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Genetic relationships among biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae) based on AFLP analysis

Published online by Cambridge University Press:  09 March 2007

M.T. Cervera ,
J.A. Cabezas ,
B. Simón ,
J.M. Martínez-Zapater ,
F. Beitia  and
J.L. Cenis
M.T. Cervera
Affiliation:
CNB-CSIC, Campus de Cantoblanco, 28049 Madrid, Spain SGIT-INIA, Ctra. de La Coruña, Km 7.5, 28040 Madrid, Spain
J.A. Cabezas
Affiliation:
CNB-CSIC, Campus de Cantoblanco, 28049 Madrid, Spain SGIT-INIA, Ctra. de La Coruña, Km 7.5, 28040 Madrid, Spain
B. Simón
Affiliation:
CIDA, c/Mayor s/n, 30150 La Alberca (Murcia), Spain
J.M. Martínez-Zapater
Affiliation:
CNB-CSIC, Campus de Cantoblanco, 28049 Madrid, Spain SGIT-INIA, Ctra. de La Coruña, Km 7.5, 28040 Madrid, Spain
F. Beitia
Affiliation:
SGIT-INIA, Ctra. de La Coruña, Km 7.5, 28040 Madrid, Spain
J.L. Cenis*
Affiliation:
CIDA, c/Mayor s/n, 30150 La Alberca (Murcia), Spain
*
*Fax: +34 968 366 792 E-mail: JoseL.Cenis@carm.es
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Abstract

Genetic similarities between 13 samples belonging to nine reference biotypes and two field populations of Bemisia tabaci (Gennadius), one field population of B. medinae Gómez-Menor and another of B. afer Priesner &Hosny, were evaluated using amplified fragment length polymorphism (AFLP) markers. The results indicate that B. tabaci biotypes can be grouped together with a minimum similarity coefficient of 0.32 and separated from the two other species with a similarity coefficient of 0.07. Bemisia tabaci biotypes were grouped in four clusters which comprised: (i) Near East and Indian subcontinent biotypes; (ii) B and Q biotypes plus a Nigerian population from cowpea; (iii) New World A biotype; and (iv) S biotype and a Nigerian population from cassava. These results were consistent with a previous grouping of biotypes based on RAPD-PCR analysis. The AFLP assay allowed the scoring of a total of 354 polymorphic bands in two reaction events with the use of two primer combinations.

Type
Research Article
Information
Bulletin of Entomological Research , Volume 90 , Issue 5 , October 2000 , pp. 391 - 396
Copyright
Copyright © Cambridge University Press 2000

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References

Abdullahi, I., Atiri, G., Winter, S. & Thottappilly, G. (1998) Differentiation of whitefly (Bemisia tabaci) biotypes from different regions in Africa and their relation to the occurrence of cassava mosaic geminiviruses (in German). Mitteilungen an die Biologische Bundesanstalt Braunschweig 357, 284284.Google Scholar
Banks, G.K., Bedford, I.D., Beitia, F.J., Rodríguez-Cerezo, E. & Markham, P.G. (1999) A novel geminivirus of Ipomoea indica (Convolvulaceae) from Southern Spain. Plant Disease 83, 486.CrossRefGoogle ScholarPubMed
Bedford, I.D., Briddon, R.W., Markham, P.G., Brown, J.K. & Rosell, R.C. (1992) Bemisia tabaci: biotype characterization and the threat of this whitefly species to agriculture. Proceedings 1992 British Crop Protection Conference – Pests and Diseases 3, 1235–1240.Google Scholar
Bedford, I.D., Briddon, R.W., Markham, P.G., Brown, J.K. & Rosell, R.C. (1993) A new species of Bemisia or biotype of Bemisia tabaci (Genn.) as a future pest of European agriculture. Plant Health and the European Single Market, BCPC Monograph 54, 381–386.Google Scholar
Bedford, I.D., Briddon, R.W., Brown, J.K., Rosell, R.C. & Markham, P.G. (1994) Geminivirus transmission and biological characterisation of Bemisia tabaci (Gennadius) biotypes from different geographic regions. Annals of Applied Biology 125, 311–325.CrossRefGoogle Scholar
Bethke, J.A., Paine, T.D. & Nuessly, G.S. (1991) Comparative biology, morphometrics, and development of two populations of Bemisia tabaci (Homoptera: Aleyrodidae) on cotton and poinsettia. Annals of the Entomological Society of America 84, 407–411.CrossRefGoogle Scholar
Bird, J. (1957) A whitefly-transmitted mosaic of Jatropha gossypifolia. Agricultural Experimental Station University of Puerto Rico 22, 1–35.Google Scholar
Brown, J.K., Coats, S.A., Bedford, I.D., Markham, P.G., Bird, J. & Frohlich, D.R. (1995a) Characterization and distribution of esterase electromorphs in the whitefly, Bemisia tabaci (Genn.) (Homoptera: Aleyrodidae). Biochemical Genetics 33, 205–214CrossRefGoogle ScholarPubMed
Brown, J.K., Frohlich, D.R. & Rossell, R.C. (1995b) The sweetpotato or silverleaf whiteflies: biotypes of Bemisia tabaci or a species complex? Annual Review of Entomology 40, 511–534.CrossRefGoogle Scholar
Brown, J.K., Bird, J., Frohlich, D.R., Rosell, R.C., Bedford, I.D. & Markham, P.D. (1996) The relevance of variability within the Bemisia tabaci species complex to epidemics caused by subgroup III geminiviruses. pp. 77–89in Gerling, D. & Mayer, R.T.(Eds) Bemisia: 1995. Taxonomy, biology, damage, control and management. Andover, UK, Intercept.Google Scholar
Burban, C., Fishpool, L.D.C., Fauquet, C., Fargette, D. & Thouvenel, J.C. (1992) Host associated biotypes within West African populations of the whitefly Bemisia tabaci (Genn.), (Hom., Aleyrodidae). Journal of Applied Entomology 113, 416–423.CrossRefGoogle Scholar
Byrne, F.J., Bedford, I.D., Devonshire, A.L. & Markham, P.G. (1995) Esterase variation and squash induction in B-type Bemisia tabaci (Homoptera: Aleyrodidae). Bulletin of Entomological Research 85, 175–179.CrossRefGoogle Scholar
Cenis, J.L., Perez, P. & Fereres, A. (1993) Identification of aphid (Homoptera:Aphididae) species and clones by random amplified polymorphic DNA. Annals of the Entomological Society of America 86, 545–550.CrossRefGoogle Scholar
Cervera, M.T., Gusmao, J., Steenackers, M., Peleman, J., Storme, V., van den Broeck, A., van Montagu, M. & Boerjan, W. (1996) Identification of AFLP molecular markers for resistance against Melampsora larici-populina in Populus. Theoretical and Applied Genetics 93, 733–737.CrossRefGoogle ScholarPubMed
Costa, H.S. & Brown, J.K. (1991) Variation in biological characteristics and esterase patterns among populations of Bemisia tabaci Genn. and the association of one population with silverleaf symptom induction. Entomologia Experimentalis et Applicata 61, 211–219.CrossRefGoogle Scholar
Costa, H.S., Brown, J.K., Sivasupramaniam, S. & Bird, J. (1993) Regional distribution, insecticide resistance and reciprocal crosses between the A and B biotypes of Bemisia tabaci. Insecticide Science and its Application 14, 255–266.Google Scholar
Frohlich, D.R., Torres-Jerez, I., Bedford, I.D., Markham, P.G. & Brown, J.K. (1999) A phylogeographical analysis of the Bemisia tabaci species complex based on mitochondrial DNA markers. Molecular Ecology 8, 1683–1691.CrossRefGoogle ScholarPubMed
Gawel, N.J. & Bartlett, A.C. (1993) Characterization of differences between whiteflies using RAPD-PCR. Insect Molecular Biology 2, 33–38.CrossRefGoogle ScholarPubMed
Gómez-Menor, J. (1954) Aleuródidos de España, Islas Canarias y Africa Occidental. 3a nota. Eos 30, 363–377.Google Scholar
Guirao, P., Beitia, F. & Cenis, J.L. (1997) Biotype determination in Spanish populations of Bemisia tabaci (Hemiptera: Aleyrodidae). Bulletin of Entomological Research 87, 587–593.CrossRefGoogle Scholar
Mantel, N. (1967) The detection of disease clustering and a generalized regression approach. Cancer Research 27, 209–220.Google Scholar
Perring, T.M., Cooper, A.D., Rodríguez, R.J., Farrar, C.A. & Bellows, T.S.J. (1993) Identification of a whitefly species by genomic and behavioral studies. Science 259, 74–77.CrossRefGoogle ScholarPubMed
Reineke, A., Karlovsky, P. & Zebitz, C.P.W. (1998) Preparation and purification of DNA from insects for AFLP analysis. Insect Molecular Biology 7, 95–99.CrossRefGoogle ScholarPubMed
Reineke, A., Karlovsky, P. & Zebitz, C.P.W. (1999) Amplified fragment length polymorphism analysis of different geographic populations of the gypsy moth, Lymantria dispar (Lepidoptera: Lymantridae). Bulletin of Entomological Research 89, 79–88.CrossRefGoogle Scholar
Rohlf, F.J. (1993) NTSYS-PC numerical taxonomy and multivariate analysis system. Version 1.8, Setauket, Nueva York, Exeter.Google Scholar
Sneath, P.H.A. & Sokal, R.R. (1973) Numerical taxonomy. San Francisco, California, Freeman and Company.Google Scholar
Vos, P., Hogers, R., Bleeker, M., Reijans, M., van de Lee, T., Hornes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M. & Zabeau, M. (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23, 4407–4414.CrossRefGoogle ScholarPubMed
Williams, J.G.K., Kubelik, A.R., Livack, K.J., Rafalsky, J.A. & Tingey, S.V. (1990) DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18, 6531–6535.CrossRefGoogle ScholarPubMed
Wool, D., Gerling, D., Belloti, A.C. & Morales, F.J. (1993) Esterase electrophoretic variation in Bemisia tabaci (Genn.) (Hom., Aleyrodidae) among host plants and localities in Israel. Journal of Applied Entomology 115, 185–196.CrossRefGoogle Scholar