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HOECHST STAINING AND QUANTIFICATION OF SPERM IN THE SPERMATOPHORE AND SPERMATHECA OF THE DECORATED CRICKET, GRYLLODES SUPPLICANS (ORTHOPTERA: GRYLLIDAE)

Published online by Cambridge University Press:  31 May 2012

Scott K. Sakaluk  and
Danton H. O'Day
Scott K. Sakaluk
Affiliation:
Department of Zoology, Erindale College, University of Toronto, Mississauga, Ontario L5L 1C6
Danton H. O'Day
Affiliation:
Department of Zoology, Erindale College, University of Toronto, Mississauga, Ontario L5L 1C6
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Abstract

The use of Hoechst (33258) stain in quantifying spermatozoa contained in the spermatheca and spermatophore of the decorated cricket, Gryllodes supplicans (Walker) (Orthoptera: Gryllidae), is described. The technique was used to estimate the number of sperm remaining in spermatophores removed from females at various times after females had mated.

Résumé

On décrit l'utilisation du colorant de Hoechst (33258) pour quantifier les spermatozoïdes contenus dans la spermathèque et le spermatophore du grillon Gryllodes supplicans (Walker) (Orthoptera : Gryllidae). Cette technique a été utilisée pour estimer le nombre de spermatozoïdes demeurant dans des spermatophores retirés de femelles à des temps variables après l'accouplement.

Type
Articles
Information
The Canadian Entomologist , Volume 116 , Issue 12 , December 1984 , pp. 1585 - 1589
Copyright
Copyright © Entomological Society of Canada 1984

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