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Note on Specific Gravity of Haemolymph of Melanoplus bivittatus (Say) and Melanoplus mexicanus (Sauss.) (Orthoptera: Locustidae)1

Published online by Cambridge University Press:  31 May 2012

H. L. House  and
June M. Stephens
H. L. House
Affiliation:
Entomology Laboratory, Belleville, Ontario
June M. Stephens
Affiliation:
Entomology Laboratory, Belleville, Ontario
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Extract

It was necessary to know the specific gravity of the haemolymph, or blood, of grasshoppers for certain investigations on insect immunity, This information is not given in reviews on the physical properties of insect haemolymph (Rapp, 1947; Buck, 1953). Determinations of specific gravity were made by the technique developed by Lowry and Hunter (1945) and used on insects by Baldwin and House (1352). Nine males and 11 females of Melanoplus bivittatus (Say) and 11 males and nine females of Melanoplus mexicanus (Sauss.) were used after they had fed in the laboratory for three days on lettuce and dry dietary components. The mean specific gravity and the standard deviation for M. bivittatus was 1.0271 ± .0043, 1.0262 ± .0046, for the male and 1.0281 ± .0039 for the female; for M. mexicanus, 1.0261 ± .0023, 1.0257 ±.0021, and 1.0264 ± .0025, respectively.

Type
Articles
Information
The Canadian Entomologist , Volume 90 , Issue 8 , August 1958 , pp. 463
Copyright
Copyright © Entomological Society of Canada 1958

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References

Baldwin, W. F., and House, H. L.. 1952. Factors influencing the specific gravity of insect haemolymph. Can. Ent. 84: 131–139.CrossRefGoogle Scholar
Buck, J. B. 1953. Physical properties and chemical composition of insect blood. In Insect physiology, ed. by Roeder, K. D., pp. 147–190. John Wiley and Sons, Inc. New York.Google Scholar
Lowry, C. H., and Hunter, T. H.. 1945. The determination of serum protein concentration with a gradient tube. J. Biol. Chem. 159: 465–474.CrossRefGoogle Scholar
Rapp, J. L. C. 1947. Insect haemolymph: a review. J. New York Ent. Soc. 55: 295–308.Google Scholar
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