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Clones of pea aphid, Acyrthosiphon pisum (Hemiptera: Aphididae) distinguished using genetic markers, differ in their damaging effect on a resistant alfalfa cultivar

Published online by Cambridge University Press:  02 March 2010

R. Bournoville ,
J.-C. Simon ,
I. Badenhausser ,
C. Girousse ,
T. Guilloux  and
S. André
R. Bournoville*
Affiliation:
Laboratoire de Zoologie, INRA, 86600 Lusignan, France
J.-C. Simon
Affiliation:
LIMR Biologie des Population et des Organismes appliquée à la Protection des Plantes, INRA, B.P. 29, 35650 Le Rheu, France
I. Badenhausser
Affiliation:
Laboratoire de Zoologie, INRA, 86600 Lusignan, France
C. Girousse
Affiliation:
Laboratoire de Zoologie, INRA, 86600 Lusignan, France
T. Guilloux*
Affiliation:
Laboratoire de Zoologie, INRA, 86600 Lusignan, France
S. André
Affiliation:
Laboratoire de Zoologie, INRA, 86600 Lusignan, France
*
*Fax: (33) 05 49 55 60 88 E-mail: bourno@lusignan.inra.fr
*Fax: (33) 05 49 55 60 88 E-mail: bourno@lusignan.inra.fr
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Abstract

CUF 101, a resistant cultivar of alfalfa, was exposed to 15 clones of Acyrthosiphon pisum Harris collected from alfalfa fields in three regions of France (east, south, central west) to determine whether the level of resistance varied across the different clones. The survival of alfalfa seedlings infested at the cotyledon stage was assessed using a standardized method. Although no difference in seedling mortality was detected between clones grouped by region, there was a significant variation among the 15 pea aphid clones. In particular, two clones of southern origin were more aggressive. In addition, the different pea aphid clones were characterized using allozyme and RAPD-PCR markers. Among the 15 clones, seven allozyme genotypes (plus one when adding colour polymorphism) and 12 RAPD-PCR genotypes were distinguished. The two southern clones differing by their aggressiveness on the resistant alfalfa belonged to the same allozyme and RAPD genotype which was distinct from the other pea aphid clones. Our results reinforce the need to take into account aphid genetic diversity in breeding programmes for resistance in cultivated plants.

Type
Review Article
Information
Bulletin of Entomological Research , Volume 90 , Issue 1 , February 2000 , pp. 33 - 39
Copyright
Copyright © Cambridge University Press 2000

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References

Berberet, R.C., Caddel, J.L. & Zarrabi, A.A. (1991) Pea aphid resistance. in Fox, C.C., Berberet, R., Gray, C.R., Grau, C.R., Jessen, D.L. & Peterson, M.A. (Eds) Standard tests to characterize alfalfa cultivars. 3rd edn. North American Alfalfa Improvement Conference.Google Scholar
Blackman, R.L. (1981) Aphid genetics and host plant resistance. Bulletin SROP 4, 13–19.Google Scholar
Bournoville, R. (1973) Observations écologiques sur l'hivernation du puceron du pois, Acyrthosiphon pisum (Harris) et ses parasites dans la région de Versailles. Annales de Zoologie et d'Ecologie animales 5, 13–28.Google Scholar
Bournoville, R. (1977) Etude de quelques relations entre le végétal (espèce, variété, stade phénologique) et le puceron du pois Acyrthosiphon pisum Harris (Homoptera, Aphididae). Annales de Zoologie et d’ Ecologie animales 9, 87–98.Google Scholar
Bournoville, R. (1980) Varietal characteristic under French conditions of some alfalfa cultivars selected for resistance to two insects. EPPO Bulletin 10, 317–322.CrossRefGoogle Scholar
Bournoville, R. (1981) Variability of the net reproductive rate of clones of the pea aphid on alfalfa. Bulletin 4, 129–132.Google Scholar
Bournoville, R., Landré, B., Aupinel, P., Girousse, C. & Badenhausser, I. (1999) Test en conditions contrôlées de la résistance de la luzerne au puceron du pois. Fourrages 158, 157–168.Google Scholar
Caillaud, C.M., Dedryver, C.A., Di Pietro, J.P., Simon, J.C., Fima, F. & Chaubet, B. (1995) Clonal variability in the response of Sitobion avenae (Homoptera: Aphididae) to resistant and susceptible wheat. Bulletin of Entomological Research 85, 189–195.CrossRefGoogle Scholar
Claridge, M.F. & Den Hollander, J. (1983) The biotype concept and its application to insect pests of agriculture. Crop Protection 2, 85–95.CrossRefGoogle Scholar
Dalmasso, A. & Bournoville, R. (1983) Isoenzymes d'une population d'Acyrthosiphon pisum Harris (Hom.Aphididae). Agronomie 9, 891–896.CrossRefGoogle Scholar
De Barro, P.J., Sherratt, T.N., Brookes, C.P., David, O. & Maclean, N. (1995) Spatial and temporal genetic variation in British field populations of the grain aphid Sitobion avenae (F.) (Hemiptera: Aphididae) studied using RAPD–PCR. Proceedings of the Royal Society of London, Series B 262, 321–327.Google ScholarPubMed
De Barro, P.J., Sherratt, T.N., David, O. & Maclean, N. (1995) An investigation of the differential performance of clones of the aphid Sitobion avenae on two host species. Oecologia 104, 379–385.CrossRefGoogle ScholarPubMed
Dixon, A.F.G. (1998) Aphid ecology. 2nd edn, 300 pp. London, Chapman & Hall.Google Scholar
Girousse, C., Bournoville, R. & Badenhausser, I. (1999) Evaluation of alfalfa resistance to the pea aphid, Acyrthosiphon pisum (Homoptera, Aphididae). Methodological aspects to improve a standardized seedling test. Phytoprotection 79, 139–148.CrossRefGoogle Scholar
Guirao, P., Beitia, F. & Cenis, J.L. (1997) Biotype determination of Spanish populations of Bemisia tabaci (Hemiptera: Aleyrodidae). Bulletin of Entomological Research 87, 587–593.CrossRefGoogle Scholar
Hales, D.H., Tomiuk, J., Wöhrmann, K. & Sunnucks, P. (1997) Evolutionary and genetic aspects of aphid biology: a review. European Journal of Entomology 94, 1–55.Google Scholar
Hebert, P.D.N. & Beaton, M.J. (1989) Methodologies for allozyme analysis using cellulose acetate electrophoresis. Helena Laboratories, Beaumont, Texas.Google Scholar
Hille Ris Lambers, D. (1947) Contribution to a monograph of the Aphididae of Europe. Temminckia 7, 179–319.Google Scholar
Lehman, W.F., Nielson, M.W., Marble, V.L. & Stanford, E.H. (1983) Registration of CUF 101 alfalfa. Crop Science 23, 398.CrossRefGoogle Scholar
Loxdale, H.D. & Lushai, G. (1998) Molecular markers in entomology. Bulletin of Entomological Research 88, 577–600.CrossRefGoogle Scholar
Mackay, P.A. & Lamb, R.J. (1988) Genetic variation in asexual populations of two aphids in the genus Acyrthosiphon, from an Australian alfalfa field. Entomologia Experimentalis et Applicata 48, 117–125.CrossRefGoogle Scholar
Martinez–Torres, D., Carrio, R., Latorre, A., Simon, J.–C., Hermoso, A. & Moya, A. (1997) Assessing the nucleotide diversity of three aphid species by RAPD. Journal of Evolutionary Biology 10, 459–477.CrossRefGoogle Scholar
Müller, F.P. (1980) Wirtspflanzen, generationenfolge und reproduktive isolation infraspezifischer formen von Acyrthosiphon pisum. Entomologia Experimentalis et Applicata 28, 145–157.CrossRefGoogle Scholar
Puterka, G.J. & Burton, R.L. (1990) Aphids genetics in relation to host plant resistance. pp. 59–69 in Peters, D.C., Webster, J.A. & Chlouber, C.S. (Eds) Aphid–plant interactions: population to molecule. Proceedings International Symposium Stillwater, Oklahoma, USA.Google Scholar
Puterka, G.J. & Peters, D.C. (1989) Inheritance of greenbug virulence to Gb2 and Gb3 resistance genes in wheat. Genome 32, 109–114.CrossRefGoogle Scholar
Sandström, J. (1994) High variation in host adaptation among clones of the pea aphid, Acyrthosiphon pisum on peas, Pisum sativum. Entomologia Experimentalis et Applicata 71, 245–256.CrossRefGoogle Scholar
Scheffé, H. (1953) A method for judging all contrasts in the analysis of variance. Biometrika 40, 87–104.Google Scholar
Simon, J.–C., Martinez–Torres, D., Latorre, A., Moya, A. & Hebert, P.D.N. (1996) Molecular characterization of cyclic and obligate parthenogens in the aphid Rhopalosiphum padi L. Proceedings of the Royal Society of London 263, 481–486.Google ScholarPubMed
Simon, J.–C., Baumann, S., Sunnucks, P., Hebert, P.D.N., Pierre, J.–S., Le Gallic, J.–F. & Dedryver, C.–A. (1999) Reproductive mode and population genetic structure of the cereal aphid Sitobion avenae studied using phenotypic and microsatellite markers. Molecular Ecology 8, 531–545.CrossRefGoogle ScholarPubMed
Simon, J.P., Parent, M.A. & Auclair, J.L. (1982) Isozyme analysis of biotypes and field populations of the pea aphid, Acyrthosiphon pisum. Entomologia Experimentalis et Applicata 32, 186–192.CrossRefGoogle Scholar
Sorensen, E.L., Byers, R.A. & Horber, E.K. (1988) Breeding for insect resistance. pp. 859–902 in Hanson, A.A., Barnes, D.K. & Hill, R.R. (Eds) Alfalfa and alfalfa improvement. American Society of Agronomy 29.Google Scholar
Sunnucks, P., England, P.R., Taylor, A.C. & Hales, D.F. (1996) Microsatellite and chromosome evolution of parthenogenetic Sitobion aphids in Australia. Genetics 144, 747–756.CrossRefGoogle ScholarPubMed
Sunnucks, P., Driver, F., Brown, W.V., Carver, M., Hales, D.F. & Milne, W.M. (1997) Biological and genetic characterization of morphologically similar Therioaphis trifolii (Hemiptera: Aphididae) with different host utilization. Bulletin of Entomological Research 87, 425–436.CrossRefGoogle Scholar
Suomalainen, E., Saura, A., Lokki, J. & Teeri, T. (1980) Genetic polymorphism and evolution in parthenogenetic animals. Theoretical and Applied Genetics 57, 129–132.CrossRefGoogle ScholarPubMed
Vanlerberghe–Masutti, F. & Chavigny, P. (1998) Host–based genetic differentiation in the aphid Aphis gossypii Glover, evidenced from RAPD fingerprints. Molecular Ecology 7, 905–914.CrossRefGoogle Scholar
Via, S. (1989) Field estimation of variation in host plant use between local populations of pea aphids from two crops. Ecological Entomology 14, 357–364.CrossRefGoogle Scholar
Via, S. (1991) The genetic structure of host plant adaptation in a spatial patchwork: demographic variability among reciprocally transplanted pea aphid clones. Evolution 45, 827–852.CrossRefGoogle Scholar