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Pathogenicity of two new isolates of Metarhizium anisopliae from Canadian soil to Melanoplus bivittatus (Orthoptera: Acrididae) and Tenebrio molitor (Coleoptera: Tenebrionidae)

Published online by Cambridge University Press:  02 April 2012

Adil Adatia ,
Dan Johnson  and
Susan Entz
Adil Adatia
Affiliation:
Department of Geography, University of Lethbridge, 4401 University Drive, Lethbridge, Alberta, Canada T1K 3M4
Dan Johnson*
Affiliation:
Department of Geography, University of Lethbridge, 4401 University Drive, Lethbridge, Alberta, Canada T1K 3M4
Susan Entz
Affiliation:
Department of Geography, University of Lethbridge, 4401 University Drive, Lethbridge, Alberta, Canada T1K 3M4
*
1 Corresponding author (e-mail: dan.johnson@uleth.ca).
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Abstract

Worldwide biological-control research has shown that the fungal entomopathogen Metarhizium anisopliae (Metschnikoff) is an alternative to chemical insecticides for controlling grasshoppers and locusts. The pathogenicity of two recently discovered isolates of M. anisopliae var. anisopliae Driver and Milner from Canadian soil to the key grasshopper pest Melanoplus bivittatus (Say) and the yellow mealworm, Tenebrio molitor L., was determined by means of laboratory bioassays. Insects were fed a single dose of 105 conidia suspended in sunflower oil on food (a standard-size lettuce wafer). Subsequent feeding activity, movement, and mortality were assessed daily. The isolates were equally pathogenic, and similar in pathogenicity to the industry standard, Green Guard (M. anisopliae var. acridum Driver and Milner). Treatment with the three isolates resulted in 50% grasshopper mortality in 5–6 days and 90% mortality in 6–7 days.

Résumé

La recherche en lutte biologique à l'échelle globale a démontré que le champignon entomopathogène Metarhizium anisopliae (Metschnikoff) représente une solution de rechange aux insecticides chimiques pour le contrôle des criquets et des locustes. La pathogénicité de deux isolats de M. anisopliae var. anisopliae Driver et Milner découverts récemment dans du sol canadien a été déterminée dans des essais de laboratoire sur l'important criquet ravageur Melanoplus bivittatus (Say) et l'adulte du ver de farine jaune Tenebrio molitor L. Les insectes ont reçu une seule dose de 105 conidies suspendues dans de l'huile de tournesol sur de la nourriture (une pastille de laitue de taille standard). Les jours suivants, leur activité alimentaire, leurs déplacements et leur mortalité ont été notés. Les deux isolats sont également pathogènes et leur pathogénicité est semblable à celle de la norme industrielle de Green Guard (M. anisopliae var. acridum Driver and Milner). Un traitement avec les trois isolats cause une mortalité des criquets de 50 % en 5–6 jours et de 90 % en 6–7 jours.

[Traduit par la Rédaction]

Type
Articles
Information
The Canadian Entomologist , Volume 142 , Issue 2 , April 2010 , pp. 128 - 134
Copyright
Copyright © Entomological Society of Canada 2010

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