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SlCarE054 in Spodoptera litura (Lepidoptera: Noctuidae) showed direct metabolic activity to β-cypermethrin with stereoselectivity

Published online by Cambridge University Press:  06 May 2024

Li Xu ,
Hongyu Liu ,
Bo Li ,
Guangling Li ,
Runqiang Liu Open the ORCID record for Runqiang Liu [Opens in a new window]  and
Dongzhi Li Open the ORCID record for Dongzhi Li [Opens in a new window]
Li Xu
Affiliation:
Henan Engineering Research Center of Green Pesticide Creation & Intelligent Pesticide Residue Sensor Detection, Henan Institute of Science and Technology, Xinxiang 453003, China College of Resources and Environment, Henan Institute of Science and Technology, Xinxiang 453003, China
Hongyu Liu
Affiliation:
Henan Engineering Research Center of Green Pesticide Creation & Intelligent Pesticide Residue Sensor Detection, Henan Institute of Science and Technology, Xinxiang 453003, China College of Resources and Environment, Henan Institute of Science and Technology, Xinxiang 453003, China
Bo Li
Affiliation:
Henan Engineering Research Center of Green Pesticide Creation & Intelligent Pesticide Residue Sensor Detection, Henan Institute of Science and Technology, Xinxiang 453003, China College of Resources and Environment, Henan Institute of Science and Technology, Xinxiang 453003, China
Guangling Li
Affiliation:
Henan Engineering Research Center of Green Pesticide Creation & Intelligent Pesticide Residue Sensor Detection, Henan Institute of Science and Technology, Xinxiang 453003, China College of Resources and Environment, Henan Institute of Science and Technology, Xinxiang 453003, China
Runqiang Liu*
Affiliation:
Henan Engineering Research Center of Green Pesticide Creation & Intelligent Pesticide Residue Sensor Detection, Henan Institute of Science and Technology, Xinxiang 453003, China College of Resources and Environment, Henan Institute of Science and Technology, Xinxiang 453003, China
Dongzhi Li
Affiliation:
Henan Engineering Research Center of Green Pesticide Creation & Intelligent Pesticide Residue Sensor Detection, Henan Institute of Science and Technology, Xinxiang 453003, China College of Resources and Environment, Henan Institute of Science and Technology, Xinxiang 453003, China
*
Corresponding authors: Runqiang Liu; Email: liurqhist@126.com; Dongzhi Li; Email: lidongzhi1989@163.com
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Abstract

Carboxylesterases (CarEs) is an important detoxification enzyme system in phase Ⅰ participating in insecticides resistance. In our previous study, SlCarE054, a CarEs gene from lepidoptera class, was screened out to be upregulated in a pyrethroids and organophosphates resistant population. Its overexpression was verified in two field-collected populations of Spodoptera litura (Lepidoptera: Noctuidae) resistant to pyrethroids and organophosphates by qRT-PCR. Spatiotemporal expression results showed that SlCarE054 was highly expressed in the pupae stage and the digestive tissue midgut. To further explore its role in pyrethroids and organophosphates resistance, its metabolism activity to insecticides was determined by UPLC. Its recombinant protein showed significant metabolism activity to cyhalothrin and fenvalerate, but not to phoxim or chlorpyrifos. The metabolic activity of SlCarE054 to β-cypermethrin showed stereoselectivity, with higher metabolic activity to θ-cypermethrin than the enantiomer α-cypermethrin. The metabolite of β-cypermethrin was identified as 3-phenoxybenzaldehyde. Further modelling and docking analysis indicated that β-cypermethrin, cyhalothrin and fenvalerate could bind with the catalytic triad of the 3D structure of SlCarE054. The interaction of β-cypermethrin with SlCarE054 also showed the lowest binding energy. Our work provides evidence that SlCarE054 play roles in β-cypermethrin resistance in S. litura.

Keywords

heterologous expressionmetabolic activitySlCarE054stereoselectivityβ-cypermethrin
Type
Research Paper
Information
Bulletin of Entomological Research , First View , pp. 1 - 9
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Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press

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Footnotes

*

These authors contributed equally to this work.

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