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A METHOD FOR SEPARATING AND SURFACE STERILIZING THE EGGS OF THE SPRUCE BUDWORM, CHORISTONEURA FUMIFERANA (LEPIDOPTERA: TORTRICIDAE)1
Published online by Cambridge University Press: 31 May 2012
Abstract
A simple method for separating and surface sterilizing spruce budworm eggs is described. The egg masses are incubated with constant agitation in a 1% solution of NaOH for 10 minutes at 35 °C. The separated eggs are rinsed serially in distilled water, 1% bovine albumen solution, and 70% ethanol, and placed on a moist, sterile filter paper in a petri dish. When surface sterilized eggs are not required the 70% ethanol wash can be omitted.
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- Copyright © Entomological Society of Canada 1971
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