rosa complex (Diptera: Tephritidae). Molecular Phylogenetics and Evolution 48, 270–280.Google Scholar
Virgilio, M., Backeljau, T., Nevado, B. & De Meyer, M. (2010) Comparative performances of DNA barcoding across insect orders. BMC Bioinformatics 11, 206.Google Scholar
Virgilio, M., Jordaens, K., Breman, F.C., Backeljau, T. & De Meyer, M. (2012) Identifying insects with incomplete DNA barcode libraries, African fruit flies (Diptera: Tephritidae) as a test case. PLoS ONE 7, e31581.Google Scholar
Virgilio, M., Delatte, H., Quilici, S., Backeljau, T. & De Meyer, M. (2013) Cryptic diversity and gene flow among three African agricultural pests: Ceratitis rosa, Ceratitis fasciventris and Ceratitis anonae (Diptera, Tephritidae). Molecular Ecology 22, 2526–2539.Google Scholar
Virgilio, M., White, I.M. & De Meyer, M. (2014) A set of multi-entry identification keys to African frugivorous flies (Diptera, Tephritidae). ZooKeys 428, 97–108.Google Scholar
Supplementary material: File

Virgilio et al. supplementary material

Virgilio et al. supplementary material 1

Download Virgilio et al. supplementary material(File)
File 414.3 KB