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Comparative activity of three isolates of LdMNPV against two strains of Lymantria dispar

Published online by Cambridge University Press:  02 April 2012

Peter M. Ebling ,
Imre S. Otvos  and
Nicholas Conder
Peter M. Ebling*
Affiliation:
Natural Resources Canada, Canadian Forest Service, Great Lakes Forestry Centre, 1219 Queen Street East, Sault Ste. Marie, Ontario, Canada P6A 2E5
Imre S. Otvos
Affiliation:
Natural Resources Canada, Canadian Forest Service, Pacific Forestry Centre, 506 West Burnside Road, Victoria, British Columbia, Canada V8Z 1M5
Nicholas Conder
Affiliation:
Natural Resources Canada, Canadian Forest Service, Pacific Forestry Centre, 506 West Burnside Road, Victoria, British Columbia, Canada V8Z 1M5
*
1Corresponding author (e-mail: pebling@nrcan.gc.ca).
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Abstract

Two newly identified geographic isolates of a nucleopolyhedrovirus (LdMNPV-H and LdMNPV-J) were evaluated against Disparvirus (LdMNPV-D), a baculovirus insecticide registered in Canada for the control of gypsy moth (Lymantria dispar (L.) (Lepidoptera: Lymantriidae)). Profiles of HindIII and EcoRI restriction enzyme digests of viral DNA from the three viral isolates are presented. Viral isolates were bioassayed using an inoculated diet plug method to determine the dose- and time-responses of second-instar larvae of both the European and Asian strains of gypsy moth. LdMNPV-D was found to be the most virulent isolate when tested against the European strain of gypsy moth, yielding an LD50 and an LD95 (estimates of the doses required to kill 50% and 95% of the test larvae, respectively) of 95 and 774 occlusion bodies (OBs), respectively. LdMNPV-H was the most virulent isolate when tested against the Asian strain, yielding an LD50 and an LD95 of 648 and 8540 OBs, respectively. Time-response data indicate that the three isolates differ little with respect to their speed of kill (ST50; estimate of the length of time required to kill 50% of the test insects) of either larval strain. These results indicate that both new isolates (LdMNPV-H and LdMNPV-J) should be investigated further for control of the Asian strain of the gypsy moth but not pursued for control of the European strain.

Résumé

Nous évaluons deux souches géographiques récemment identifiées du virus de la polyédrose nucléaire (LdMNPV-H et LdMNPV-J) par comparaison au Disparvirus (LdMNPV-D), un insecticide à base de baculovirus homologué au Canada pour la lutte contre la spongieuse (Lymantria dispar (L.) (Lepidoptera: Lymantriidae)). Nous présentons les profils des produits de digestion de l'ADN viral des trois souches de virus par les enzymes de restriction HindIII et EcoRI. Nous avons fait des bioessais avec les souches virales au moyen de la méthode d'inoculation de pastilles alimentaires pour déterminer les réactions en fonction du temps et de la dose des larves de second stade des races européenne et asiatique de la spongieuse. LdMNPV-D est la souche la plus virulente contre la race européenne de la spongieuse, avec des LD50 et LD95 (estimation des doses respectives nécessaires pour tuer 50 % et 95 % des larves testées) respectivement de 95 et 774 OB (corps d'occlusion). LdMNVP est la souche la plus virulente contre la race asiatique de spongieuse, avec des LD50 et LD95 respectivement de 648 et de 8540 OB. Les réactions en fonction du temps indiquent que les trois souches diffèrent très peu dans le temps requis pour tuer (ST50, estimation du temps nécessaire pour tuer 50 % des larves testées) l'une ou l'autre des races de larves. Ces résultats indiquent que les deux souches nouvelles (LdMNPV-H et LdMNPV-J) devraient être étudiées davantage en vue de la lutte contre la race asiatique de la spongieuse, mais non contre la race européenne.

[Traduit par la Rédaction]

Type
Articles
Information
The Canadian Entomologist , Volume 136 , Issue 5 , October 2004 , pp. 737 - 747
Copyright
Copyright © Entomological Society of Canada 2004

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