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Effect of Wolbachia on insecticide susceptibility in lines of Aedes aegypti

Published online by Cambridge University Press:  13 November 2012

N.M. Endersby  and
A.A. Hoffmann
N.M. Endersby*
Affiliation:
Department of Genetics, Bio21 Institute, The University of Melbourne, Victoria 3010, Australia
A.A. Hoffmann
Affiliation:
Department of Genetics, Bio21 Institute, The University of Melbourne, Victoria 3010, Australia
*
* Author for correspondence Fax: +61 3 8344 2279 E-mail: nancye@unimelb.edu.au
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Abstract

Two stable infections of Wolbachia pipientis, wMelPop and wMel, now established in Aedes aegypti, are being used in a biocontrol program to suppress the transmission of dengue. Any effects of Wolbachia infection on insecticide resistance of mosquitoes may undermine the success of this program. Bioassays of Ae. aegypti were conducted to test for differences in response to insecticides between Wolbachia infected (wMelPop, wMel) and uninfected lines. Insecticides screened were bifenthrin, the pyrethroid commonly used for adult knockdown, as well as larvicides: Bacillus thuringiensis var. israelensis, the organophosphate, temephos and the insect growth regulator, s-methoprene. While differences in response between lines were detected for some insecticides, no obvious or consistent effects related to presence of Wolbachia infection were observed. Spreading Wolbachia infections are, therefore, unlikely to affect the efficacy of traditional chemical control of mosquito outbreaks.

Keywords

Aedes aegyptWolbachiainsecticide resistancedengue
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 103 , Issue 3 , June 2013 , pp. 269 - 277
Copyright
Copyright © Cambridge University Press 2012

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References

Berticat, C., Rousset, F., Raymond, M., Berthomieu, A. & Weill, M. (2002) High Wolbachia density in insecticide–resistant mosquitoes. Proceedings of the Royal Society of London, Series B: Biological Sciences 269, 1413–1416.Google Scholar
Duron, O., Labbé, P., Berticat, C., Rousset, F., Guillot, S., Raymond, M. & Weill, M. (2006) High Wolbachia density correlates with cost of infection for insecticide resistant Culex pipiens mosquitoes. Evolution 60, 303–314.Google Scholar
Evans, O., Caragata, E.P., McMeniman, C.J., Woolfit, M., Green, D.C., Williams, C.R., Franklin, C.E., O'Neill, S.L. & McGraw, E.A. (2009) Increased locomotor activity and metabolism of Aedes aegypti infected with a life-shortening strain of Wolbachia pipientis . Journal of Experimental Biology 212, 1436–1441.Google Scholar
Ghanim, M. & Kontsedalov, S. (2009) Susceptibility to insecticides in the Q biotype of Bemisia tabaci is correlated with bacterial symbiont densities. Pest Management Science 65, 939–942.Google Scholar
Harburguer, L., Seccacini, E., Licastro, S., Zerba, E. & Masuh, H. (2012) Droplet size and efficacy of an adulticide-larvicide ultralow-volume formulation on Aedes aegypti using different solvents and spray application methods. Pest Management Science 68, 137–141.Google Scholar
Hoffmann, A.A., Montgomery, B.L., Popovici, J., Iturbe-Ormaetxe, I., Johnson, P.H., Muzzi, F., Greenfield, M., Durkan, M., Leong, Y.S., Dong, Y., Cook, H., Axford, J., Callahan, A.G., Kenny, N., Omodei, C., McGraw, E.A., Ryan, P.A., Ritchie, S. A., Turelli, M. & O'Neill, S.L. (2011) Successful establishment of Wolbachia in Aedes populations to suppress dengue transmission. Nature 476, 454–457.Google Scholar
Lee, S.F., White, V.L., Weeks, A.R., Hoffmann, A.A. & Endersby, N.M. (2012) High-throughput PCR assays to monitor Wolbachia infection in the dengue mosquito (Aedes aegypti) and Drosophila simulans . Applied and Environmental Microbiology 78, 4740–4743.Google Scholar
Oki, M., Sunahara, T., Hashizume, M. & Yamamoto, T. (2011) Optimal timing of insecticide fogging to minimize dengue cases: modeling dengue transmission among various seasonalities and transmission intensities. Plos Neglected Tropical Diseases 5(10), e1367.Google Scholar
Panteleev, D., Goryacheva, I., Andrianov, B., Reznik, N., Lazebny, O. & Kulikov, A. (2007) The endosymbiotic bacterium Wolbachia enhances the nonspecific resistance to insect pathogens and alters behavior of Drosophila melanogaster . Russian Journal of Genetics 43, 1066–1069.Google Scholar
Paz-Soldan, V.A., Plasai, V., Morrison, A.C., Rios-Lopez, E.J., Guedez-Gonzales, S., Grieco, J.P., Mundal, K., Chareonviriyaphap, T. & Achee, N.L. (2011) Initial assessment of the acceptability of a push-pull Aedes aegypti control strategy in Iquitos, Peru and Kanchanaburi, Thailand. American Journal of Tropical Medicine and Hygiene 84, 208–217.Google Scholar
Ranson, H., Burhani, J., Lumjuan, N. & Black, W.C. IV (2010) Insecticide resistance in dengue vectors. TropIKA.net 1, 0–0.Google Scholar
Rodriguez, M.M., Bisset, J.A. & Fernandez, D. (2007) Levels of insecticide resistance and resistance mechanisms in Aedes aegypti from some Latin American countries. Journal of the American Mosquito Control Association 23, 420–429.Google Scholar
Teixeira, L., Ferreira, A. & Ashburner, M. (2008) The bacterial symbiont Wolbachia induces resistance to RNA viral infections in Drosophila melanogaster . Plos Biology 6(12), e1000002.Google Scholar
Turley, A.P., Moreira, L.A., O'Neill, S.L. & McGraw, E.A. (2009) Wolbachia infection reduces blood-feeding success in the dengue fever mosquito, Aedes aegypti . Plos Neglected Tropical Diseases 3, e516.Google Scholar
Walker, T., Johnson, P.H., Moreira, L.A., Iturbe-Ormaetxe, I., Frentiu, F.D., McMeniman, C.J., Leong, Y.S., Dong, Y., Axford, J., Kriesner, P., Lloyd, A.L., Ritchie, S.A., O'Neill, S.L. & Hoffmann, A.A. (2011) The wMel Wolbachia strain blocks dengue and invades caged Aedes aegypti populations. Nature 476, 450–453.CrossRefGoogle ScholarPubMed
Wong, Z.S., Hedges, L.M., Brownlie, J.C. & Johnson, K.N. (2011) Wolbachia-mediated antibacterial protection and immune gene regulation in Drosophila . Plos One 6(9), e25430.CrossRefGoogle ScholarPubMed
Yeap, H.L., Mee, P., Walker, T., Weeks, A.R., O'Neill, S.L., Johnson, P., Ritchie, S.A., Richardson, K.M., Doig, C., Endersby, N.M. & Hoffmann, A.A. (2011) Dynamics of the “popcorn” Wolbachia infection in outbred Aedes aegypti informs prospects for mosquito vector control. Genetics 187, 583–595.Google Scholar